国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2015年
1期
52-55
,共4页
非酒精性脂肪性肝病%肝硬化,实验性%绞股蓝%生物学标记%C-反应蛋白%兔
非酒精性脂肪性肝病%肝硬化,實驗性%絞股藍%生物學標記%C-反應蛋白%兔
비주정성지방성간병%간경화,실험성%교고람%생물학표기%C-반응단백%토
Non-alcoholic fatty liver disease%Liver cirrhosis,experimental%Gynostemma Pentaphyllum%Biological markers%C-Reactive protein%Rabbits
目的：探讨绞股蓝对非酒精性脂肪性肝病（nonalcoholic fatty liver disease, NAFLD）兔肝纤维化血清标记物的影响。方法将40只成年雄性兔按随机数字表法随机分为绞股蓝组、辛伐他汀组、模型组、正常对照组，每组10只。正常对照组给予标准饲料喂养，其余动物给予高脂饲料100 g/d喂养，建立NAFLD模型。绞股蓝组灌胃绞股蓝水煎液5 g/(kg?d)，辛伐他汀组灌胃辛伐他汀溶液5 mg/(kg?d)，空白组及模型组灌胃等体积蒸馏水。连续给药9周。检测血清总胆固醇(TC)、三酰甘油(TG)、C-反应蛋白(CRP)、透明质酸(HA)、层黏连蛋白(LN)水平及肝组织TG水平。结果与正常对照组比较，模型组血清TG[(1.72±0.61)mmol/L比(0.85±0.39)mmol/L]、TC[(60.50±9.77)mg/L比(1.30±0.44)mg/L]、CRP[(256.79±30.78)mg/L比(8.71±1.41)mg/L]、HA[(798.8±69.4)ng/ml比(121.2±6.8)ng/ml]、LN[(964.8±62.8)ng/ml 比(142.4±12.2)ng/ml]显著升高（P＜0.01）。与模型组比较，绞股蓝组血清TG[(1.31±0.39)mmol/L 比(1.72±0.61)mmol/L]、TC[(36.44±6.57)mmol/L比(60.50±9.77)mmol/L]、CRP[(68.77±10.78)mg/L比(256.79±30.78)mg/L]、HA[(420.8±60.2)ng/ml比(798.8±69.4)ng/ml]、LN[(449.8±56.6)ng/ml比(964.8±62.8)ng/ml]以及肝组织TG[(0.52±0.10)mmol/L比(0.77±0.08)mmol/L]显著降低（P＜0.01）。绞股蓝组肝组织TG水平[(0.52±0.10)mmol/L比(0.59±0.09)mmol/L]较辛伐他汀组显著降低（P＜0.05），但血清TC、TG、CRP、HA和LN水平与辛伐他汀组比较，差异无统计学意义（P＞0.05）。结论绞股蓝可调节NAFLD兔血脂代谢，减轻炎症反应，降低肝纤维化血清血清标记物水平，可能对肝纤维化有保护作用。
目的：探討絞股藍對非酒精性脂肪性肝病（nonalcoholic fatty liver disease, NAFLD）兔肝纖維化血清標記物的影響。方法將40隻成年雄性兔按隨機數字錶法隨機分為絞股藍組、辛伐他汀組、模型組、正常對照組，每組10隻。正常對照組給予標準飼料餵養，其餘動物給予高脂飼料100 g/d餵養，建立NAFLD模型。絞股藍組灌胃絞股藍水煎液5 g/(kg?d)，辛伐他汀組灌胃辛伐他汀溶液5 mg/(kg?d)，空白組及模型組灌胃等體積蒸餾水。連續給藥9週。檢測血清總膽固醇(TC)、三酰甘油(TG)、C-反應蛋白(CRP)、透明質痠(HA)、層黏連蛋白(LN)水平及肝組織TG水平。結果與正常對照組比較，模型組血清TG[(1.72±0.61)mmol/L比(0.85±0.39)mmol/L]、TC[(60.50±9.77)mg/L比(1.30±0.44)mg/L]、CRP[(256.79±30.78)mg/L比(8.71±1.41)mg/L]、HA[(798.8±69.4)ng/ml比(121.2±6.8)ng/ml]、LN[(964.8±62.8)ng/ml 比(142.4±12.2)ng/ml]顯著升高（P＜0.01）。與模型組比較，絞股藍組血清TG[(1.31±0.39)mmol/L 比(1.72±0.61)mmol/L]、TC[(36.44±6.57)mmol/L比(60.50±9.77)mmol/L]、CRP[(68.77±10.78)mg/L比(256.79±30.78)mg/L]、HA[(420.8±60.2)ng/ml比(798.8±69.4)ng/ml]、LN[(449.8±56.6)ng/ml比(964.8±62.8)ng/ml]以及肝組織TG[(0.52±0.10)mmol/L比(0.77±0.08)mmol/L]顯著降低（P＜0.01）。絞股藍組肝組織TG水平[(0.52±0.10)mmol/L比(0.59±0.09)mmol/L]較辛伐他汀組顯著降低（P＜0.05），但血清TC、TG、CRP、HA和LN水平與辛伐他汀組比較，差異無統計學意義（P＞0.05）。結論絞股藍可調節NAFLD兔血脂代謝，減輕炎癥反應，降低肝纖維化血清血清標記物水平，可能對肝纖維化有保護作用。
목적：탐토교고람대비주정성지방성간병（nonalcoholic fatty liver disease, NAFLD）토간섬유화혈청표기물적영향。방법장40지성년웅성토안수궤수자표법수궤분위교고람조、신벌타정조、모형조、정상대조조，매조10지。정상대조조급여표준사료위양，기여동물급여고지사료100 g/d위양，건립NAFLD모형。교고람조관위교고람수전액5 g/(kg?d)，신벌타정조관위신벌타정용액5 mg/(kg?d)，공백조급모형조관위등체적증류수。련속급약9주。검측혈청총담고순(TC)、삼선감유(TG)、C-반응단백(CRP)、투명질산(HA)、층점련단백(LN)수평급간조직TG수평。결과여정상대조조비교，모형조혈청TG[(1.72±0.61)mmol/L비(0.85±0.39)mmol/L]、TC[(60.50±9.77)mg/L비(1.30±0.44)mg/L]、CRP[(256.79±30.78)mg/L비(8.71±1.41)mg/L]、HA[(798.8±69.4)ng/ml비(121.2±6.8)ng/ml]、LN[(964.8±62.8)ng/ml 비(142.4±12.2)ng/ml]현저승고（P＜0.01）。