CAJ | 학술논문

目的了解多重耐药鲍曼不动杆菌整合子的表达及耐药基因的携带情况。方法收集2012年8—10月某院临床分离的51株多重耐药鲍曼不动杆菌，对其进行药敏试验；采用聚合酶链反应（PCR）检测Ⅰ、Ⅱ、Ⅲ类整合酶基因及整合子可变区基因盒，应用限制性片段长度多态性（RFLP）分析和测序结果进行同源性分析。结果多重耐药鲍曼不动杆菌整合酶基因阳性率为78．43％（40／51），均为Ⅰ类整合子，未检出Ⅱ、Ⅲ类整合子。Ⅰ类整合子阳性菌中，39株（97．50％）扩增出可变区，检出5种耐药基因盒组合形式，分别为 aacA4（14株）、aacA4＋catB8（22株）、arr－3＋aacA4（1株）、dfrA15（1株）和 arr－3（1株）。结论该院临床分离的多重耐药鲍曼不动杆菌的耐药性可能与Ⅰ类整合子表达有关。Ⅰ类整合子主要携带 aacA4、aacA4＋catB8、arr－3＋aacA4、dfrA15和 arr－3基因盒。
목적료해다중내약포만불동간균정합자적표체급내약기인적휴대정황。방법수집2012년8—10월모원림상분리적51주다중내약포만불동간균，대기진행약민시험；채용취합매련반응（PCR）검측Ⅰ、Ⅱ、Ⅲ류정합매기인급정합자가변구기인합，응용한제성편단장도다태성（RFLP）분석화측서결과진행동원성분석。결과다중내약포만불동간균정합매기인양성솔위78．43％（40／51），균위Ⅰ류정합자，미검출Ⅱ、Ⅲ류정합자。Ⅰ류정합자양성균중，39주（97．50％）확증출가변구，검출5충내약기인합조합형식，분별위 aacA4（14주）、aacA4＋catB8（22주）、arr－3＋aacA4（1주）、dfrA15（1주）화 arr－3（1주）。결론해원림상분리적다중내약포만불동간균적내약성가능여Ⅰ류정합자표체유관。Ⅰ류정합자주요휴대 aacA4、aacA4＋catB8、arr－3＋aacA4、dfrA15화 arr－3기인합。
Objective To investigate the expression and resistant gene of integron in multidrug-resistant Acinetobacter baumannii (MDR-Ab).Methods 51 strains of MDR-Ab isolated from a hospital in August-October 2012 were collected, antimicrobial susceptibility testing was performed.Class I(Int I),II (Int II)and III (Int III)of integrase genes and inte-gron variable region gene cassettes were detected by polymerase chain reaction (PCR),and the homology of integron varia-ble region was analyzed by detection results of restriction fragment length polymorphism (RFLP)and DNA sequencing. Results Positive rate of integrase gene in MDR-Ab was 78.43%(40/51).All genes belonged to Int I,while IntⅡand IntⅢ were not found.Variable region cassettes were detected in 97.50% (n=39)of Int I,there were 5 types of integron gene cassettes:aacA4 in 14 strains,aacA4+catB8 in 22 strains,arr-3 +aacA4 in 1 strain,dfrA15 in 1 strain and arr-3 in 1 strain.Conclusion MDR-Ab isolated from this hospital may be related with Int I expression.Int I carried gene cassettes as follows:aacA4,aacA4+catB8,arr-3+aacA4,dfrA15 and arr-3.