中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
1期
166-168
,共3页
王小林%余敏灵%陈文秋%封家福
王小林%餘敏靈%陳文鞦%封傢福
왕소림%여민령%진문추%봉가복
离子色谱法%山药%氢氧化钾溶液%直接提取%二氧化硫%含量测定
離子色譜法%山藥%氫氧化鉀溶液%直接提取%二氧化硫%含量測定
리자색보법%산약%경양화갑용액%직접제취%이양화류%함량측정
Ion chromatography%Rhizoma dioscoreae%Potassium hydroxide solution%Direct extraction%Sulfite anhydride%Con-tent determination
目的：：建立离子色谱法测定山药中二氧化硫含量的方法。方法：采用氢氧化钾溶液(25 mmol·L-1)直接提取,色谱柱为IonPac? AS11-HC色谱柱(250 mm ×4 mm,9.0μm)；柱温20℃；淋洗液：氢氧化钾溶液(20 mmol·L-1)；淋洗液流速1.0 ml·min-1；电导池温度20℃。结果：亚硫酸根标准溶液进样量在1.160~29.100μg范围内与亚硫酸根色谱峰的峰面积有良好的线性关系(r=0.9999),平均回收率为98.9%(RSD=0.6%, n=9),最低定量限为1.38 ng·ml-1。结论：本方法简便、准确、快速,适用于山药中二氧化硫的定量检测。
目的：：建立離子色譜法測定山藥中二氧化硫含量的方法。方法：採用氫氧化鉀溶液(25 mmol·L-1)直接提取,色譜柱為IonPac? AS11-HC色譜柱(250 mm ×4 mm,9.0μm)；柱溫20℃；淋洗液：氫氧化鉀溶液(20 mmol·L-1)；淋洗液流速1.0 ml·min-1；電導池溫度20℃。結果：亞硫痠根標準溶液進樣量在1.160~29.100μg範圍內與亞硫痠根色譜峰的峰麵積有良好的線性關繫(r=0.9999),平均迴收率為98.9%(RSD=0.6%, n=9),最低定量限為1.38 ng·ml-1。結論：本方法簡便、準確、快速,適用于山藥中二氧化硫的定量檢測。
목적：：건립리자색보법측정산약중이양화류함량적방법。방법：채용경양화갑용액(25 mmol·L-1)직접제취,색보주위IonPac? AS11-HC색보주(250 mm ×4 mm,9.0μm)；주온20℃；림세액：경양화갑용액(20 mmol·L-1)；림세액류속1.0 ml·min-1；전도지온도20℃。결과：아류산근표준용액진양량재1.160~29.100μg범위내여아류산근색보봉적봉면적유량호적선성관계(r=0.9999),평균회수솔위98.9%(RSD=0.6%, n=9),최저정량한위1.38 ng·ml-1。결론：본방법간편、준학、쾌속,괄용우산약중이양화류적정량검측。
Objective:To establish a quantitative analysis method to determine the content of sulfurous anhydride in Rhizoma di-oscoreae by ion chromatography-direct extraction. Methods:Sulfurous anhydride was extracted by KOH solution (25 mmol·L-1). An IonPac? AS11-HC column(250 mm × 4 mm, 9. 0 μm) was used. The column temperature was 20℃, the eluent was KOH solution (20 mmol·L-1 ) at flow rate of 1. 00 ml·min-1 and the conductivity temperature was 20℃. Results:There was a good linear rela-tionship between the injection quantity (1.160-29.100 μg)and the peak area of sulfite(r =0.999 9). The average recovery was 98. 9%(RSD=0. 6%, n=9). The quantitation limit was 1. 38 ng·ml-1. Conclusion: The method is simple, accurate and rapid, which is appropriate for the quantitative analysis of sulfite anhydride in Rhizoma dioscoreae.
