CAJ | 학술논문

为了提高烟草浸膏中的还原糖和氨基酸含量，以红大碎片为原料，以还原糖得率和氨基酸得率为指标，在单因素试验的基础上采用L9(34)正交试验法对酶法提取制备烟草浸膏工艺进行优化。结果表明，酶法提取制备烟草浸膏较优工艺为：同时各添加原料质量0.2%的Viscozyme L复合水解酶、淀粉酶和风味蛋白酶，在40℃下水浴酶解4.0 h后，微沸提取0.5 h。采用该工艺制备得到的红大烟叶浸膏得率较常规提取增加了11.84个百分点，还原糖含量增加了36.46%，氨基酸含量增加了8.08%，致香成分总量增加了8.08%，其中含量增加较明显的美拉德反应产物是2-糠醛缩二乙醇、糠醛、5-甲基糠醛和吡咯，分别增加了204.07%、108.21%、92.28%和42.67%。
위료제고연초침고중적환원당화안기산함량，이홍대쇄편위원료，이환원당득솔화안기산득솔위지표，재단인소시험적기출상채용L9(34)정교시험법대매법제취제비연초침고공예진행우화。결과표명，매법제취제비연초침고교우공예위：동시각첨가원료질량0.2%적Viscozyme L복합수해매、정분매화풍미단백매，재40℃하수욕매해4.0 h후，미비제취0.5 h。채용해공예제비득도적홍대연협침고득솔교상규제취증가료11.84개백분점，환원당함량증가료36.46%，안기산함량증가료8.08%，치향성분총량증가료8.08%，기중함량증가교명현적미랍덕반응산물시2-강철축이을순、강철、5-갑기강철화필각，분별증가료204.07%、108.21%、92.28%화42.67%。
In order to increase the contents of reducing sugar and amino acids which are beneficial to the smoking quality of the tobacco extractum,the enzymatic extraction was studied and optimized. With Hong-da fragments as raw material,the yields of reducing sugar and amino acids as indexes,the L9 (34 ) orthogo-nal experiment was applied to optimize the process. After optimization,the Hongda fragments were hydro-lyzed by 0. 2% of Viscozyme L enzyme,0. 2% of amylase and 0. 2% of flavor protease for 4. 0 h at 40 ℃. Compared with the conventional extraction,the yield of the tobacco extractum prepared by enzy-matic extraction increased by 11. 84 percentage points,the reducing sugar content increased by 36. 46%, the amino acid content increased by 8. 08%,the total content of aroma components increased by 8. 08%, in which Maillard reaction products increased obviously,including 2-furfural diethanolamine,furfural,5-methyl furfural and pyrrole,increased by 204. 07%,108. 21%,92. 28% and 42. 67%,respectively.