中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
1期
55-57,58
,共4页
复方黄根颗粒(无糖型)%三七皂苷R1%人参皂苷Rg1%人参皂苷Rb1%高效液相色谱法
複方黃根顆粒(無糖型)%三七皂苷R1%人參皂苷Rg1%人參皂苷Rb1%高效液相色譜法
복방황근과립(무당형)%삼칠조감R1%인삼조감Rg1%인삼조감Rb1%고효액상색보법
Compound Huanggen granules ( sugar free)%Notoginsenoside R1%Ginsenoside Rg1%Ginsenoside Rb1%HPLC
目的：：建立复方黄根颗粒(无糖型)中有效成分三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量测定方法。方法：色谱柱为Agilent ZORBAX SB-C18(250 mm ×4.6 mm,5μm),以乙腈-水梯度洗脱,流速为1.0 ml·min-1,检测波长为203 nm,柱温25℃,进样量为10μl。结果：三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的线性范围分别为1.6~10.0μg·ml-1( r=0.9996)、6.3~39.3μg·ml-1(r=0.9998)和6.3~39.7μg·ml-1(r=0.9997),平均加样回收率分别为98.81%(RSD=1.20%)、99.93%(RSD=0.93%)和99.22%(RSD=0.87%)(n=6)。结论：本方法操作简便、重复性好,可以更有效地控制该制剂的质量。
目的：：建立複方黃根顆粒(無糖型)中有效成分三七皂苷R1、人參皂苷Rg1和人參皂苷Rb1的含量測定方法。方法：色譜柱為Agilent ZORBAX SB-C18(250 mm ×4.6 mm,5μm),以乙腈-水梯度洗脫,流速為1.0 ml·min-1,檢測波長為203 nm,柱溫25℃,進樣量為10μl。結果：三七皂苷R1、人參皂苷Rg1和人參皂苷Rb1的線性範圍分彆為1.6~10.0μg·ml-1( r=0.9996)、6.3~39.3μg·ml-1(r=0.9998)和6.3~39.7μg·ml-1(r=0.9997),平均加樣迴收率分彆為98.81%(RSD=1.20%)、99.93%(RSD=0.93%)和99.22%(RSD=0.87%)(n=6)。結論：本方法操作簡便、重複性好,可以更有效地控製該製劑的質量。
목적：：건립복방황근과립(무당형)중유효성분삼칠조감R1、인삼조감Rg1화인삼조감Rb1적함량측정방법。방법：색보주위Agilent ZORBAX SB-C18(250 mm ×4.6 mm,5μm),이을정-수제도세탈,류속위1.0 ml·min-1,검측파장위203 nm,주온25℃,진양량위10μl。결과：삼칠조감R1、인삼조감Rg1화인삼조감Rb1적선성범위분별위1.6~10.0μg·ml-1( r=0.9996)、6.3~39.3μg·ml-1(r=0.9998)화6.3~39.7μg·ml-1(r=0.9997),평균가양회수솔분별위98.81%(RSD=1.20%)、99.93%(RSD=0.93%)화99.22%(RSD=0.87%)(n=6)。결론：본방법조작간편、중복성호,가이경유효지공제해제제적질량。
Objective:To set up a method for simultaneously determining the content of notoginsenoside R1 , ginsenoside Rg1 and ginsenoside Rb1 in compound Huanggen granules ( sugar free) . Methods:The HPLC method was carried out on an Agilent ZORBAX SB-C18(250 mm ×4.6 mm,5 μm)column. The mobile phase was acetonitrile-water with gradient elution. The flow rate was 1.0 ml· min-1 . The detection wavelength was at 203 nm. The column temperature was set at 25℃ and the sample size was 10 μl. Results:The linearity of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 was within the range of 1. 6-10. 0 μg·ml-1(r=0. 999 6), 6. 3-39. 3 μg·ml-1(r=0. 999 8) and 6. 3-39. 7 μg·ml-1(r=0. 999 7), respectively. The average recovery was 98. 81%(RSD=1. 20%),99. 93%(RSD=0. 93%) and 99. 22%(RSD=0. 87%)(n=6) , respectively. Conclusion: The method is simple, repro-ducible and specific, which can be used in the quality control of the preparation.
