CAJ | 학술논문

目的：观察电针“四关”组穴对脑卒中后抑郁（post-stroke depression，PSD）大鼠海马组织中单胺类神经递质五羟色胺（5-HT）、去甲肾上腺素（NE）和多巴胺（DA）水平的影响并探讨其作用机制。方法按随机数字表法将大鼠随机分为对照组、大脑中动脉闭塞（MCAO）组、PSD 组、电针组、氟西汀组，各10只。除对照组外，其余大鼠采用线栓法制备脑缺血模型，除MCAO组外，其余大鼠于MCAO造模成功7 d后予以孤养加用慢性不可预见性温应激（CUMS）方法建立PSD模型。其中电针组予以电针“四关”组穴治疗，氟西汀组灌胃氟西汀溶液2.33 mg/(kg?d)，对照组、MCAO组、PSD组灌胃等体积蒸馏水，1次/d，连续干预21 d。于干预第1、7、14、21天进行糖水实验，干预第22天，处死大鼠取脑，采用RP-HPLC-荧光检测法测定大鼠海马组织5-HT、NE、DA水平。结果与PSD组比较，电针组大鼠糖水消耗量治疗第7天[（51.09±3.68）%比（38.97±4.07）%]、第14天[（68.25±4.69）%比（39.64±4.49）%]、第21天[（41.56±2.12）%比（41.56±2.12）%]明显升高（P＜0.01）；治疗后21 d，电针组大鼠海马组织中5-HT[（526.31±54.41）ng/g比（221.56±39.57）ng/g]、NE[（244.93±44.57）ng/g比（129.27±11.68）ng/g]、DA[（86.80±14.64）ng/g比（44.22±7.60）ng/g]水平较PSD 组显著升高（P＜0.01）。结论电针“四关”组穴可改善PSD大鼠快感消失症状，其作用机制可能与增加大鼠海马组织中单胺类神经递质水平相关。
목적：관찰전침“사관”조혈대뇌졸중후억욱（post-stroke depression，PSD）대서해마조직중단알류신경체질오간색알（5-HT）、거갑신상선소（NE）화다파알（DA）수평적영향병탐토기작용궤제。방법안수궤수자표법장대서수궤분위대조조、대뇌중동맥폐새（MCAO）조、PSD 조、전침조、불서정조，각10지。제대조조외，기여대서채용선전법제비뇌결혈모형，제MCAO조외，기여대서우MCAO조모성공7 d후여이고양가용만성불가예견성온응격（CUMS）방법건립PSD모형。기중전침조여이전침“사관”조혈치료，불서정조관위불서정용액2.33 mg/(kg?d)，대조조、MCAO조、PSD조관위등체적증류수，1차/d，련속간예21 d。우간예제1、7、14、21천진행당수실험，간예제22천，처사대서취뇌，채용RP-HPLC-형광검측법측정대서해마조직5-HT、NE、DA수평。결과여PSD조비교，전침조대서당수소모량치료제7천[（51.09±3.68）%비（38.97±4.07）%]、제14천[（68.25±4.69）%비（39.64±4.49）%]、제21천[（41.56±2.12）%비（41.56±2.12）%]명현승고（P＜0.01）；치료후21 d，전침조대서해마조직중5-HT[（526.31±54.41）ng/g비（221.56±39.57）ng/g]、NE[（244.93±44.57）ng/g비（129.27±11.68）ng/g]、DA[（86.80±14.64）ng/g비（44.22±7.60）ng/g]수평교PSD 조현저승고（P＜0.01）。결론전침“사관”조혈가개선PSD대서쾌감소실증상，기작용궤제가능여증가대서해마조직중단알류신경체질수평상관。
Objective To observe the effect of electroacupuncture of "Siguan" on contents of hippocampal monoamine neurotransmitter NE, DA and 5-HT in post-stroke depression rats, and throught it to probe the possible mechanism of anti-depressive.Methods 60 rats were divided into five groups randomly, as the normal group, MCAO group, PSD group, Siguan group, and fluoxetine group. Except the normal group, the rats of other groups were used to establish left side of the MCAO models by modified Longa filament method. PSD model was prepared by living alone in combination with mild stimulation by chronic unpredictable after stroke model success 7 days. The Siguan group was received electroacupuncture at "Siguan" point, and the fluoxetine group was gastrically perfused with fluoxetine, and all rats were received sugar consumption experiment in different period of treatment. After continuous treatment for 21 days, contents of NE, DA and 5-HT in hippocampal tissue were measured with RP-HPLC-fluorescent method. Results At the seventh day, compared with PSD group, the ratio of sugar water consumption(51.09%± 3.68%vs. 38.97%± 4.07%)has a significant increase in the Siguan group(P<0.01), while no significant changes in the fluoxetine group. At the fourteenth day, the ratio of sugar water consumption(68.25%± 4.69%vs. 39.64%± 4.49%)continued to rise in the Siguan group, the fluoxetine group also had significantly increased compared with the PSD group, the two groups were significantly higher than those in the PSD group (P<0.01), but the Siguan group was higher than that of the fluoxetine group(P<0.01).At the twenty-first day, the ratio of sugar water consumption (41.56%± 2.12%vs. 41.56%± 2.12%)increased further in the Siguan group and the fluoxetine group, which was significantly higher than those in the PSD group (P<0.01), but the Siguan group and fluoxetine group had no obvious difference(P>0.05). Compared with the PSD group,the contents of NE(244.93 ± 44.57 ng/g vs. 129.27 ± 11.68 ng/g), DA(86.80 ± 14.64 ng/g vs.44.22 ± 7.60 ng/g), 5-HT(526.31 ± 54.41 ng/g vs.221.56 ± 39.57 ng/g) in the hippocampus tissues were significantly higher than many in the Siguan group and fluoxetine group(P<0.01), but the difference between the two groups was not obvious(P>0.05). Conclusion The symptoms of PSD rats pleasure disappeared can be improved by electroacupuncture at"Siguan". Its mechanism may be related with the increased contents of monoamine neurotransmitters in the hippocampus of rats.