国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2015年
1期
47-51
,共5页
那坤%武亮%李滢%郑莹%谢晶日
那坤%武亮%李瀅%鄭瑩%謝晶日
나곤%무량%리형%정형%사정일
肝硬化,实验性%中草药%信号转导%转化生长因子-β1%Smad蛋白%大鼠%软肝颗粒
肝硬化,實驗性%中草藥%信號轉導%轉化生長因子-β1%Smad蛋白%大鼠%軟肝顆粒
간경화,실험성%중초약%신호전도%전화생장인자-β1%Smad단백%대서%연간과립
Liver cirrhosis,experimental%Drugs,Chinese herbal%Signal transduction%Transforming growth factorβ1%Smad proteins%Rats%Ruangan granule
目的：探讨软肝颗粒对肝纤维化大鼠转化生长因子-β1(TGF-β1)/Smads 信号通路的影响。方法105只Wistar大鼠，按随机数字表法随机分为正常对照组，模型组，秋水仙碱组，大黄?虫丸组，软肝颗粒高、中、低剂量组，每组15只。采用四氯化碳和高胆固醇饮食诱导肝纤维化。模型制作后，软肝颗粒低、中、高剂量组分别灌胃软肝颗粒混悬液3.600、7.200、14.400 g/(kg?d)；大黄?虫丸组灌胃大黄?虫丸混悬液0.180 g/(kg?d)；秋水仙碱组灌胃秋水仙碱混悬液0.108 mg/(kg?d)；正常对照组和模型组以等体积蒸馏水灌胃。均连续灌胃8周。采用免疫组化染色法检测肝组织TGF-β1、Smad3、Smad7表达，运用 RT-PCR 技术检测肝组织 TGF-β1、Smad3、Smad7 mRNA 表达。结果与正常对照组比较，模型组TGF-β1[(2.59±0.99)比(0.43±0.21)]、Smad3[(2.56±0.67)比(0.41±0.18)]蛋白及 TGF-β1 mRNA[(2.25±0.21)比(0.71±0.09)]、Smad3 mRNA[(2.34±0.03)比(0.78±0.12)]表达显著升高（P均＜0.01）。与模型组比较，软肝颗粒高剂量组TGF-β1[(1.12±0.27)比(2.59±0.99)]、Smad3蛋白[(1.05±0.34)比(2.56±0.67)]表达下降，Smad7蛋白表达[(2.33±0.62)比(0.36±0.18)]升高（P＜0.01），TGF-β1 mRNA[(1.09±0.11)比(2.25±0.21)]、Smad3 mRNA[(1.10±0.02)比(2.34±0.03)]表达下降，Smad7 mRNA[(1.18±0.13)比(0.38±0.11)]表达升高（P＜0.05）。结论软肝颗粒可通过下调TGF-β1、Smad3，上调Smad7调节肝纤维化大鼠TGF-β1/Smads信号通路。
目的：探討軟肝顆粒對肝纖維化大鼠轉化生長因子-β1(TGF-β1)/Smads 信號通路的影響。方法105隻Wistar大鼠，按隨機數字錶法隨機分為正常對照組，模型組，鞦水仙堿組，大黃?蟲汍組，軟肝顆粒高、中、低劑量組，每組15隻。採用四氯化碳和高膽固醇飲食誘導肝纖維化。模型製作後，軟肝顆粒低、中、高劑量組分彆灌胃軟肝顆粒混懸液3.600、7.200、14.400 g/(kg?d)；大黃?蟲汍組灌胃大黃?蟲汍混懸液0.180 g/(kg?d)；鞦水仙堿組灌胃鞦水仙堿混懸液0.108 mg/(kg?d)；正常對照組和模型組以等體積蒸餾水灌胃。均連續灌胃8週。採用免疫組化染色法檢測肝組織TGF-β1、Smad3、Smad7錶達，運用 RT-PCR 技術檢測肝組織 TGF-β1、Smad3、Smad7 mRNA 錶達。結果與正常對照組比較，模型組TGF-β1[(2.59±0.99)比(0.43±0.21)]、Smad3[(2.56±0.67)比(0.41±0.18)]蛋白及 TGF-β1 mRNA[(2.25±0.21)比(0.71±0.09)]、Smad3 mRNA[(2.34±0.03)比(0.78±0.12)]錶達顯著升高（P均＜0.01）。與模型組比較，軟肝顆粒高劑量組TGF-β1[(1.12±0.27)比(2.59±0.99)]、Smad3蛋白[(1.05±0.34)比(2.56±0.67)]錶達下降，Smad7蛋白錶達[(2.33±0.62)比(0.36±0.18)]升高（P＜0.01），TGF-β1 mRNA[(1.09±0.11)比(2.25±0.21)]、Smad3 mRNA[(1.10±0.02)比(2.34±0.03)]錶達下降，Smad7 mRNA[(1.18±0.13)比(0.38±0.11)]錶達升高（P＜0.05）。結論軟肝顆粒可通過下調TGF-β1、Smad3，上調Smad7調節肝纖維化大鼠TGF-β1/Smads信號通路。
목적：탐토연간과립대간섬유화대서전화생장인자-β1(TGF-β1)/Smads 신호통로적영향。방법105지Wistar대서，안수궤수자표법수궤분위정상대조조，모형조，추수선감조，대황?충환조，연간과립고、중、저제량조，매조15지。채용사록화탄화고담고순음식유도간섬유화。모형제작후，연간과립저、중、고제량조분별관위연간과립혼현액3.600、7.200、14.400 g/(kg?d)；대황?충환조관위대황?충환혼현액0.180 g/(kg?d)；추수선감조관위추수선감혼현액0.108 mg/(kg?d)；정상대조조화모형조이등체적증류수관위。균련속관위8주。채용면역조화염색법검측간조직TGF-β1、Smad3、Smad7표체，운용 RT-PCR 기술검측간조직 TGF-β1、Smad3、Smad7 mRNA 표체。결과여정상대조조비교，모형조TGF-β1[(2.59±0.99)비(0.43±0.21)]、Smad3[(2.56±0.67)비(0.41±0.18)]단백급 TGF-β1 mRNA[(2.25±0.21)비(0.71±0.09)]、Smad3 mRNA[(2.34±0.03)비(0.78±0.12)]표체현저승고（P균＜0.01）。여모형조비교，연간과립고제량조TGF-β1[(1.12±0.27)비(2.59±0.99)]、Smad3단백[(1.05±0.34)비(2.56±0.67)]표체하강，Smad7단백표체[(2.33±0.62)비(0.36±0.18)]승고（P＜0.01），TGF-β1 mRNA[(1.09±0.11)비(2.25±0.21)]、Smad3 mRNA[(1.10±0.02)비(2.34±0.03)]표체하강，Smad7 mRNA[(1.18±0.13)비(0.38±0.11)]표체승고（P＜0.05）。결론연간과립가통과하조TGF-β1、Smad3，상조Smad7조절간섬유화대서TGF-β1/Smads신호통로。
