CAJ | 학술논문

以产酸性蛋白酶较高的菌株Y-9及F-5为出发菌株，对它们进行基因组改组，进行原生质体双灭活，以PEG作为融合剂，并对融合条件进行优化。试验表明：融合最佳条件为PEG 50％、p H 6．0、融合处理15 min，此时融合率达到12％。通过第一及第二轮的基因组改组，最终得到产酸性蛋白酶较出发菌株Y-9高出了253．37％，较F-5高出了276．25％的米曲霉菌株G11。
이산산성단백매교고적균주Y-9급F-5위출발균주，대타문진행기인조개조，진행원생질체쌍멸활，이PEG작위융합제，병대융합조건진행우화。시험표명：융합최가조건위PEG 50％、p H 6．0、융합처리15 min，차시융합솔체도12％。통과제일급제이륜적기인조개조，최종득도산산성단백매교출발균주Y-9고출료253．37％，교F-5고출료276．25％적미곡매균주G11。
In this paper,strain Y-9 and F-5 are used as the starting strains for genome shuffling.They are inactivated by UV irradiation and heating. The technology of PEG is applied to mediate protoplasts fusion.The optimization of fusion conditions is studied.The optimal conditions for fusion are that PEG 50%,pH 6.0,fusion for 15 min,the fusion rate reaches 12%.After two rounds of genome shuffling,Aspergillus oryzae G11 is obtained finally that produces acid protease higher than the starting strain Y-9 and F-5 by 253.37% and 276.25% respectively.