CAJ | 학술논문

为探讨凝集素提取液对刺参Apostichopus japonicus免疫的影响,将刺参基础饲料中分别添加质量分数为0·64%、1·28%、2·56%的海带凝集素提取液,或质量分数为1·88%、3·76%、7·52%的条斑紫菜凝集素提取液,分别投喂6个试验组刺参(体质量为3·50 g ±1·95 g),以基础饲料饲养组为对照,分别于投喂凝集素提取液后第4、8、12、17天时,检测各组刺参体壁中过氧化氢酶( CAT)、溶菌酶( LSZ)、碱性磷酸酶(AKP)和酸性磷酸酶(ACP)的活性。结果表明：投喂海带凝集素提取液后,各浓度组刺参CAT、 LSZ活性在第8、12、17天时均有显著或极显著提高(P<0·05或P<0·01),0·64%组ACP活性在第8、17天时有显著提高(P<0·05)；投喂条斑紫菜凝集素提取液后,各浓度组刺参CAT活性在第8、12、17天时有显著或极显著提高( P<0·05或P<0·01),7·52%组LSZ活性在第8天时有显著提高( P<0·05), AKP活性在第4、17天时有极显著提高( P<0·01), ACP活性在第12天时7·52%组有极显著提高( P<0·01),第17天时各浓度组均有显著或极显著提高(P<0·05或P<0·01)。研究表明,海带和条斑紫菜凝集素提取液均能提高剌参体壁中CAT、 LSZ、 AKP、 ACP的活性,显著增强剌参非特异性免疫水平,将其作为刺参免疫增强剂具有广阔的应用前景。
위탐토응집소제취액대자삼Apostichopus japonicus면역적영향,장자삼기출사료중분별첨가질량분수위0·64%、1·28%、2·56%적해대응집소제취액,혹질량분수위1·88%、3·76%、7·52%적조반자채응집소제취액,분별투위6개시험조자삼(체질량위3·50 g ±1·95 g),이기출사료사양조위대조,분별우투위응집소제취액후제4、8、12、17천시,검측각조자삼체벽중과양화경매( CAT)、용균매( LSZ)、감성린산매(AKP)화산성린산매(ACP)적활성。결과표명：투위해대응집소제취액후,각농도조자삼CAT、 LSZ활성재제8、12、17천시균유현저혹겁현저제고(P<0·05혹P<0·01),0·64%조ACP활성재제8、17천시유현저제고(P<0·05)；투위조반자채응집소제취액후,각농도조자삼CAT활성재제8、12、17천시유현저혹겁현저제고( P<0·05혹P<0·01),7·52%조LSZ활성재제8천시유현저제고( P<0·05), AKP활성재제4、17천시유겁현저제고( P<0·01), ACP활성재제12천시7·52%조유겁현저제고( P<0·01),제17천시각농도조균유현저혹겁현저제고(P<0·05혹P<0·01)。연구표명,해대화조반자채응집소제취액균능제고랄삼체벽중CAT、 LSZ、 AKP、 ACP적활성,현저증강랄삼비특이성면역수평,장기작위자삼면역증강제구유엄활적응용전경。
The effects of two lectin solutions extracted from kelp Laminaria japonica and laver Porphyra yezoensis Veda on activities of immunoenzymes were studied in body walls of juvenile sea cucumber Apostichopus japonicus with body weight of (3. 50±1. 95)g. The juveniles were fed basic diet (as control group) supplemented with kelp lectin extracted solution at a rate 0. 64%,1. 28% and 2. 56% (mass percent) and fed the basic diet supplemented with laver extracted solution at a rate 1. 88%, 3. 76% and 7. 52%. The activities of catalase (CAT), lysozyme (LSZ), alkaline phosphatase (AKP) and acid phosphatase (ACP) were determined in the body walls on the 4th, 8th, 12th and 17th day after the sea cucumber juveniles were fed the diets supplemented with the kelp extracted so-lution, and on the 4th, 8th, 13th and 17th day after the juveniles were fed the diets supplemented with laver ex-tracted solution. The results showed that the activities of CAT and LSZ were found to be significantly or very signifi-cantly increased in 8 , 12 , and 17 d after the juveniles were fed the diets containing kelp extracted lectin solution group(P<0. 05 or P<0. 01). The AKP activity was shown to be significantly increased in the juveniles in 12 d in 1 . 28% kelp extracted lectin solution group ( P<0 . 01 ) , and ACP activity in 8 , and 17 d in 0 . 64% kelp extracted lectin solution group ( P<0 . 05 ) . The CAT activity was significantly or very significantly increased 8 , 13 , and 17 d after the juveniles were fed supplemented with laver extracted lectin solution group(P<0. 05 or P<0. 01), LSZ ac-tivity significantly enhanced 8 d after the juveniles were fed supplemented with laver extracted lectin solution ( P<0 . 05 ) , and AKP activity very significantly increased 4 , and 17 d in 7 . 52% laver extracted lectin solution group ( P<0 . 01 ) . The activity of ACP was very significantly increased on the 12 th d in 7 . 52% laver extracted lectin so-lution group(P<0. 01), very significantly improved on the 17th d in 1. 88% and 7. 52% laver extracted lectin so-lution groups(P<0. 01), and significantly increased on the 17th d in 3. 76% laver extracted lectin solution group ( P<0 . 05 ) . The findings indicate that laver and kelp lectin extracted solution can lead to improve the activities of CAT, LSZ, AKP and ACP in body walls of sea cucumber, and thus increase in immunity, and will become a broad prospect on enhancing the immunity of sea cucumber.