中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
1期
44-46
,共3页
赵端玮%武雪%宋平顺%赵建邦
趙耑瑋%武雪%宋平順%趙建邦
조단위%무설%송평순%조건방
红芪%高效液相色谱%毛蕊异黄酮葡萄糖苷%金雀异黄酮%芒柄花素%美迪紫檀素
紅芪%高效液相色譜%毛蕊異黃酮葡萄糖苷%金雀異黃酮%芒柄花素%美迪紫檀素
홍기%고효액상색보%모예이황동포도당감%금작이황동%망병화소%미적자단소
Radix Hedysari%HPLC%Calycosin-7-glucoside%Genistein%Formononetin%Medicarpin
目的：：建立HPLC法测定红芪中毛蕊异黄酮葡萄糖苷、金雀异黄酮、芒柄花素、美迪紫檀素4种主要黄酮类成分的方法。方法：采用HPLC法,样品经甲醇回流,采用Waters公司SunFire C18色谱柱(250 mm ×4.6 mm,5μm),乙腈-0.2%磷酸水为流动相进行梯度洗脱,流速为1.0 ml·min-1,检测波长为240 nm,柱温为35℃,进样量为20μl。结果：毛蕊异黄酮葡萄糖苷在0.035~1.042μg(r=0.9996),金雀异黄酮在0.027~0.821μg(r=0.9997),芒柄花素在0.031~0.941μg(r=0.9999),美迪紫檀素在0.025~0.745μg(r=0.9992)范围内均成良好的线性关系。毛蕊异黄酮葡萄糖苷、金雀异黄酮、芒柄花素和美迪紫檀素的加样回收率分别为100.32%(RSD=1.87%),99.3%(RSD=1.76%),100.5%(RSD=1.48%),99.2%(RSD=1.45%)(n=6)。结论：该方法分析时间短、稳定性和准确度良好,对红芪药材的质量控制具有一定的参考价值。
目的：：建立HPLC法測定紅芪中毛蕊異黃酮葡萄糖苷、金雀異黃酮、芒柄花素、美迪紫檀素4種主要黃酮類成分的方法。方法：採用HPLC法,樣品經甲醇迴流,採用Waters公司SunFire C18色譜柱(250 mm ×4.6 mm,5μm),乙腈-0.2%燐痠水為流動相進行梯度洗脫,流速為1.0 ml·min-1,檢測波長為240 nm,柱溫為35℃,進樣量為20μl。結果：毛蕊異黃酮葡萄糖苷在0.035~1.042μg(r=0.9996),金雀異黃酮在0.027~0.821μg(r=0.9997),芒柄花素在0.031~0.941μg(r=0.9999),美迪紫檀素在0.025~0.745μg(r=0.9992)範圍內均成良好的線性關繫。毛蕊異黃酮葡萄糖苷、金雀異黃酮、芒柄花素和美迪紫檀素的加樣迴收率分彆為100.32%(RSD=1.87%),99.3%(RSD=1.76%),100.5%(RSD=1.48%),99.2%(RSD=1.45%)(n=6)。結論：該方法分析時間短、穩定性和準確度良好,對紅芪藥材的質量控製具有一定的參攷價值。
목적：：건립HPLC법측정홍기중모예이황동포도당감、금작이황동、망병화소、미적자단소4충주요황동류성분적방법。방법：채용HPLC법,양품경갑순회류,채용Waters공사SunFire C18색보주(250 mm ×4.6 mm,5μm),을정-0.2%린산수위류동상진행제도세탈,류속위1.0 ml·min-1,검측파장위240 nm,주온위35℃,진양량위20μl。결과：모예이황동포도당감재0.035~1.042μg(r=0.9996),금작이황동재0.027~0.821μg(r=0.9997),망병화소재0.031~0.941μg(r=0.9999),미적자단소재0.025~0.745μg(r=0.9992)범위내균성량호적선성관계。모예이황동포도당감、금작이황동、망병화소화미적자단소적가양회수솔분별위100.32%(RSD=1.87%),99.3%(RSD=1.76%),100.5%(RSD=1.48%),99.2%(RSD=1.45%)(n=6)。결론：해방법분석시간단、은정성화준학도량호,대홍기약재적질량공제구유일정적삼고개치。
Objective:To establish an HPLC method for the determination of calycosin-7-glucoside, genistein, formononetin and medicarpin in Radix Hedysari. Methods: The sample was refluxed with methanol in a water bath. The HPLC was performed on an SunFire C18 column(250 mm × 4. 6 mm,5 μm) with acetonitrile-0. 2% H3 PO4 as the mobile phase by gradient elution. The flow rate was 1. 0 ml·min-1 and the column temperature was at 35℃. The detection wavelength was 240 nm and the sample size was 20 μl. Results:The linear range of calycosin-7-glucoside was 0.035-1.042 μg(r=0.999 6), that of genistein was 0.027-0.821 μg(r=0. 999 7), that of formononetin was 0. 031-0. 941 μg(r=0. 999 9) and that of medicarpin was 0. 025-0. 745 μg(r=0. 999 6). The average recovery of calycosin-7-glucoside, genistein, formononetin and medicarpin was 100. 32%(RSD=1. 87%), 99. 3%(RSD=1. 76%), 100. 5%(RSD=1. 48%) and 99. 2%(RSD=1. 45%)(n=6), respectively. Conclusion:The method shows short analyt-ic time, good stability and promising operation accuracy, which provides the reference for the quality control of Radix Hedysari.
