CAJ | 학술논문

目的：血液回输能够迅速增加机体红细胞数，增强有氧运动能力，提高运动成绩。采用流式细胞技术对异体血液回输的检测方法进行适用性验证研究，以证明该技术检测异体血液回输的可靠性和可操作性。研究方法：应用流式细胞技术，通过检测血液中荧光标记的红细胞血型抗原表达形式的方法，追踪是否存在微量的异体红细胞群，并判断是否接受异体血液回输。3名检测员使用流式细胞仪对46例不同比例（0～5％）体外混合的全血进行8种红细胞血型抗原的检测。结果：本研究对该方法的特异性、灵敏度、精密度、稳定性等进行评估。此方法检测无假阳性结果；可准确检测不低于3％的异体输血量，灵敏度较高；经细胞稳定液处理4～8℃储存的样品在4周内重复检测结果一致，3名检测员检测的结果一致。结论：流式细胞技术检测异体血液回输的方法准确可靠，符合实验室国际标准（ISO‐17025）认证和验证要求，已通过中国合格评定认可委员会认可，并应用于常规和奥运会等大型赛事的异体血液回输兴奋剂检测。
목적：혈액회수능구신속증가궤체홍세포수，증강유양운동능력，제고운동성적。채용류식세포기술대이체혈액회수적검측방법진행괄용성험증연구，이증명해기술검측이체혈액회수적가고성화가조작성。연구방법：응용류식세포기술，통과검측혈액중형광표기적홍세포혈형항원표체형식적방법，추종시부존재미량적이체홍세포군，병판단시부접수이체혈액회수。3명검측원사용류식세포의대46례불동비례（0～5％）체외혼합적전혈진행8충홍세포혈형항원적검측。결과：본연구대해방법적특이성、령민도、정밀도、은정성등진행평고。차방법검측무가양성결과；가준학검측불저우3％적이체수혈량，령민도교고；경세포은정액처리4～8℃저존적양품재4주내중복검측결과일치，3명검측원검측적결과일치。결론：류식세포기술검측이체혈액회수적방법준학가고，부합실험실국제표준（ISO‐17025）인증화험증요구，이통과중국합격평정인가위원회인가，병응용우상규화오운회등대형새사적이체혈액회수흥강제검측。
Objective :Blood transfusion could increase red blood cells (RBCs ) rapidly and may enhance endurance performance significantly .A validation was carried out to prove the reliabili‐ty and operability of the detection of homologous blood transfusion with flow cytometry tech‐nology .Method :Homologous blood transfusion was detected using flow cytometry technique to observe the presence of different RBCs phenotypes of eight different antigens in a blood sam‐ple .The analysis of 46 blood samples containing different percentages (0 ~ 5% ) of homolo‐gous RBCs was carried out by three independent analysts .Results :The method afforded satis‐factory results in terms of robustness ,sensitivity ,specificity ,precision and stability .No false positive results were observed .All samples contained 3% homologous RBCs were unambigu‐ously detected .These samples were stable over 4 weeks after using cell stable buffer at 4 ~8℃ .Conclusion :This method fulfils the ISO‐17025 accreditation and has been accredited by China National Accreditation Service for Conformity Assessment (CNAS ) .The method has been successfully utilized to the detection of homologous blood transfusion in major events and routine doping‐control samples .