现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2015年
1期
19-22
,共4页
刘丽娟%关国锋%王小娟%薛琳%李亚妮%王飙落%梁树辉%丁杰
劉麗娟%關國鋒%王小娟%薛琳%李亞妮%王飆落%樑樹輝%丁傑
류려연%관국봉%왕소연%설림%리아니%왕표락%량수휘%정걸
AMD3100%CXCR4%胃癌%增殖%侵袭
AMD3100%CXCR4%胃癌%增殖%侵襲
AMD3100%CXCR4%위암%증식%침습
AMD3100%CXCR4%gastric cancer%proliferation%invasion
目的:探讨CXCR4特异性非肽类受体拮抗剂AMD3100对胃癌细胞增殖和侵袭能力的影响及其可能的分子机制。方法:Western blot方法检测不同转移潜能胃癌细胞系中CXCR4蛋白的表达水平。以不同浓度的AMD3100处理MKN28-M和MKN28-NM细胞后,采用CCK-8检测胃癌细胞的增殖情况,Transwell实验观察胃癌细胞侵袭能力的变化。Western blot检测AMD3100处理后MKN28-M细胞中基质金属蛋白酶-9(MMP-9)、血管内皮生长因子(VEGF)的表达。结果:Western blot实验结果显示,高转移胃癌细胞MKN28-M中CXCR4的蛋白表达水平明显高于低转移胃癌细胞MKN28-NM,亲本细胞MKN28的CXCR4蛋白表达量很低。CCK-8和 Transwell 实验检测结果表明,AMD3100通过特异性阻断 CXCR4的作用,显著抑制MKN28-M和MKN28-NM细胞的增殖能力( p<0.05)和侵袭能力( p<0.01),且其抑制均呈剂量依赖性。经AMD3100处理后,MKN28-M细胞中MMP-9和VEGF的蛋白表达水平降低。结论:AMD3100可能通过下调MMP-9、VEGF等分子的表达抑制胃癌细胞的增殖,减弱胃癌细胞的侵袭及转移能力。
目的:探討CXCR4特異性非肽類受體拮抗劑AMD3100對胃癌細胞增殖和侵襲能力的影響及其可能的分子機製。方法:Western blot方法檢測不同轉移潛能胃癌細胞繫中CXCR4蛋白的錶達水平。以不同濃度的AMD3100處理MKN28-M和MKN28-NM細胞後,採用CCK-8檢測胃癌細胞的增殖情況,Transwell實驗觀察胃癌細胞侵襲能力的變化。Western blot檢測AMD3100處理後MKN28-M細胞中基質金屬蛋白酶-9(MMP-9)、血管內皮生長因子(VEGF)的錶達。結果:Western blot實驗結果顯示,高轉移胃癌細胞MKN28-M中CXCR4的蛋白錶達水平明顯高于低轉移胃癌細胞MKN28-NM,親本細胞MKN28的CXCR4蛋白錶達量很低。CCK-8和 Transwell 實驗檢測結果錶明,AMD3100通過特異性阻斷 CXCR4的作用,顯著抑製MKN28-M和MKN28-NM細胞的增殖能力( p<0.05)和侵襲能力( p<0.01),且其抑製均呈劑量依賴性。經AMD3100處理後,MKN28-M細胞中MMP-9和VEGF的蛋白錶達水平降低。結論:AMD3100可能通過下調MMP-9、VEGF等分子的錶達抑製胃癌細胞的增殖,減弱胃癌細胞的侵襲及轉移能力。
목적:탐토CXCR4특이성비태류수체길항제AMD3100대위암세포증식화침습능력적영향급기가능적분자궤제。방법:Western blot방법검측불동전이잠능위암세포계중CXCR4단백적표체수평。이불동농도적AMD3100처리MKN28-M화MKN28-NM세포후,채용CCK-8검측위암세포적증식정황,Transwell실험관찰위암세포침습능력적변화。Western blot검측AMD3100처리후MKN28-M세포중기질금속단백매-9(MMP-9)、혈관내피생장인자(VEGF)적표체。결과:Western blot실험결과현시,고전이위암세포MKN28-M중CXCR4적단백표체수평명현고우저전이위암세포MKN28-NM,친본세포MKN28적CXCR4단백표체량흔저。CCK-8화 Transwell 실험검측결과표명,AMD3100통과특이성조단 CXCR4적작용,현저억제MKN28-M화MKN28-NM세포적증식능력( p<0.05)화침습능력( p<0.01),차기억제균정제량의뢰성。경AMD3100처리후,MKN28-M세포중MMP-9화VEGF적단백표체수평강저。결론:AMD3100가능통과하조MMP-9、VEGF등분자적표체억제위암세포적증식,감약위암세포적침습급전이능력。
Objective:To explore the effect of AMD3100,which is the specific inhibitor of CXCR4,on proliferation and invasion of gastric cancer cells and its probable mechanism. Methods:Western blot was used to detect the expres-sion of CXC chemokine receptor-4(CXCR4)in different metastatic potential cell lines. AMD3100 at different final concentrations was applied to treat with human gastric cancer cell line MKN28-M and MKN28-NM. CCK-8 assay was used to detect their proliferation. Transwell assay was used to identify the invasive ability in MKN28 -M and MKN28-NM cell lines. Western blot was employed to detect the expressions of matrix metalloproteinase-9( MMP-9)and vascular endothelial growth factor(VEGF)in MKN28-M cell line after incubation with AMD3100. Results:Western blot showed that expression level of CXCR4 in MKN28-M cells was higher than MKN28-NM,while with hardly expression in MKN28 cells. The proliferation of MKN28-M and MKN28-NM were significantly suppressed by AMD3100 in a dose-dependent manner(p<0. 05). AMD3100(100 and 1000ng/ml)significantly inhibited the invasion ability of MKN28-M and MKN28-NM cells(p<0. 01). Treatment with AMD3100 markedly reduced the expression of MMP-9 and VEGF in MKN28-M cells . Conclusion:AMD 3 1 0 0 inhibits proliferation and invasion activities of gastric cells may through down-regulation of MMP-9 and VEGF expression.