Objective To investigate the effects of Ruangan granule on transforming growth factor-β1(TGF-β1)/Smads signaling pathway in liver fibrosis in rats. Methods A total of 105 Wistar rats were randomly divided into normal control group, model group and colchicine, Dahuang-Zhechong pill group, high-, medium- and low-dose Ruangan granule groups (n=15 in each group). Liver fibrosis was induced by carbon tetrachloride and a high-cholesterol diet. After modeling, the low-, medium- and high-dose Ruangan granule groups were intragastric administrated Ruangan granule mixed suspension 3.6, 7.2, 14.4 g/(kg?d), respectively;Dahuang-Zhechong pill group was administrated with Dahuang-Zhechong pellets mixed suspension of 0.18 g/(kg?d);the colchicine group was intragastric administrated with colchicine mixed suspension of 0.108 mg/(kg?d);and the normal control group and the model group were intragastric administrated with the equal volume of distilled water. All rats were intragastric administrated for 8 weeks. The expressions of TGF-β1, Smad3 and Smad7 proteins in the liver tissue were detected with immunohistochemical staining method. The expressions of TGF-β1, Smad3, Smad7 mRNAs in the liver tussue were detected by RT-PCR. Results The expressions of TGF-β1 (2.59 ± 0.99 vs. 0.43 ± 0.21) and Smad3 (2.56 ± 0.67 vs. 0.41 ± 0.18) proteins and TGF-β1 mRNA (2.25 ± 0.21 vs. 0.71 ± 0.09) and Smad3 (2.34 ± 0.03 vs. 0.78 ± 0.12) mRNAs in the model group were significantly increased than those in the normal control group (all P<0.01). Compared with the model group, the expressions of TGF-β1 (1.12 ± 0.27 vs. 2.59 ± 0.99) and Smad3 (1.05 ± 0.34 vs. 2.56 ± 0.67) proteins in the high-dose Ruangan granule group decreased significantly, the expression of Smad7 increased significantly (2.33 ± 0.62 vs. 0.36 ± 0.18), and the expressions of TGF-β1 (1.09 ± 0.11 vs. 2.25 ± 0.21) and Smad3 (1.10 ± 0.02 vs. 2.34 ± 0.03) mRNAs decreased significantly, the expression of smad7 mRNA (1.18 ± 0.13 vs. 0.38 ± 0.11) increased significantly (P<0.05). Conclusions Ruangan granule can regulate the TGF-β1/Smads signaling pathway via down-regulation of TGF-β1, Smad3 and up-regulation of Smad7 in liver fibrosis in rats.
