CAJ | 학술논문

为了探讨外源黄体酮对高温胁迫下玉米幼苗脂质过氧化及抗氧化酶系统的影响,以玉米驻玉309为材料,人工模拟高温条件下采用不同浓度外源黄体酮处理玉米幼苗,研究其电导率、丙二醛( MDA )含量、光合作用放氧速率、叶绿素荧光参数、超氧化物歧化酶( SOD )活性、过氧化氢酶( CAT)活性、过氧化物酶( POD)活性及CAT、SOD同工酶的变化。结果显示,10-9 mol/L黄体酮处理玉米幼苗CAT活性为高温处理的2.4倍；10-7 mol/L黄体酮处理玉米幼苗SOD、POD 活性分别为高温处理的1.1倍、1.2倍；10-9 mol/L、10-7 mol/L黄体酮处理的幼苗高温下其外渗液相对电导率减少至高温处理的70%和65%,MDA含量减少至高温处理的69%和74%；10-7 mol/L黄体酮处理的玉米幼苗其POD、CAT同工酶表达量和放氧速率明显增加,同时叶绿素荧光参数与对照(常温水处理)植株相比也有明显缓解。研究表明,10-9 mol/L、10-7 mol/L 黄体酮处理可以提高高温胁迫下玉米幼苗抗氧化酶的活性及同工酶的表达,降低膜脂过氧化及电解质外渗率,改善光合作用。
위료탐토외원황체동대고온협박하옥미유묘지질과양화급항양화매계통적영향,이옥미주옥309위재료,인공모의고온조건하채용불동농도외원황체동처리옥미유묘,연구기전도솔、병이철( MDA )함량、광합작용방양속솔、협록소형광삼수、초양화물기화매( SOD )활성、과양화경매( CAT)활성、과양화물매( POD)활성급CAT、SOD동공매적변화。결과현시,10-9 mol/L황체동처리옥미유묘CAT활성위고온처리적2.4배；10-7 mol/L황체동처리옥미유묘SOD、POD 활성분별위고온처리적1.1배、1.2배；10-9 mol/L、10-7 mol/L황체동처리적유묘고온하기외삼액상대전도솔감소지고온처리적70%화65%,MDA함량감소지고온처리적69%화74%；10-7 mol/L황체동처리적옥미유묘기POD、CAT동공매표체량화방양속솔명현증가,동시협록소형광삼수여대조(상온수처리)식주상비야유명현완해。연구표명,10-9 mol/L、10-7 mol/L 황체동처리가이제고고온협박하옥미유묘항양화매적활성급동공매적표체,강저막지과양화급전해질외삼솔,개선광합작용。
To investigate the effect of exogenous progesterone on the activities of lipid peroxidation and antioxidant enzymes,the seedlings of maize (Zea may cv. Zhuyu 309) were pretreated with 10 -9 mol/L, 10 -7 mol/L,10 -5 mol/L,10 -3 mol/L of progesterone under the high temperature stress. The activities of superoxide dismutase ( SOD) ,catalase ( CAT) ,peroxidase ( POD) ,CAT and POD isozymes,and the ma-londialdehyde ( MDA) content,membrane permeability and chlorophyll fluorescence parameters of maize leaves were assayed. The results showed that,CAT activity of 10 -9 mol/L progesterone pretreatment was 2. 4 times higher than the high temperature treatment,and the activities of SOD and POD of 10 -7 mol/L progesterone pretreatment were 1. 1 times and 1. 2 times higher than the high temperature treatment,re-spectively. In addition,the relative electric conductivity of 10 -9 mol/L and 10 -7 mol/L progesterone pre-treatment decreased to 70% and 65% of the high temperature treatment,respectively; Malondialdehyde contents were also reduced to 69% or 74% of the high temperature treatment,respectively. The expression levels of POD and CAT isozymes of 10 -7 mol/L progesterone treatment elevated,as well as oxygen release rate and chlorophyll fluorescence paraments. This study showed that the pretreatment with 10 -9 mol/L and 10 -7 mol/L progesterone increased the activities of antioxidant enzymes,and decreased the MDA content and the electrolyte leakage rate under high temperature stress,thus to protect the photosynthesis in maize leaves.