CAJ | 학술논문

目的：：建立超高效液相色谱法测定一捻金中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量的方法。方法：色谱柱：ZORBAX Eclipse Plus C18柱(2.1 mm ×50 mm,1.8μm)；流速：0.21 ml·min-1；柱温：30℃；流动相：乙腈-水梯度洗脱；检测波长：203 nm；进样量：1μl。结果：人参皂苷Rg1在浓度为0.0203~0.3039 mg·ml-1时线性关系良好(r=0.9996),平均回收率为99.05%,RSD为1.3%(n=6)；人参皂苷Re在浓度为0.0202~0.3027 mg·ml-1时线性关系良好(r=0.9998),平均回收率为101.31%,RSD为1.1%(n=6)；人参皂苷Rb1在浓度为0.0203~0.3051 mg·ml-1时线性关系良好,r=0.9998,平均回收率为100.71%,RSD为0.9%(n=6)。结论：该方法简单、准确,可同时测定3种人参皂苷的含量,可作为一捻金的质量控制。
목적：：건립초고효액상색보법측정일념금중인삼조감Rg1、인삼조감Re、인삼조감Rb1함량적방법。방법：색보주：ZORBAX Eclipse Plus C18주(2.1 mm ×50 mm,1.8μm)；류속：0.21 ml·min-1；주온：30℃；류동상：을정-수제도세탈；검측파장：203 nm；진양량：1μl。결과：인삼조감Rg1재농도위0.0203~0.3039 mg·ml-1시선성관계량호(r=0.9996),평균회수솔위99.05%,RSD위1.3%(n=6)；인삼조감Re재농도위0.0202~0.3027 mg·ml-1시선성관계량호(r=0.9998),평균회수솔위101.31%,RSD위1.1%(n=6)；인삼조감Rb1재농도위0.0203~0.3051 mg·ml-1시선성관계량호,r=0.9998,평균회수솔위100.71%,RSD위0.9%(n=6)。결론：해방법간단、준학,가동시측정3충인삼조감적함량,가작위일념금적질량공제。
Objective:To establish a UPLC method for the determination of ginsenoside Rg1 , ginsenoside Re and ginsenoside Rb1 in Yi’ nianjin. Methods: The column was ZORBAX Eclipse Plus C18 (2. 1 mm × 50 mm,1. 8 μm), the flow rate was 0. 21 ml· min-1 , the column temperature was 30℃, the mobile phase consisted of acetonitrile-water with gradient elution, the detection wave-length was 203nm, and the sample size was 1μl. Results:The linear range of ginsenoside Rg1 was 0. 020 3-0. 303 9 mg·ml-1 ( r=0. 999 6), and the average recovery was 99. 05% (RSD=1. 3%, n=6). The linear range of ginsenoside Re was 0. 020 2-0. 302 7 mg·ml-1(r=0. 999 8), and the average recovery was 101. 31% (RSD=1. 1%, n=6). The linear range of ginsenoside Rb1 was 0. 020 3-0. 305 1 mg·ml-1(r=0. 999 8), and the average recovery of 100. 71% (RSD=0. 9%, n=6). Conclusion: The method is simple and accurate, and can determine the three components simultaneously. The method can be used in the quality control of Yi’ nianjin.