CAJ | 학술논문

目的：研究白藜芦醇预处理对体外氧糖剥夺/再复氧损伤大鼠皮质神经干细胞增殖的影响。方法采用悬浮培养法分离纯化新生SD大鼠大脑皮质神经干细胞。第3代贴壁培养神经干细胞氧糖剥夺150 min后,复氧培养24 h。实验分为正常组、对照组、乙醇组和不同浓度白藜芦醇预处理组。免疫荧光法鉴定细胞,CCK-8法检测细胞活力,流式细胞周期及BrdU法检测细胞增殖。结果悬浮及贴壁培养细胞均高表达巢蛋白( nestin)。与对照组和乙醇组相比,不同浓度白藜芦醇预处理组(1、5、20μmol·L-1)均能明显增强细胞活力,促进细胞增殖,其中以5μmol·L-1白藜芦醇组作用最强( P<0．05)。结论白藜芦醇预处理能减轻氧糖剥夺/再复氧对神经干细胞的损伤,并促进其增殖。
목적：연구백려호순예처리대체외양당박탈/재복양손상대서피질신경간세포증식적영향。방법채용현부배양법분리순화신생SD대서대뇌피질신경간세포。제3대첩벽배양신경간세포양당박탈150 min후,복양배양24 h。실험분위정상조、대조조、을순조화불동농도백려호순예처리조。면역형광법감정세포,CCK-8법검측세포활력,류식세포주기급BrdU법검측세포증식。결과현부급첩벽배양세포균고표체소단백( nestin)。여대조조화을순조상비,불동농도백려호순예처리조(1、5、20μmol·L-1)균능명현증강세포활력,촉진세포증식,기중이5μmol·L-1백려호순조작용최강( P<0．05)。결론백려호순예처리능감경양당박탈/재복양대신경간세포적손상,병촉진기증식。
Aim To study the proliferative effect of resveratrol pretreatment on oxygen-glucose deprivation/reoxygenation ( OGD/R ) injury of rat cortical neural stem cells ( NSCs ) in vitro. Methods Isolation and purification of NSCs in neonatal Sprague-Dawley( SD) rats were conducted by suspended cultivation. The third passage NSCs of adherent culture was cultured under oxygen and glucose deprivation for 150 min and reoxygenation for 24 h. The experimental subjects were divided into normal, control, ethanol and resveratrol pretreatment groups. Immunofluorescence was used to identify NSCs. Cell viability was detected with CCK-8 assay. Flow cytometry cell cycle and BrdU assay were used to measure cell proliferation. Results Cells both in suspended and adherent cultivation highly expressed neuroepithelial stem cell protein ( nestin ) . Compared with the control group, NSCs viabilities and prolifera-tion in resveratrol groups (1, 5, 20 μmol·L-1 ) were significantly heightened, and highest in the 5 μmol · L-1 resveratrol group ( P<0. 05 ) . Conclusion Res-veratrol pretreatment can reduce injury and promote proliferation of NSCs after oxygen-glucose deprivation /reoxygenation.