中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
1期
156-158
,共3页
傅里叶近红外光谱%透射光谱%鼻炎喷剂%盐酸麻黄碱
傅裏葉近紅外光譜%透射光譜%鼻炎噴劑%鹽痠痳黃堿
부리협근홍외광보%투사광보%비염분제%염산마황감
Fourier transmittance near-infrared ( FT-NIR) spectroscopy%Transmission spectra%Biyan sprays%Ephedrine hydro-chloride
目的：：建立快速测定鼻炎喷剂中盐酸麻黄碱含量的分析方法。方法：采用傅里叶近红外( FT-NIR)液体透射光谱法采集样品的近红外光谱。以高效液相色谱法测得值为参考,运用偏最小二乘法,以一阶微分(1d)+Norris平滑为预处理方法,谱段选择6136.38~5364.99 cm-1和7038.90~6969.48cm-1建立定量校正模型。结果：定量模型校正集的R2为0.9926,校正集验证均方差(RMSEC)为1.20；验证集的R2为0.9935,外部验证均方差(RMSEP)为1.28,内部交叉验证R2为0.9869,内部交叉验证均方差(RMSECV)为1.60。结论：所建立的定量模型具有满意的拟合效果和预测能力,能快速检测鼻炎喷剂中盐酸麻黄碱的含量,可推广应用于生产过程中的在线分析。
目的：：建立快速測定鼻炎噴劑中鹽痠痳黃堿含量的分析方法。方法：採用傅裏葉近紅外( FT-NIR)液體透射光譜法採集樣品的近紅外光譜。以高效液相色譜法測得值為參攷,運用偏最小二乘法,以一階微分(1d)+Norris平滑為預處理方法,譜段選擇6136.38~5364.99 cm-1和7038.90~6969.48cm-1建立定量校正模型。結果：定量模型校正集的R2為0.9926,校正集驗證均方差(RMSEC)為1.20；驗證集的R2為0.9935,外部驗證均方差(RMSEP)為1.28,內部交扠驗證R2為0.9869,內部交扠驗證均方差(RMSECV)為1.60。結論：所建立的定量模型具有滿意的擬閤效果和預測能力,能快速檢測鼻炎噴劑中鹽痠痳黃堿的含量,可推廣應用于生產過程中的在線分析。
목적：：건립쾌속측정비염분제중염산마황감함량적분석방법。방법：채용부리협근홍외( FT-NIR)액체투사광보법채집양품적근홍외광보。이고효액상색보법측득치위삼고,운용편최소이승법,이일계미분(1d)+Norris평활위예처리방법,보단선택6136.38~5364.99 cm-1화7038.90~6969.48cm-1건립정량교정모형。결과：정량모형교정집적R2위0.9926,교정집험증균방차(RMSEC)위1.20；험증집적R2위0.9935,외부험증균방차(RMSEP)위1.28,내부교차험증R2위0.9869,내부교차험증균방차(RMSECV)위1.60。결론：소건립적정량모형구유만의적의합효과화예측능력,능쾌속검측비염분제중염산마황감적함량,가추엄응용우생산과정중적재선분석。
Objective:To establish a rapid analysis method for determining the content of ephedrine in Biyan sprays. Methods:The FT-NIR spectra of the samples were collected by near-infrared liquid transmission spectroscopy. Using the HPLC analysis values as the reference, a quantitative analysis model for ephedrine was established with partial least square ( PLS) , the first derivative and Nor-ris smooth was used in the spectra pretreatment, and 6 136. 38-5 364. 99 cm-1 and 7 038. 90-6 969. 48 cm-1 were selected as the fre-quency ranges. Results:R2 and RMSEC of the calibration set was 0. 992 6 and 1. 20, respectively. R2 and RMSEP of the vallidation set was 0. 993 5 and 1. 28, respectively. R2 and RMSEP of the cross validation set was 0. 986 9 and 1. 60, respectively. Conclusion:The method is rapid and non-desturctive, and can be applied in the rapid assessment and online examination of the quality of Biyan sprays.
