河南科学
河南科學
하남과학
HENAN SCIENCE
2014年
12期
2477-2480
,共4页
6-巯基嘌呤%金纳米粒子%分光光度法
6-巰基嘌呤%金納米粒子%分光光度法
6-구기표령%금납미입자%분광광도법
6-mercaptopurine%gold nanoparticles%spectrophotometry
在pH 5.5的B-R缓冲溶液中,6-巯基嘌呤与纳米金反应后,纳米金粒子溶液的颜色由酒红色逐渐变为蓝色,其最大吸收波长从525 nm移至670 nm .670 nm和525 nm处吸光度的比值(A670/A525)与6-巯基嘌呤的浓度呈正比.据此,在优化实验条件下,建立纳米金分光光度法测量痕量6-巯基嘌呤的新方法.该方法准确度高、灵敏度好.
在pH 5.5的B-R緩遲溶液中,6-巰基嘌呤與納米金反應後,納米金粒子溶液的顏色由酒紅色逐漸變為藍色,其最大吸收波長從525 nm移至670 nm .670 nm和525 nm處吸光度的比值(A670/A525)與6-巰基嘌呤的濃度呈正比.據此,在優化實驗條件下,建立納米金分光光度法測量痕量6-巰基嘌呤的新方法.該方法準確度高、靈敏度好.
재pH 5.5적B-R완충용액중,6-구기표령여납미금반응후,납미금입자용액적안색유주홍색축점변위람색,기최대흡수파장종525 nm이지670 nm .670 nm화525 nm처흡광도적비치(A670/A525)여6-구기표령적농도정정비.거차,재우화실험조건하,건립납미금분광광도법측량흔량6-구기표령적신방법.해방법준학도고、령민도호.
In the B-R pH 5.5 buffer solution,6-mercaptopurine(6-MP)and gold nanoparticles after reaction,gold nanoparticle solution color changed from red to blue,the maximum absorption wavelength redshift from 525 nm to 670 nm. The ratios of 670 nm and 525 nm absorbance(A670/A525)was proportional to the concentration of 6-mercaptopurine. Accordingly,under the optimized experimental conditions,a new method of trace 6-mercaptopurine was established with nano-gold photometric method. The method was simple,accurate and sensitive.