CAJ | 학술논문

目的：：建立以高效液相色谱法测定银杏叶缓释微丸胶囊中山奈素、异鼠李素、槲皮素含量的方法。方法：以C 18为色谱柱，流动相为甲醇∶0.4%磷酸溶液（45∶55），检测波长为360nm，流速为1ml/min，柱温为30℃，进样量是10μl。结果：槲皮素在0.1505～0.9030μg（r＝0.9999）浓度范围内有良好的线性关系，平均回收率为99.86％，RSD=0.4％；山奈素在0.1450～0.8940μg（r＝0.9998）浓度范围内有良好的线性关系，平均回收率为99.79％，RSD=0.5％；异鼠李素在0.0995～0.5970μg（r＝0.9998）浓度范围内有良好的线性关系，平均回收率为99.80％，RSD=0.3％。结论：本方法简便、准确、专属性强，可用于该制剂的质量控制。
목적：：건립이고효액상색보법측정은행협완석미환효낭중산내소、이서리소、곡피소함량적방법。방법：이C 18위색보주，류동상위갑순∶0.4%린산용액（45∶55），검측파장위360nm，류속위1ml/min，주온위30℃，진양량시10μl。결과：곡피소재0.1505～0.9030μg（r＝0.9999）농도범위내유량호적선성관계，평균회수솔위99.86％，RSD=0.4％；산내소재0.1450～0.8940μg（r＝0.9998）농도범위내유량호적선성관계，평균회수솔위99.79％，RSD=0.5％；이서리소재0.0995～0.5970μg（r＝0.9998）농도범위내유량호적선성관계，평균회수솔위99.80％，RSD=0.3％。결론：본방법간편、준학、전속성강，가용우해제제적질량공제。
Objective: To establish a method for the determination of haempferol, isorhamnetin and quercetin in folium ginkgo pellets capsules Methods: HPLC was performed on a C18 column using mathanol∶0.4%H3PO4 (45∶55)as the mobile phase at a flow rate of 1ml/min. The detection wavelength was at 360nm. The column temperature was 30℃and the sample size was 10μl. Results:The linear range of quercetin was 0.1505~0.9030μg (r=0.9999), the average recovery was 99.86%(RSD=0.4%); The linear range of haempferol was 0.1450~0.8940μg (r=0.9998), the average recovery was 99.79%(RSD=0.5%);The linear range of isorhamnetin was 0.0995~0.5970μg (r=0.9998), the average recovery was 99.80%(RSD=0.3%);Conclusions:The method is simple, accurate, special and can be used for the quality control of folium ginkgo pellets capsules.