山东体育学院学报
山東體育學院學報
산동체육학원학보
JOURNAL OF SHANDONG PHYSICAL EDUCATION INSTITUTE
2014年
6期
87-91
,共5页
大蒜素%DNA损伤%氧化应激%力竭运动
大蒜素%DNA損傷%氧化應激%力竭運動
대산소%DNA손상%양화응격%력갈운동
allicin%DNA damage%oxidative stress%exhaustive exercise
目的：观察补充大蒜素对人体力竭运动后外周血细胞DNA的损伤效应及血清中SOD、GSH和MDA活性的影响，探讨大蒜素抗DNA氧化损伤的作用机制。方法：选取16名健康男运动员（年龄21.06±1.02岁），随机分为两组包括对照组8名（C组，补充淀粉安慰剂胶囊）和实验组8名（ T组，补充大蒜素胶囊），运动前14天各组每天分别口服安慰剂和大蒜素胶囊，补剂后所有被试均参加Bruce力竭运动方案，并分别在补剂前、补剂后和运动后即刻这三个阶段对两组受试者进行采血抗凝，采用单细胞凝胶电泳（ SCGE）技术检测人体外周血细胞DNA损伤情况，并测定血浆中超氧化物岐化酶（ SOD）活性、谷胱甘肽（ GSH）和丙二醛（ MDA）含量。结果：补剂前和补剂后，对照组和实验组的DNA损伤水平与氧化应激水平无显著性差异（ P>0.05）；运动后即刻实验组运动员外周血细胞的DNA损伤水平显著低于对照组力竭运动后的水平，其MDA水平、SOD和GSH含量较力竭对照组亦出现显著降低（ P<0.001，P<0.05，P<0.05）；而对照组运动后即刻SOD、GSH和 MDA水平较运动前均出现显著上升，DNA损伤值明显加剧（ P<0.001）。结论：力竭运动可诱导人体外周血细胞DNA损伤值增加及脂质过氧化产物MDA水平的升高，介导SOD和GSH抗氧化酶活性的过量表达，大蒜素能够提高机体抗氧化能力，降低自由基水平，减轻和预防运动性氧应激所致的DNA损伤。
目的：觀察補充大蒜素對人體力竭運動後外週血細胞DNA的損傷效應及血清中SOD、GSH和MDA活性的影響，探討大蒜素抗DNA氧化損傷的作用機製。方法：選取16名健康男運動員（年齡21.06±1.02歲），隨機分為兩組包括對照組8名（C組，補充澱粉安慰劑膠囊）和實驗組8名（ T組，補充大蒜素膠囊），運動前14天各組每天分彆口服安慰劑和大蒜素膠囊，補劑後所有被試均參加Bruce力竭運動方案，併分彆在補劑前、補劑後和運動後即刻這三箇階段對兩組受試者進行採血抗凝，採用單細胞凝膠電泳（ SCGE）技術檢測人體外週血細胞DNA損傷情況，併測定血漿中超氧化物岐化酶（ SOD）活性、穀胱甘肽（ GSH）和丙二醛（ MDA）含量。結果：補劑前和補劑後，對照組和實驗組的DNA損傷水平與氧化應激水平無顯著性差異（ P>0.05）；運動後即刻實驗組運動員外週血細胞的DNA損傷水平顯著低于對照組力竭運動後的水平，其MDA水平、SOD和GSH含量較力竭對照組亦齣現顯著降低（ P<0.001，P<0.05，P<0.05）；而對照組運動後即刻SOD、GSH和 MDA水平較運動前均齣現顯著上升，DNA損傷值明顯加劇（ P<0.001）。結論：力竭運動可誘導人體外週血細胞DNA損傷值增加及脂質過氧化產物MDA水平的升高，介導SOD和GSH抗氧化酶活性的過量錶達，大蒜素能夠提高機體抗氧化能力，降低自由基水平，減輕和預防運動性氧應激所緻的DNA損傷。
목적：관찰보충대산소대인체력갈운동후외주혈세포DNA적손상효응급혈청중SOD、GSH화MDA활성적영향，탐토대산소항DNA양화손상적작용궤제。방법：선취16명건강남운동원（년령21.06±1.02세），수궤분위량조포괄대조조8명（C조，보충정분안위제효낭）화실험조8명（ T조，보충대산소효낭），운동전14천각조매천분별구복안위제화대산소효낭，보제후소유피시균삼가Bruce력갈운동방안，병분별재보제전、보제후화운동후즉각저삼개계단대량조수시자진행채혈항응，채용단세포응효전영（ SCGE）기술검측인체외주혈세포DNA손상정황，병측정혈장중초양화물기화매（ SOD）활성、곡광감태（ GSH）화병이철（ MDA）함량。결과：보제전화보제후，대조조화실험조적DNA손상수평여양화응격수평무현저성차이（ P>0.05）；운동후즉각실험조운동원외주혈세포적DNA손상수평현저저우대조조력갈운동후적수평，기MDA수평、SOD화GSH함량교력갈대조조역출현현저강저（ P<0.001，P<0.05，P<0.05）；이대조조운동후즉각SOD、GSH화 MDA수평교운동전균출현현저상승，DNA손상치명현가극（ P<0.001）。결론：력갈운동가유도인체외주혈세포DNA손상치증가급지질과양화산물MDA수평적승고，개도SOD화GSH항양화매활성적과량표체，대산소능구제고궤체항양화능력，강저자유기수평，감경화예방운동성양응격소치적DNA손상。
Objective:This study was performed to determine the effect of allicin against DNA damage and oxidative stress on human peripheral blood cell after exhaustive exercise,and discuss the protec-tive mechanism of allicin. Methods:16 male athletes( aged 21. 06 ± 1. 02 years),in a randomized and double-blind design were allocated in two equal supplement and placebo groups( 80 mg/day allicin for 14 days). After supplementation,all participants were participated in Bruce test. The blood samples were taken in three phases( before and after the supplementation and after the exer-cise). The DNA damage was investigated on human peripheral blood cell by using the single cell gel electrophoresis( SCGE)and the level of SOD,GSH and MDA was detected in serum. Results:Dur-ing the first two phases( before and after the supplementation ),the level of DNA damage and the status of SOD,GSH and MDA between supplement and placebo groups did not show significant difference( P >0. 05 ). Moreover,exercise -induced decrease of DNA damage and the level of SOD,GSH and MDA in the supplement group were significantly more in comparison with those in the placebo group(P<0. 001),instead,the DNA damage and the level of SOD,GSH and MDA in-creased significantly after exercise. Conclusions:The exhaustive exercise could result in the DNA damage on human peripheral blood cell and induce high level of oxidative stress. However,the alli-cin could reduce free radicals and prevent DNA damage from oxidative stress induced by exhaustive exercise.
