CAJ | 학술논문

目的：测定杜仲药材中5个成分的血浆蛋白结合率。方法采用平衡透析法测定血浆蛋白结合率，生物样本用甲醇沉淀蛋白进行处理，以葛根素为内标，利用超高效液相色谱-串联质谱仪测定血浆和缓冲溶液中的5个成分浓度。结果在所研究浓度范围内，京尼平苷酸、原儿茶酸、绿原酸、松脂醇二葡萄糖苷和松脂醇单葡萄糖苷的平均蛋白结合率分别为(25．77±2．68)%、(57．54±3．79)%、(53．91±3．00)%、(24．15±4．92)%、(49．78±3．61)%。结论京尼平苷酸和松脂醇二葡萄糖苷的蛋白结合率较低，原儿茶酸、绿原酸和松脂醇单葡萄糖苷则与大鼠血浆蛋白有中等强度的结合。
목적：측정두중약재중5개성분적혈장단백결합솔。방법채용평형투석법측정혈장단백결합솔，생물양본용갑순침정단백진행처리，이갈근소위내표，이용초고효액상색보-천련질보의측정혈장화완충용액중적5개성분농도。결과재소연구농도범위내，경니평감산、원인다산、록원산、송지순이포도당감화송지순단포도당감적평균단백결합솔분별위(25．77±2．68)%、(57．54±3．79)%、(53．91±3．00)%、(24．15±4．92)%、(49．78±3．61)%。결론경니평감산화송지순이포도당감적단백결합솔교저，원인다산、록원산화송지순단포도당감칙여대서혈장단백유중등강도적결합。
Aim To determine the plasma protein binding rate of five components of Eucommia ulmoides extract. Methods The equilibrium dialysis method was used to study the plasma protein binding rate. The plasma samples were extracted by protein precipitation with methanol. With the use of puerarin as the internal standard, UPLC-MS/MS was carried out to determine the concentration of the five compounds in and out of the dialysis membrane. Results The average plasma protein binding rates of five compounds on the area of the concentration which was determinate were as fol-lows, respectively: geniposidic acid was ( 25. 77 ± 2. 68 )%, protocatechuic acid was ( 57. 54 ± 3. 79)%, chlorogenic acid was (53. 91 ± 3. 00)%, pinoresinol diglucoside was (24. 15 ± 4. 92)%, and pinoresinol singleglucoside was (49. 78 ± 3. 61)%. Conclusions The results show that the binding percentage of geniposidic acid and pinoresinol diglucoside is relatively low, but the binding rate of the others with rat plasma protein is moderate.