CAJ | 학술논문

为探究急性温度胁迫对虹鳟Oncorhynchus mykiss肝脏代谢酶和部分生长调控基因表达的影响，采用血清生化分析仪测定了低温(6℃)和高温(24℃)胁迫下虹鳟(体质量为240 g依18 g)血清中谷草转氨酶( AST)、谷丙转氨酶( ALT)和碱性磷酸酶( ALP)的活性，通过放射免疫法测定了血清类胰岛素生长因子1(IGF-1)的含量，采用实时定量法测定了肝脏中GHR1、 IGF-1和IGF-2的表达量。结果表明：温度胁迫2 h后，低温和高温两个处理组鱼的AST活性和高温组的ALT活性均呈先升高后降低的趋势，均在恢复6 h时达到最大值，而低温组鱼的ALT活性在胁迫后恢复0 h时就显著升高( P<0·05)，随后逐渐降低至对照组水平；低温组鱼的ALP活性在胁迫后显著降低(P<0·05)，并在48 h的恢复期间始终维持在较低水平，而高温组鱼的ALP活性比对照组略有降低(P>0·05)；低温组鱼的血清IGF-1激素含量在恢复12 h后呈上升趋势，并在48 h时恢复至对照组水平，而高温组血清IGF-1含量在胁迫后始终处于较低水平；两个处理组肝脏中， GHR1基因表达量比对照组略有降低( P>0·05)，而IGF-1和IGF-2基因表达量均呈先降低后升高的趋势，且IGF-2表达量下降更明显，并在恢复12 h时达到最低值。研究表明：温度胁迫后，虹鳟血清转氨酶活性降低，肝脏受到损伤；血清IGF-1激素含量下降，肝脏中GHR1、 IGF-1和IGF-2基因表达量均低于对照组；高温组各指标变化较大，说明属于冷水鱼类的虹鳟受高温影响更为显著。
위탐구급성온도협박대홍준Oncorhynchus mykiss간장대사매화부분생장조공기인표체적영향，채용혈청생화분석의측정료저온(6℃)화고온(24℃)협박하홍준(체질량위240 g의18 g)혈청중곡초전안매( AST)、곡병전안매( ALT)화감성린산매( ALP)적활성，통과방사면역법측정료혈청류이도소생장인자1(IGF-1)적함량，채용실시정량법측정료간장중GHR1、 IGF-1화IGF-2적표체량。결과표명：온도협박2 h후，저온화고온량개처리조어적AST활성화고온조적ALT활성균정선승고후강저적추세，균재회복6 h시체도최대치，이저온조어적ALT활성재협박후회복0 h시취현저승고( P<0·05)，수후축점강저지대조조수평；저온조어적ALP활성재협박후현저강저(P<0·05)，병재48 h적회복기간시종유지재교저수평，이고온조어적ALP활성비대조조략유강저(P>0·05)；저온조어적혈청IGF-1격소함량재회복12 h후정상승추세，병재48 h시회복지대조조수평，이고온조혈청IGF-1함량재협박후시종처우교저수평；량개처리조간장중， GHR1기인표체량비대조조략유강저( P>0·05)，이IGF-1화IGF-2기인표체량균정선강저후승고적추세，차IGF-2표체량하강경명현，병재회복12 h시체도최저치。연구표명：온도협박후，홍준혈청전안매활성강저，간장수도손상；혈청IGF-1격소함량하강，간장중GHR1、 IGF-1화IGF-2기인표체량균저우대조조；고온조각지표변화교대，설명속우랭수어류적홍준수고온영향경위현저。
Activities of serum aspartate aminotransferase ( AST ) , alanine aminotransferase ( ALT ) and alkaline phosphatase (ALP) were determined in rainbow trout Oncorhynchus mykiss with body weight of (240 g±18 g)ex-posed to low (6℃) and high temperature(24℃) by a BS-180 biochemical analyzer, and levels of serum insulin-like growth factor-1 ( IGF-1 ) and the expression of GHR1 , IGF-1 and IGF-2 in liver were detected by radioim-munoassay and by real-time quantification. The activities of serum AST and ALT were shown to be increased first and then decreased, the maximum at 6 h and significantly higher in the lower temperature group for ALT even at 0 h(P<0. 05), finally being reduced to the level in the control group. The ALP activity was significantly decreased at 0 h (P<0. 05), and then maintained low level in low temperature group, while the ALP activity was decreased slightly in high temperature group. The serum IGF-1 level was found increase at 0 h and then recovery to the level in the control group at low temperature exposure. In the high temperature exposure, however, the serum IGF-1 level was kept at low level, without significant difference (P>0. 05). The expression of IGF-1 and IGF-2 genes was shown to be decreased firstly and then increased, the minimum at 12 h, significantly lower for IGF-2 gene. The findings revealed that acute temperature led to harmful liver and to increase in AST and ALT activities and to decrease in ALP activity;meanwhile serum IGF-1 level and the expression of GHR1 , IGF-1 and IGF-2 also were decreased, indicating that acute temperature may affect the expression of growth related genes.