中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
1期
14-17
,共4页
盐酸罗哌卡因%多囊脂质体%含量%包封率%高效液相色谱法
鹽痠囉哌卡因%多囊脂質體%含量%包封率%高效液相色譜法
염산라고잡인%다낭지질체%함량%포봉솔%고효액상색보법
Ropivacaine hydrochloride%Multivesicular liposomes%Content%Entrapment efficiency%HPLC
目的：：建立盐酸罗哌卡因多囊脂质体中药物含量及包封率的测定方法。方法：采用低速离心法分离游离药物和多囊脂质体,采用高效液相色谱法检测多囊脂质体中游离药物含量和总药量,并计算包封率。结果：盐酸罗哌卡因在1.0~80.0μg·ml-1范围内线性关系良好(r=0.9998)；平均回收率为99.95%,RSD为0.72%(n=9)。用此方法测定3批盐酸罗哌卡因多囊脂质体的主药含量和包封率,结果分别在99.1%~100.3%及80.06%~82.14%之间。结论：该方法操作简单,结果准确,适合用于盐酸罗哌卡因多囊脂质体含量和包封率测定。
目的：：建立鹽痠囉哌卡因多囊脂質體中藥物含量及包封率的測定方法。方法：採用低速離心法分離遊離藥物和多囊脂質體,採用高效液相色譜法檢測多囊脂質體中遊離藥物含量和總藥量,併計算包封率。結果：鹽痠囉哌卡因在1.0~80.0μg·ml-1範圍內線性關繫良好(r=0.9998)；平均迴收率為99.95%,RSD為0.72%(n=9)。用此方法測定3批鹽痠囉哌卡因多囊脂質體的主藥含量和包封率,結果分彆在99.1%~100.3%及80.06%~82.14%之間。結論：該方法操作簡單,結果準確,適閤用于鹽痠囉哌卡因多囊脂質體含量和包封率測定。
목적：：건립염산라고잡인다낭지질체중약물함량급포봉솔적측정방법。방법：채용저속리심법분리유리약물화다낭지질체,채용고효액상색보법검측다낭지질체중유리약물함량화총약량,병계산포봉솔。결과：염산라고잡인재1.0~80.0μg·ml-1범위내선성관계량호(r=0.9998)；평균회수솔위99.95%,RSD위0.72%(n=9)。용차방법측정3비염산라고잡인다낭지질체적주약함량화포봉솔,결과분별재99.1%~100.3%급80.06%~82.14%지간。결론：해방법조작간단,결과준학,괄합용우염산라고잡인다낭지질체함량화포봉솔측정。
Objective:To establish a determination method for the content and entrapment efficiency of ropivacaine hydrochloride-loaded multivesicular liposomes. Methods: The separation of the multivesicular liposomes from the free drug was achieved by low-speed centrifugation. The concentration of ropivacaine hydrochloride in the supernatant and the multivesicular liposomes was determined by HPLC, and the entrapment efficiency was calculated. Results: The linear range of ropivacaine hydrochloride was 1. 0-80. 0μg· ml-1(r=0. 999 8). The average recovery was 99. 95% and RSD was 0. 72%(n=9). The content and entrapment efficiency of three batches of ropivacaine hydrochloride-loaded multivesicular liposomes was within the range of 99. 1%-100. 3% and 80. 06%-82. 14%, respectively. Conclusion:The method is simple and accurate, and can be used in the determination of content and entrapment efficien-cy of ropivacaine hydrochloride-loaded multivesicular liposomes.
