中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2015年
1期
82-86
,共5页
李昀%张军航%安军%何锦园%黄邵洪
李昀%張軍航%安軍%何錦園%黃邵洪
리윤%장군항%안군%하금완%황소홍
食管癌%磷脂酶Cε1%p53%细胞凋亡%小干扰RNA%表观遗传学
食管癌%燐脂酶Cε1%p53%細胞凋亡%小榦擾RNA%錶觀遺傳學
식관암%린지매Cε1%p53%세포조망%소간우RNA%표관유전학
esophageal cancer%phospholipase cepsi-lon 1%p53%apoptosis%small interfering RNA%epig-enomics
目的：探讨磷脂酶Cε1( PLCE1)基因抑制食管癌细胞凋亡的作用机制。方法选用人食管癌细胞株OE33和CP-C作为研究对象。采用qRT-PCR、Western blot法分别检测转染PLCE1 siRNA前、后食管癌细胞OE33和CP-C中PLCE1、p53 mRNA和蛋白水平的表达；甲基化特异性PCR重亚硫酸盐法检测p53启动子区甲基化状态；流式细胞仪检测细胞凋亡。结果食管癌细胞株OE33和CP-C均高表达PLCE1,抑制PLCE1表达后食管癌细胞CP-C中p53表达上调并促进p53去甲基化,细胞凋亡明显增加。结论 PLCE1通过促进p53启动子区甲基化抑制p53的表达,从而抑制食管癌细胞凋亡。
目的：探討燐脂酶Cε1( PLCE1)基因抑製食管癌細胞凋亡的作用機製。方法選用人食管癌細胞株OE33和CP-C作為研究對象。採用qRT-PCR、Western blot法分彆檢測轉染PLCE1 siRNA前、後食管癌細胞OE33和CP-C中PLCE1、p53 mRNA和蛋白水平的錶達；甲基化特異性PCR重亞硫痠鹽法檢測p53啟動子區甲基化狀態；流式細胞儀檢測細胞凋亡。結果食管癌細胞株OE33和CP-C均高錶達PLCE1,抑製PLCE1錶達後食管癌細胞CP-C中p53錶達上調併促進p53去甲基化,細胞凋亡明顯增加。結論 PLCE1通過促進p53啟動子區甲基化抑製p53的錶達,從而抑製食管癌細胞凋亡。
목적：탐토린지매Cε1( PLCE1)기인억제식관암세포조망적작용궤제。방법선용인식관암세포주OE33화CP-C작위연구대상。채용qRT-PCR、Western blot법분별검측전염PLCE1 siRNA전、후식관암세포OE33화CP-C중PLCE1、p53 mRNA화단백수평적표체；갑기화특이성PCR중아류산염법검측p53계동자구갑기화상태；류식세포의검측세포조망。결과식관암세포주OE33화CP-C균고표체PLCE1,억제PLCE1표체후식관암세포CP-C중p53표체상조병촉진p53거갑기화,세포조망명현증가。결론 PLCE1통과촉진p53계동자구갑기화억제p53적표체,종이억제식관암세포조망。
Aim To investigate the role of phospho-lipase C epsilon 1 ( PLCE1 ) in modulating the apoptot-ic mechanism in esophageal cancer ( Eca ) cells. Methods Eca cell lines, OE33 and CP-C cells were cultured to assess the expression of PLCE1 . siRNA suppress expression of PLCE1 . The expression of PLCE1 and p53 was evaluated by quantitative real time PCR and Western blot. Methylation analyses of p53 were performed by bisulfite conversion of genomic DNA. Apoptosis was assessed by flow cytometry. Results OE33 and CP-C cells expressed high levels of PLCE1 . Knockdown of PLCE1 markedly increased the expression and hypomethylation of p53 , and in-creased the frequency of apoptosis. Conclusion PLCE1 suppresses apoptosis of Eca cells via promoting p53 promoter methylation and inhibiting expression of p53 .
