CAJ | 학술논문

目的：：探讨生肌化瘀方不同溶剂提取部位对伤口愈合的影响,阐明其在深II度烧伤创面愈合过程中所起的重要作用。方法：大鼠随机分成6组(每组10只)：空白组(生理盐水组)、阳性对照组(积雪草苷组)、生肌化瘀方石油醚、氯仿、乙酸乙酯和正丁醇提取物组。将生肌化瘀方不同溶剂提取部位应用于大鼠背部深Ⅱ度烫伤模型,通过不同时间点创面愈合情况、计算机图像分析,活检标本的细胞DNA周期分析及创面组织病理切片等方法观察了上皮愈合的情况。结果：生肌化瘀方氯仿部位创面平均愈合时间为(15.67±1.12)d,空白组为(22.87±1.01)d(P<0.01)；氯仿部位创面羟脯氨酸及S期细胞百分比明显高于对照组(P<0.01)。结论：生肌化瘀方氯仿提取部位有明显的促进创面愈合的作用,为主要活性部位。
목적：：탐토생기화어방불동용제제취부위대상구유합적영향,천명기재심II도소상창면유합과정중소기적중요작용。방법：대서수궤분성6조(매조10지)：공백조(생리염수조)、양성대조조(적설초감조)、생기화어방석유미、록방、을산을지화정정순제취물조。장생기화어방불동용제제취부위응용우대서배부심Ⅱ도탕상모형,통과불동시간점창면유합정황、계산궤도상분석,활검표본적세포DNA주기분석급창면조직병리절편등방법관찰료상피유합적정황。결과：생기화어방록방부위창면평균유합시간위(15.67±1.12)d,공백조위(22.87±1.01)d(P<0.01)；록방부위창면간포안산급S기세포백분비명현고우대조조(P<0.01)。결론：생기화어방록방제취부위유명현적촉진창면유합적작용,위주요활성부위。
Objective:To study the wound healing effects of the extracts of Shengji Huayu Fang with different solvents and demon-strate their important role in wound healing for deep second degree burn wound. Methods:Sixty Sprague-Dawley rats were randomly di-vidied into six groups(ten rats in each group): the control group (saline), the positive control group (asiaticoside group), Shengji Huayu Fang group respectively with petroleum ether, chloroform, ethyl acetate and n-butanol as the extraction solvent. The extracts of Shengji Huayu Fang with different solvents were applied in the rats with deep second degree burn wound, and the wound healing was e-valuated by the healing rate judged by naked eyes and computer image analysis, DNA cell cycle analysis using a flow cytometry, patho-logical reports and the degree of re-epithelialization studied by the other methods. Results:The mean healing time of the chloroform ex-tracts group [(15. 67 ± 1. 12)d] was much shorter than that of the control group [(22. 87 ± 1. 01)d, P<0. 01]. The hydroxyproline content and the percentage of S-phase cells in wound tissue in the chloroform extracts group was obviously higher than those in the con-trol group (P<0. 01). Conclusion:The present study indicates that topical application of chloroform extracts of Shengji Huayu Fang is beneficial to burn wound healing, and the chloroform extracts of Shengji Huayu Fang is the main bioactive fraction.