CAJ | 학술논문

进行了不同温度(10、14、17℃)和不同 Cu (Ⅱ)质量浓度(7·40、32·63、56·58、193·94、300·48μg/L)的耦合作用对厚壳贻贝Mytilus coruscus D形幼虫生长和存活的急性毒性影响试验。结果表明： Cu(Ⅱ)胁迫96 h后,10、14℃试验组幼虫的存活率在Cu(Ⅱ)质量浓度为193·94、300·48μg/L时均显著降低(P<0·05),17℃试验组幼虫的存活率在Cu(Ⅱ)质量浓度为56·58μg/L及以上时显著降低(P<0·05)；在温度为10、14、17℃时 Cu(Ⅱ)胁迫的96 h 半致死浓度(96 h LC50)分别为143·6、143·5、133·0μg/L,表明随着温度的升高LC50呈下降趋势； Cu(Ⅱ)胁迫96 h的生长试验结果显示,在10℃时,56·58μg/L质量浓度组的壳长相对增长率与对照组存在显著性差异( P<0·05),14、17℃时,32·63、56·58μg/L质量浓度组的壳长相对增长率与对照组存在显著性差异( P<0·05)；在相同Cu (Ⅱ)浓度和不同水温胁迫下,17℃试验组幼虫壳长相对增长率显著高于10、14℃试验组(P<0·05)。研究表明, Cu(Ⅱ)胁迫和温度的变化能影响厚壳贻贝D形幼虫的存活,且在较高的温度下幼虫对Cu(Ⅱ)胁迫更为敏感。
진행료불동온도(10、14、17℃)화불동 Cu (Ⅱ)질량농도(7·40、32·63、56·58、193·94、300·48μg/L)적우합작용대후각이패Mytilus coruscus D형유충생장화존활적급성독성영향시험。결과표명： Cu(Ⅱ)협박96 h후,10、14℃시험조유충적존활솔재Cu(Ⅱ)질량농도위193·94、300·48μg/L시균현저강저(P<0·05),17℃시험조유충적존활솔재Cu(Ⅱ)질량농도위56·58μg/L급이상시현저강저(P<0·05)；재온도위10、14、17℃시 Cu(Ⅱ)협박적96 h 반치사농도(96 h LC50)분별위143·6、143·5、133·0μg/L,표명수착온도적승고LC50정하강추세； Cu(Ⅱ)협박96 h적생장시험결과현시,재10℃시,56·58μg/L질량농도조적각장상대증장솔여대조조존재현저성차이( P<0·05),14、17℃시,32·63、56·58μg/L질량농도조적각장상대증장솔여대조조존재현저성차이( P<0·05)；재상동Cu (Ⅱ)농도화불동수온협박하,17℃시험조유충각장상대증장솔현저고우10、14℃시험조(P<0·05)。연구표명, Cu(Ⅱ)협박화온도적변화능영향후각이패D형유충적존활,차재교고적온도하유충대Cu(Ⅱ)협박경위민감。
In this study, the combined effects of temperature ( from 10 ℃ to 17 ℃) and copper (II) concentra-tions ranging from 0 (control) to 300. 48 μg/L on survival and growth of straight-hinge veliger larvae were investi-gated in mussel Mytilus curuscus. The results showed that the larval survival in 10 and 14℃ groups was found to be significantly reduced at copper concentrations of 193 . 94 and 300 . 48 μg/L in 96 h ( P<0 . 05 ) , while the larval survival in 17 ℃ group was shown to be significantly reduced at copper concentrations ≥ 56. 58 μg/L in 96 h, with LC50 of 143. 6μg/L at 10℃, 143. 5 at 14℃ and 133. 0μg/L 17℃, indicating that the LC50 values was de-creased with warming temperature. The 96 h growth trial revealed that significant difference in the relative growth rates in shell length was only observed between the copper treated group (56. 58μg/L) and the control group at 10℃(P<0. 05). At 14 ℃ and 17 ℃, however, significant difference in the relative growth rates in shell length was found between the the copper treated groups (32. 63 and 56. 58 μg/L) and the control in 96 h(P<0. 05). Within the same copper concentration and various temperature, there was significant higher shell growth rate at 17 ℃ than at 10 ℃ and 14℃ in 96 h. The findings indicate that copper stress and changes in water temperature affect surviv-al of the straight-hinge veliger larvae, and that larvae are more sensitive to copper stress in higher temperature.