CAJ | 학술논문

目的：探讨急性白血病五色流式细胞术免疫分型与FAB分型的关系和表达规律。方法通过CD45/SSC设门的五色流式细胞仪检测118例急性白血病患者骨髓细胞的表面抗原，并进行免疫表型分析。结果在88例AML患者中髓系相关抗原的表达率依次为CD33(93.2%)>CD13(88.6%)>CD117(86.4%)，部分AML患者伴表达淋系相关抗原，主要为CD56(25.2%)、CD7(11.3%)、CD19(2.2%)、CD20(2.2%)；M3中高表达CD117(83.7%)、CD13(83.7%)、CD33(83.7%)，但均不表达CD34、HLA-DR，且SSC较大；M4、M5中可见CD64、CD4、CD14明显表达高于AML其他亚型(P<0.01)。19例B-ALL患者中CD19的表达率最高(100%)，其次为CD34(90.1%)、HLA-DR (90.1%)、CD10(90.1%)；9例T-ALL患者中CD7的表达率最高(100%)，其次为CD4(77.8%)、CD5(77.8%)、CD2(66.7%)、CD3(66.7%)、CD34(55.6%)。结论通过五色流式细胞术进行白血病免疫分型是形态学分型诊断重要的补充，能够提供更客观的诊断依据，对提高急性白血病的诊断正确率、预后判断和治疗方案的选择有重要的意义。
목적：탐토급성백혈병오색류식세포술면역분형여FAB분형적관계화표체규률。방법통과CD45/SSC설문적오색류식세포의검측118례급성백혈병환자골수세포적표면항원，병진행면역표형분석。결과재88례AML환자중수계상관항원적표체솔의차위CD33(93.2%)>CD13(88.6%)>CD117(86.4%)，부분AML환자반표체림계상관항원，주요위CD56(25.2%)、CD7(11.3%)、CD19(2.2%)、CD20(2.2%)；M3중고표체CD117(83.7%)、CD13(83.7%)、CD33(83.7%)，단균불표체CD34、HLA-DR，차SSC교대；M4、M5중가견CD64、CD4、CD14명현표체고우AML기타아형(P<0.01)。19례B-ALL환자중CD19적표체솔최고(100%)，기차위CD34(90.1%)、HLA-DR (90.1%)、CD10(90.1%)；9례T-ALL환자중CD7적표체솔최고(100%)，기차위CD4(77.8%)、CD5(77.8%)、CD2(66.7%)、CD3(66.7%)、CD34(55.6%)。결론통과오색류식세포술진행백혈병면역분형시형태학분형진단중요적보충，능구제공경객관적진단의거，대제고급성백혈병적진단정학솔、예후판단화치료방안적선택유중요적의의。
Objective To study the relationship between the immunophenotype by five-color flow cytometry and FAB phenotype in Acute leukemia. Methods Immunophenotyping of 118 cases of bone marrow specimens from patients with acute leukemia were analyzed by five-color FCM with CD45/SSC double parameters and scatter spot picture. Results In 88 cases of acute myeloid leukemia (AML), the myeloid-associated antigens were CD33 (93.2%), CD13 (88.6%), and CD117 (11.3%), respectively, and the lymphoid-associated antigens were CD56 (25.2%), CD7 (11.3%), CD19 (2.2%) and CD20 (2.2%), respectively. The antigens mainly expressed in M3 were CD117 (83.7%), CD13 (83.7%), CD33 (83.7%), and bigger SSC, but not CD34 and HLA-DR. The antigens mainly expressed in M4 and M5 were CD64, CD4 and CD14 (P<0.01). In 19 cases of patients with B-ALL, the expression rate of CD19 was the highest (100%), followed by CD34 (90.1%), HLA-DR (90.1%) and CD10 (90.1%). In 9 cases of patients with T-ALL, the ex-pression rate of CD7 was the highest (100%), followed by CD4 (77.8%), CD5 (77.8%), CD2 (66.7%), CD3 (66.7%) and CD34 (55.6%). Conclusion Based on a more objective diagnostic basis, five-color flow cytometry is extremely impor-tant in improving the diagnostic accuracy, therapy and prognosis in acute leukemia.