CAJ | 학술논문

目的：探讨糖尿病性痛（Diabetic neuropathic pain ，DNP）模型大鼠中前列腺酸性磷酸酶（Prostatic acid phosphatase ，PAP）的时空表达特点。方法：采用链脲佐菌素（Streptozocin ， STZ）腹腔注射诱导建立DNP大鼠模型，应用Von‐Frey细丝法和 Hargreaves热辐射法观察大鼠的机械痛和热痛变化。免疫组化法检测腰膨大节段（L4～5）脊髓背角（Spinal dorsal horn ，SDH）和背根神经节（Dorsal root ganglion ，DRG）中PAP随DNP病程发展的表达。结果：DNP大鼠的机械痛阈和热痛阈在造模后第7天开始明显降低，并维持至第35天，与对照组相比有显著性差异（ P＜0．05）；造模7 d后模型组大鼠DRG神经元中PAP表达明显减少，14 d和21 d时表达进一步降低，并持续至35 d；而模型组大鼠SD H中PA P的表达从造模后14 d开始逐渐减少至35 d ，但PA P表达在SDH中的变化较DRG晚1周。结论：随着DNP大鼠病程进展，PAP在DRG和SDH中的表达均呈现减少的趋势，且与痛敏变化几乎平行；提示PAP可能是DNP中一种具有调控镇痛效应的分子。
목적：탐토당뇨병성통（Diabetic neuropathic pain ，DNP）모형대서중전렬선산성린산매（Prostatic acid phosphatase ，PAP）적시공표체특점。방법：채용련뇨좌균소（Streptozocin ， STZ）복강주사유도건립DNP대서모형，응용Von‐Frey세사법화 Hargreaves열복사법관찰대서적궤계통화열통변화。면역조화법검측요팽대절단（L4～5）척수배각（Spinal dorsal horn ，SDH）화배근신경절（Dorsal root ganglion ，DRG）중PAP수DNP병정발전적표체。결과：DNP대서적궤계통역화열통역재조모후제7천개시명현강저，병유지지제35천，여대조조상비유현저성차이（ P＜0．05）；조모7 d후모형조대서DRG신경원중PAP표체명현감소，14 d화21 d시표체진일보강저，병지속지35 d；이모형조대서SD H중PA P적표체종조모후14 d개시축점감소지35 d ，단PA P표체재SDH중적변화교DRG만1주。결론：수착DNP대서병정진전，PAP재DRG화SDH중적표체균정현감소적추세，차여통민변화궤호평행；제시PAP가능시DNP중일충구유조공진통효응적분자。
Objective:To investigate the spatio‐temporal expression of prostatic acid phosphatase (PAP) in rats with diabetic neuropathic pain (DNP) .Methods :DNP rat model was induced by intraperitoneal (ip) injection of streptozocin (STZ) .The Von‐Frey filaments and Hargreaves tests were applied to identify the changes of the paw withdrawal threshold (PWT ) and paw withdrawal latency (PWL ) in DNP rats .Immunohistochemistry was em‐ployed to detect the spatio‐temporal expression of PAP in the spinal dorsal horn (SDH ) and dorsal root ganglion (DRG) on different time points after i .p .injection of STZ ,respectively .Results :Compared with control group ,i . p .injection of STZ induced significant mechanical allodynia and thermal hyperalgesia indicated by the reduced PWT and PWL from 7 d ,and maintained to 35 d (P < 0 .05) .The expression of PAP in DRG neurons was significantly decreased from 7 d to 21 d (P < 0 .05) ,then slightly increased from 28 d to 35 d;while that in SDH was markedly decreased from 14 d to 35 d (P < 0 .05) ,compared with control group ,but the changes in SDH were 1 w later than that in DRG ..Conclusion:With the progress of DNP ,the expression of PAP was significantly decreased in the DRG and SDH ,which was almost parallel with the pain process .These results have suggested that PAP may play a key role in the alleviation pain of the DNP .