여모형조비교，교고람조혈청TG[(1.31±0.39)mmol/L 비(1.72±0.61)mmol/L]、TC[(36.44±6.57)mmol/L비(60.50±9.77)mmol/L]、CRP[(68.77±10.78)mg/L비(256.79±30.78)mg/L]、HA[(420.8±60.2)ng/ml비(798.8±69.4)ng/ml]、LN[(449.8±56.6)ng/ml비(964.8±62.8)ng/ml]이급간조직TG[(0.52±0.10)mmol/L비(0.77±0.08)mmol/L]현저강저（P＜0.01）。교고람조간조직TG수평[(0.52±0.10)mmol/L비(0.59±0.09)mmol/L]교신벌타정조현저강저（P＜0.05），단혈청TC、TG、CRP、HA화LN수평여신벌타정조비교，차이무통계학의의（P＞0.05）。결론교고람가조절NAFLD토혈지대사，감경염증반응，강저간섬유화혈청혈청표기물수평，가능대간섬유화유보호작용。
Objective To investigate the effects of Gynostemma Pentaphyllum on serum markers of liver fibrosis in cholesterol-induced non-alcoholic fatty liver disease (NAFLD) in rabbits. Methods Forty adult male white rabbits were randomly divided into a normal control group, a model group, a treatment group and a simvastatin group, with 10 rabbits in each group. NAFLD was induced with a high-cholesterol diet. After modeling, the rabbits in the treatment group were intragastrically administrated with Gynostemma Pentaphyllum 5 mg/(kg?d), the simvastatin group with simvastatin 5 mg/(kg?d), and the model and normal control groups with the equal volume of distilled water for 9 weeks. The serum levels of total cholesterol (TC) and triglyceride (TG), the serum inflammatory maker C-reactive protein (CRP), the serum markers of liver fibrosis such as hyaluronic acid and laminin (LN), and the TG level in the liver tissue were detected. Results Compared with the normal control group, the serum levels of TC (60.50 ± 9.77 mg/L vs.1.30 ± 0.44 mg/L), TG (1.72 ± 0.61 mmol/L vs. 0.85 ± 0.39 mmol/L), CRP (256.79 ± 30.78 mg/L vs. 8.71 ± 1.41 mg/L), HA (798.8 ± 69.4 ng/ml vs. 121.2 ± 6.8 ng/ml),LN (964.8 ± 62.8 ng/ml vs. 142.4 ± 12.2 ng/ml) in the model group were increased significantly (all P<0.01). Compared with the model group, the serum levels of TC (36.44 ± 6.57 mmol/L vs. 60.50 ± 9.77 mmol/L), TG (1.31 ± 0.39 mmol/L vs. 1.72 ± 0.61 mmol/L), CRP (68.77 ± 10.78 mg/L vs. 256.79 ± 30.78 mg/L), HA (420.8 ± 60.2 ng/ml vs. 798.8 ± 69.4 ng/ml), LN (449.8 ± 56.6 ng/ml vs. 964.8 ± 62.8 ng/ml) and the TG level in the liver tissue (0.52 ± 0.10 mmol/L vs. 0.77 ± 0.08 mmol/L) in the treatment group were decreased significantly (all P<0.01). The TG level in the liver tissue in the treatment group was significantly lower than that in the simvastatin group (0.52 ± 0.10 mmol/L vs. 0.59 ± 0.09 mmol/L;P<0.05). There were no significant difference in the serum levels of TC, TG,CRP,HA and LN between the treatment group and the simvastatin group (all P>0.05). Conclusions Gynostemma Pentaphyllum may regulate lipid metabolism, alleviate inflammation, and decrease serum markers of liver fibrosis, and might protect against liver fibrosis in rabbits with NAFLD.
