CAJ | 학술논문

目的：：对龙胆泻肝丸(大蜜丸)薄层色谱鉴别方法进行研究。方法：采用TLC法,样品用70%甲醇加热回流提取,依次用不同极性的试剂石油醚、乙酸乙酯、正丁醇萃取。石油醚部分化合物,365 nm波长荧光检视,可以检出当归；1%香草醛硫酸显色,可以检出泽泻。乙酸乙酯部分化合物,365 nm波长荧光检视,可以检出黄芩。正丁醇部分化合物,展开剂为三氯甲烷-甲醇-水(30∶12∶3),可以同时检出柴胡和甘草；展开剂为丙酮：乙酸乙酯：水(6∶6∶1),可以检出龙胆苦苷、栀子苷和甘草苷。结果：薄层色谱斑点清晰,分离度好,专属性强,重复性好。结论：本方法可以准确地进行定性鉴别,能够用于龙胆泻肝丸(大蜜丸)的质量控制。
목적：：대룡담사간환(대밀환)박층색보감별방법진행연구。방법：채용TLC법,양품용70%갑순가열회류제취,의차용불동겁성적시제석유미、을산을지、정정순췌취。석유미부분화합물,365 nm파장형광검시,가이검출당귀；1%향초철류산현색,가이검출택사。을산을지부분화합물,365 nm파장형광검시,가이검출황금。정정순부분화합물,전개제위삼록갑완-갑순-수(30∶12∶3),가이동시검출시호화감초；전개제위병동：을산을지：수(6∶6∶1),가이검출룡담고감、치자감화감초감。결과：박층색보반점청석,분리도호,전속성강,중복성호。결론：본방법가이준학지진행정성감별,능구용우룡담사간환(대밀환)적질량공제。
Objective:To research the TLC identification method for Longdan Xiegan pills( honey pills) . Methods:TLC was used in the identification. The samples were extracted by 70% methanol with a heating reflux method, and then extracted by the agents with dif-ferent polarity, including petroleum ether, ethyl acetate and butanol. The petroleum ether part was detected by fluorescence at 365nm for Angelica sinensis, and 1% vanillin-sulfuric acid color reaction was used to detect Alisma orientale. The ethyl acetate part was determined by fluorescence at 365nm for Scutellaria baicalensis, and the butanol part was detected with chloroform-methanol-water (30∶12∶3) as the developing solvent for Bupleurum and Glycyrrhiza, and with acetone-ethyl acetate-water (6∶6∶1) as the developing solvent for gentiopi-croside, geniposide and liquiritin. Results:The developed TLC spots were clear with good separation, high specificity and promising re-producibility. Conclusion:The method can be exactly used in the qualitative identification and quality control of Longdan Xiegan pills ( honey pills) .