CAJ | 학술논문

目的：探讨不同生物学特性胃癌中Notch－1基因与p53蛋白、Ki－67表达的意义。方法采用免疫组织化学染色方法检测胃癌及其癌旁组织中的Notch－1、p53、Ki－67的表达，进行相关性分析。结果 Notch－1、p53、Ki－67在胃癌组织中的阳性表达率分别为57．5％（48／80）、47．5％（38／80）、52．5％（42／80），均明显高于癌旁组（分别为28．1％、0％、和12．5％，P＜0．05），并且都与肿瘤分化程度、脉管浸润明显呈正相关（P＜0．05）；p53、Ki－67与淋巴结转移明显相关（P＜0．05），但Notch－1与肿瘤浸润深度、淋巴结转移无明显相关性（P＞0．05）；Notch－1与p53、Ki－67表达均有高度正相关性。结论 Notch－l与p53在胃癌的进展中可能共同起作用，联合检测Notch－1、p53、Ki－67可能成为胃癌患者预后判断重要参考指标。
목적：탐토불동생물학특성위암중Notch－1기인여p53단백、Ki－67표체적의의。방법채용면역조직화학염색방법검측위암급기암방조직중적Notch－1、p53、Ki－67적표체，진행상관성분석。결과 Notch－1、p53、Ki－67재위암조직중적양성표체솔분별위57．5％（48／80）、47．5％（38／80）、52．5％（42／80），균명현고우암방조（분별위28．1％、0％、화12．5％，P＜0．05），병차도여종류분화정도、맥관침윤명현정정상관（P＜0．05）；p53、Ki－67여림파결전이명현상관（P＜0．05），단Notch－1여종류침윤심도、림파결전이무명현상관성（P＞0．05）；Notch－1여p53、Ki－67표체균유고도정상관성。결론 Notch－l여p53재위암적진전중가능공동기작용，연합검측Notch－1、p53、Ki－67가능성위위암환자예후판단중요삼고지표。
Objective To study the expression of Notch-1 in gastric carcinoma and its relationship with the expression of p53 and Ki-67. Methods Immunohistochemical staining was applied to measure the expression of Notch-1,p53 and Ki-67 in the carci?noma tissues and pericarcinomatous tissues. And the correlation among the expression of Notch -1, p53 and Ki -67 was analyzed. Results The positive expression rates of Notch-1,p53 and Ki-67 in gastric carcinoma group were respectively 57. 5% (48/80), 47. 5% (38/80) and 52. 5% (42/80),remarkably higher than those in the pericarcinomatous group (28. 1%,0% and 12. 5% respec?tively,P<0. 05) and were significantly correlated with differentiation grade and vessel invasion (P<0. 05 or P<0. 05). The expression of p53 and Ki-67 was closely correlated with lymph nodes metastasis (P<0. 05). However,Notch-1 expression was not statistically correlated with depth of invasion and lymph nodes metastasis (P>0. 05). The expression of Notch-1 was positively correlated with the expression of p53 and Ki-67. Conclusion The expression of Notch-1 and p53 may play a synergetic role in gastric cancer incidence and development. Combined detection of Notch-1,p53 and Ki-67 might be an important prognostic marker of gastric cancer.