CAJ | 학술논문

目的：在大鼠侧脑室内给予不同剂量的瞬时受体电位香草酸受体1(transient receptor potential vanilloid 1,TRPV1)拮抗剂Capsazepine,观察对LPS致热过程的影响。方法在侧脑室内预先给与3种剂量Capsazepine,随之腹腔给与LPS致热。在腹腔内埋植发射子遥测体温变化过程,同时应用荧光测定法检测下丘脑细胞内钙离子浓度的变化,Western blot方法检测TRPV1表达水平。结果随着Capsazepine剂量增大,LPS致大鼠发热水平升高,下丘脑细胞内钙离子浓度减少,TRPV1表达水平降低。结论 Capsazepine作用于大鼠下丘脑,可使LPS诱导的发热水平提高,此效应可能与TR-PV1的作用有关。
목적：재대서측뇌실내급여불동제량적순시수체전위향초산수체1(transient receptor potential vanilloid 1,TRPV1)길항제Capsazepine,관찰대LPS치열과정적영향。방법재측뇌실내예선급여3충제량Capsazepine,수지복강급여LPS치열。재복강내매식발사자요측체온변화과정,동시응용형광측정법검측하구뇌세포내개리자농도적변화,Western blot방법검측TRPV1표체수평。결과수착Capsazepine제량증대,LPS치대서발열수평승고,하구뇌세포내개리자농도감소,TRPV1표체수평강저。결론 Capsazepine작용우대서하구뇌,가사LPS유도적발열수평제고,차효응가능여TR-PV1적작용유관。
Aim To observe the effects of the transient receptor potential vanilloid receptor 1 ( TRPV1 ) antago-nist Capsazepine on the LPS-induced fever process. Methods Three doses of Capsazepine were respec-tively injected into rats’ lateral ventricles before the in-traperitoneal injection of LPS caused fever. An emitter was implanted inside the rat’ s abdominal cavity to mo-nitor the body temperature changes during the fever process. Then the changes of calcium ion in the hypo-thalamus were detected by fluorescence and the expres-sions of TRPV1 protein were detected by Western blot. Results The higher dosage of Capsazepine was injec-ted, the higher the level of LPS-induced fever was, and the lower the concentration of calcium ion in the hypothalamus as well as the TRPV1 expressions were. Conclusion Capsazepine could increase the level of fever induced by LPS via acting on the rats’ hypothala-mus. This effect might be related to the functions of TRPV1 .