CAJ | 학술논문

以黄海和渤海春季刺参养殖池塘的沉积物、海水和刺参Apostichopus japonicus肠道内容物中的细菌基因组DNA为模板，以细菌16S rDNA通用引物进行PCR扩增，构建16S rDNA文库并进行测序分析。结果表明：黄海和渤海养殖刺参肠道内容物、池塘沉积物和海水中共存在变形菌Proteobacteria、蓝细菌门Cy：anobacteria、放线菌门Actinobacteria、拟杆菌门Bacteroidetes和厚壁菌门Firmicutes 5个门类的细菌类群，其优势类群均为变形菌(变形菌比例>41%)，且在文库所含变形菌的4个亚门( Alphaproteobacteria、 Betap：roteobacteria、 Gammaproteobacteria、 Deltaproteobacteria)中，除黄海春季刺参养殖池塘海水文库(YSp3)中α-变形菌占优势外，其余5个文库均以γ-变形菌为优势亚门。
이황해화발해춘계자삼양식지당적침적물、해수화자삼Apostichopus japonicus장도내용물중적세균기인조DNA위모판，이세균16S rDNA통용인물진행PCR확증，구건16S rDNA문고병진행측서분석。결과표명：황해화발해양식자삼장도내용물、지당침적물화해수중공존재변형균Proteobacteria、람세균문Cy：anobacteria、방선균문Actinobacteria、의간균문Bacteroidetes화후벽균문Firmicutes 5개문류적세균류군，기우세류군균위변형균(변형균비례>41%)，차재문고소함변형균적4개아문( Alphaproteobacteria、 Betap：roteobacteria、 Gammaproteobacteria、 Deltaproteobacteria)중，제황해춘계자삼양식지당해수문고(YSp3)중α-변형균점우세외，기여5개문고균이γ-변형균위우세아문。
The 16 S rDNA genes of bacteria were amplified and 16 S rDNA clone libraries were constructed based on the total DNA extracted from the intestine of and water and sediments in culture ponds of sea cucumber Apostichopus japonicus from Yellow Sea and Bohai in spring. The results showed that the bacteria in the intestine and culture pond were composed of Proteobacteria, Cyanobacteria, Actinobacteria, Bacteroidetes and Firmicutes, with domi-nant Proteobacteria (>41%) in the intestine and culture ponds. Among the four subphyla of Proteobacteria( Alpha-proteobacteria, Betaproteobacteria, Gammaproteobacteria and Deltaproteobacteria) , Alphaproteobacteria was domi-nant in the library of seawater in sea cucmber culture ponds from Yellow Sea in spring, while Gammaproteobacteria was dominant group in others.