CAJ | 학술논문

目的：探讨欣怡胶囊预处理对兔心肌缺血再灌注损伤的保护作用及其机制。方法94只兔按体质量随机分为模型组，替罗非班组，欣怡高、中、低剂量组（4.0、2.0、1.0 g/kg），每组16只，假手术组14只。连续欣怡胶囊灌胃5 d 后，结扎冠状动脉左回旋支建立AMI再灌注模型。记录模型制作前后心电图。检测血清髓过氧化物酶（MPO）、乳酸脱氢酶（LDH）和肌酸激酶-MB（CK-MB）水平。评价心肌组织病理学。结果欣怡高、中、低剂量组兔心电图中J点振幅变化值[分别为(0.064±0.049)mV、(0.069±0.061)mV、(0.079±0.060)mV]较模型组(0.158±0.105)mV显著降低（P＜0.01或P＜0.05）；欣怡高、中、低剂量组兔血清 LDH[分别为(399.7±202.3)U/L、(369.6±229.0)U/L、(435.5±152.4)U/L]、CK-MB[分别为(900.8±231.2)U/L、(1268.3±899.8)U/L、(1386.7±621.6)U/L]、MPO[分别为(69.81±5.51)U/L、(85.44±10.31)U/L、(81.33±16.87)U/L]浓度较模型组[LDH(817.1±401.9)U/L、CK-MB(2071.3±693.5)U/L、MPO(149.9±20.11)U/L]显著下降（P＜0.01或P＜0.05）；HE染色结果显示，欣怡高、中、低剂量组心肌损伤较模型组显著减轻。结论欣怡胶囊预处理可保护兔心肌缺血再灌注损伤，其机制可能与抑制炎症反应有关。
목적：탐토흔이효낭예처리대토심기결혈재관주손상적보호작용급기궤제。방법94지토안체질량수궤분위모형조，체라비반조，흔이고、중、저제량조（4.0、2.0、1.0 g/kg），매조16지，가수술조14지。련속흔이효낭관위5 d 후，결찰관상동맥좌회선지건립AMI재관주모형。기록모형제작전후심전도。검측혈청수과양화물매（MPO）、유산탈경매（LDH）화기산격매-MB（CK-MB）수평。평개심기조직병이학。결과흔이고、중、저제량조토심전도중J점진폭변화치[분별위(0.064±0.049)mV、(0.069±0.061)mV、(0.079±0.060)mV]교모형조(0.158±0.105)mV현저강저（P＜0.01혹P＜0.05）；흔이고、중、저제량조토혈청 LDH[분별위(399.7±202.3)U/L、(369.6±229.0)U/L、(435.5±152.4)U/L]、CK-MB[분별위(900.8±231.2)U/L、(1268.3±899.8)U/L、(1386.7±621.6)U/L]、MPO[분별위(69.81±5.51)U/L、(85.44±10.31)U/L、(81.33±16.87)U/L]농도교모형조[LDH(817.1±401.9)U/L、CK-MB(2071.3±693.5)U/L、MPO(149.9±20.11)U/L]현저하강（P＜0.01혹P＜0.05）；HE염색결과현시，흔이고、중、저제량조심기손상교모형조현저감경。결론흔이효낭예처리가보호토심기결혈재관주손상，기궤제가능여억제염증반응유관。
Objective To investigate the protective effect of Xinyi capsule pretreatment on myocardial ischemia reperfusion injury in rabbits and its possible mechanism. Methods Ninety-four rabbits were randomly divided into 6 groups: model group (n=16), tirofiban group (n=16), high-, medium- and low-dose Xinyi capsule groups (4.0, 2.0, 1.0 g/kg;n=16 in each group), and sham operation group (n=14). Five days after intragastric administration with drug, myocardial ischemia reperfusion was induced by ligation of the proximal left circumflex artery. The electrocardiogram (ECG) was continuously recorded. The serum levels of myeloperoxidase (MPO), lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) were measured. Myocardial histopathological damage was evaluated. Results The changes of J-point amplitude on ECG in high-, medium-and low-dose Xinyi capsule groups (0.064 ± 0.049 mV, 0.069 ± 0.061 mV, 0.079 ± 0.060 mV) were significantly lower than that in the model group (0.158 ± 0.105 mV, P<0.01 or P<0.05), the serum levels of LDH (399.7 ± 202.3 U/L, 369.6 ± 229.0 U/L, 435.5 ± 152.4 U/L), CK-MB (900.8 ± 231.2 U/L, 1 268.3 ± 899.8 U/L, 1 386.7 ± 621.6 U/L), MPO (69.81 ± 5.51 U/L, 85.44 ± 10.31 U/L, 81.33 ± 16.87 U/L) were significantly lower than those in the model group (LDH:817.1 ± 401.9 U/L, CK-MB:2 071.3 ± 693.5 U/L, MPO:149.9 ± 20.11 U/L;P<0.01 or P<0.05). Histopathological examination showed that myocardial damage in high-, medium- and low-dose Xinyi capsule groups reduced compared with the model group. Conclusions Xinyi capsule pretreatment can protect against myocardial ischemia reperfusion injury in rabbits, and its mechanism may be related to inflammation inhibition.