CAJ | 학술논문

目的：观察白三烯B4（LTB4）、白三烯D4（LTD4）、基质金属蛋白酶2（MMP2）在动脉粥样硬化大鼠中的表达，探讨其致动脉粥样硬化的机制。方法选取雄性SD大鼠24只，随机分为正常组和动脉硬化组。正常组给予普通饲料喂养，动脉硬化组给予高脂饲料喂养，14周后建立动脉粥样硬化大鼠模型，处死大鼠，采血，检测血脂，ELISA法检测血清和主动脉中白LTB4、D4、MMP2的含量。结果与正常组比较，动脉硬化组甘油三酯、胆固醇、低密度脂蛋白水平明显升高（P＜0．05），高密度脂蛋白水平减低（P＜0．05）。动脉硬化组主动脉以及血液中LTB4、LTD4、MMP2的含量与正常组比较明显升高（P＜0．05）。结论 LTB4、D4、MMP2在动脉硬化大鼠血液及主动脉中均异常表达，是致动脉粥样硬化重要的炎症因子。
목적：관찰백삼희B4（LTB4）、백삼희D4（LTD4）、기질금속단백매2（MMP2）재동맥죽양경화대서중적표체，탐토기치동맥죽양경화적궤제。방법선취웅성SD대서24지，수궤분위정상조화동맥경화조。정상조급여보통사료위양，동맥경화조급여고지사료위양，14주후건립동맥죽양경화대서모형，처사대서，채혈，검측혈지，ELISA법검측혈청화주동맥중백LTB4、D4、MMP2적함량。결과여정상조비교，동맥경화조감유삼지、담고순、저밀도지단백수평명현승고（P＜0．05），고밀도지단백수평감저（P＜0．05）。동맥경화조주동맥이급혈액중LTB4、LTD4、MMP2적함량여정상조비교명현승고（P＜0．05）。결론 LTB4、D4、MMP2재동맥경화대서혈액급주동맥중균이상표체，시치동맥죽양경화중요적염증인자。
Objective To observe the expression of leukotrieneB4(LTB4), leukotriene D4(LTD4)and Matrix metalloproteinas?es2(MMP2)in atherosclerotic rats and explore the mechanism of atherosclerosis. Methods Twenty-four male SD rats were divided into normal group and atherosclerotic group. Normal group ratswere fed with normal diet,Atherosclerotic group rats were fed withhigh-fat di?et. After 14 weeks, atherosclerosis models were found, thenthe rats were killed and the lipids,LTB4,LTD4 and MMP2 in the blood and aorta were measure by Enzyme-Linked Immunosorbnent Assay. Results Compared with normal group,there was significantly increased in the level of Triglyceride (TG), cholesterol (CHOL), low density lipoprotein (LDL) in atherosclerotic group(P<0. 05)and the level of high-density lipoprotein (HDL) was lesser(P<0. 05). Meanwhile,the expression of LTB4、LTD4、MMP2 in the blood and artor of atherosclerotic rats were significantly higher than those in normal group(P<0. 05). Conclusion The expression of LTB4,LTD4,MMP2 are abnormal in the blood and artor of atherosclerotic rats,which are important inflammatory factors that bring aboutatherosclerosis.