现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2015年
1期
1-4
,共4页
王帆%周戬平%谢小娟%胡军%郭华%胡巧侠
王帆%週戩平%謝小娟%鬍軍%郭華%鬍巧俠
왕범%주전평%사소연%호군%곽화%호교협
长链非编码RNA%UCA1%卵巢癌%肿瘤侵袭%肿瘤转移
長鏈非編碼RNA%UCA1%卵巢癌%腫瘤侵襲%腫瘤轉移
장련비편마RNA%UCA1%란소암%종류침습%종류전이
lncRNA%UCA1%ovarian cancer%tumor invasion%tumor metastasis
目的:探讨长链非编码RNA UCA1对卵巢癌细胞SKOV3侵袭及迁移能力的影响及可能的作用机制。方法:用脂质体2000将pcDNA/UCA1表达载体及pcDNA3.1空载体转染卵巢癌细胞SKOV3细胞。用G418筛选,建立稳定表达UCA1 RNA的SKOV3/pcDNA-UCA1细胞及转染空载体的SKOV3/pcDNA3.1细胞。RT-PCR方法检测两株细胞中UCA1的表达,鉴定阳性细胞株。Millicell小室检测两株细胞侵袭及迁移能力的变化,蛋白印迹法检测两株细胞MMP2及MMP9蛋白表达的变化。结果:成功构建稳定表达UCA1的SKOV3细胞,表达UCA1 RNA后,SKOV3细胞的侵袭及迁移能力均增加,MMP2及MMP9蛋白表达增加。结论:UCA1 RNA可能通过增加SKOV3细胞中MMP2及MMP9的表达来增强其侵袭及迁移能力,在卵巢癌侵袭及进展中发挥作用。
目的:探討長鏈非編碼RNA UCA1對卵巢癌細胞SKOV3侵襲及遷移能力的影響及可能的作用機製。方法:用脂質體2000將pcDNA/UCA1錶達載體及pcDNA3.1空載體轉染卵巢癌細胞SKOV3細胞。用G418篩選,建立穩定錶達UCA1 RNA的SKOV3/pcDNA-UCA1細胞及轉染空載體的SKOV3/pcDNA3.1細胞。RT-PCR方法檢測兩株細胞中UCA1的錶達,鑒定暘性細胞株。Millicell小室檢測兩株細胞侵襲及遷移能力的變化,蛋白印跡法檢測兩株細胞MMP2及MMP9蛋白錶達的變化。結果:成功構建穩定錶達UCA1的SKOV3細胞,錶達UCA1 RNA後,SKOV3細胞的侵襲及遷移能力均增加,MMP2及MMP9蛋白錶達增加。結論:UCA1 RNA可能通過增加SKOV3細胞中MMP2及MMP9的錶達來增彊其侵襲及遷移能力,在卵巢癌侵襲及進展中髮揮作用。
목적:탐토장련비편마RNA UCA1대란소암세포SKOV3침습급천이능력적영향급가능적작용궤제。방법:용지질체2000장pcDNA/UCA1표체재체급pcDNA3.1공재체전염란소암세포SKOV3세포。용G418사선,건립은정표체UCA1 RNA적SKOV3/pcDNA-UCA1세포급전염공재체적SKOV3/pcDNA3.1세포。RT-PCR방법검측량주세포중UCA1적표체,감정양성세포주。Millicell소실검측량주세포침습급천이능력적변화,단백인적법검측량주세포MMP2급MMP9단백표체적변화。결과:성공구건은정표체UCA1적SKOV3세포,표체UCA1 RNA후,SKOV3세포적침습급천이능력균증가,MMP2급MMP9단백표체증가。결론:UCA1 RNA가능통과증가SKOV3세포중MMP2급MMP9적표체래증강기침습급천이능력,재란소암침습급진전중발휘작용。
Objective:To study the invasion and migration ability of long chain non-coding RNA UCA1 in ovari-an cancer cells SKOV3. Methods:Constructing eukaryotic expression vector pcDNA/UCA1. pcDNA/UCA1 construct was transfected into SKOV3 cells by lipofectamine2000 for 24h and selected with 500μg/ml G418 for 3 weeks,trans-fected with pcDNA3. 1 empty vector acted as a control. The positive clone was identified by reverse transcription poly-merase chain reaction( RT -PCR ) for UCA 1 RNA expression . Cell invasion and migration ability was assessed by using millicell chamber. MMP2 and MMP9 protein level was detected by Western blotting analysis. Results:We successfully established the SKOV3 cell stable expressing pcDNA/UCA1 transfectant. Exogenous expression of UCA1 in SKOV3 cells enhanced migration and invasion ability of cells. Conclusion:MMP2 and MMP9 protein expression in-creased in SKOV3/pcDNA-UCA1 cells compared with SKOV3/pcDNA3. 1 cells. Which suggested that UCA1 RNA might play some role in SKOV3 cells invasion and progression.