中国社区医师
中國社區醫師
중국사구의사
Chinese Community Doctors
2015年
2期
113-114
,共2页
抗中性粒细胞胞浆抗体%间接免疫荧光法%肾脏病
抗中性粒細胞胞漿抗體%間接免疫熒光法%腎髒病
항중성립세포포장항체%간접면역형광법%신장병
Anti neutrophil cytoplasmic antibody%Indirect immunofluorescence method%Kidney disease
目的：探讨抗中性粒细胞胞浆抗体(ANCA)的检测在肾脏病诊断中的临床意义。方法：应用间接免疫荧光(ⅡF)法检测抗ANCA，联合ELISA方法检测过氧化物酶(MPO)和蛋白酶3(PR3)，观察99例肾脏病患者血清抗中性粒细胞胞浆抗体的阳性率。结果：应用ⅡF检测ANCA，联合ELISA检测MPO和PR3，肾脏病ANCA阳性率37%，其中肾小球肾炎、紫癜性肾炎、狼疮性肾炎阳性率分别为57%、21%和20%。结论：ANCA在肾小球肾炎和紫癜性肾炎中阳性率高，有重要诊断意义。
目的：探討抗中性粒細胞胞漿抗體(ANCA)的檢測在腎髒病診斷中的臨床意義。方法：應用間接免疫熒光(ⅡF)法檢測抗ANCA，聯閤ELISA方法檢測過氧化物酶(MPO)和蛋白酶3(PR3)，觀察99例腎髒病患者血清抗中性粒細胞胞漿抗體的暘性率。結果：應用ⅡF檢測ANCA，聯閤ELISA檢測MPO和PR3，腎髒病ANCA暘性率37%，其中腎小毬腎炎、紫癜性腎炎、狼瘡性腎炎暘性率分彆為57%、21%和20%。結論：ANCA在腎小毬腎炎和紫癜性腎炎中暘性率高，有重要診斷意義。
목적：탐토항중성립세포포장항체(ANCA)적검측재신장병진단중적림상의의。방법：응용간접면역형광(ⅡF)법검측항ANCA，연합ELISA방법검측과양화물매(MPO)화단백매3(PR3)，관찰99례신장병환자혈청항중성립세포포장항체적양성솔。결과：응용ⅡF검측ANCA，연합ELISA검측MPO화PR3，신장병ANCA양성솔37%，기중신소구신염、자전성신염、랑창성신염양성솔분별위57%、21%화20%。결론：ANCA재신소구신염화자전성신염중양성솔고，유중요진단의의。
Objective:To investigate the clinical significance of anti neutrophil cytoplasmic antibody(ANCA) detection in renal diseases.Methods:We used the indirect immunofluorescence(ⅡF) assay to detect the anti neutrophil cytoplasmic antibodies,and combined with ELISA method for the detection of myeloperoxidase(MPO) and proteinase 3(PR3),and we observed the positive rate of serum anti neutrophil cytoplasmic antibody in 99 patients with kidney disease.Results:We used the ⅡF to detect the ANCA, and combined the ELISA to detect MPO and PR3.The positive rate of ANCA in kidney disease was 37%,and glomerulonephritis, purpura nephritis,lupus nephritis positive rates were 57%,21% and 20% respectively.Conclusion:The positive rate of ANCA in glomerular nephritis and purpuric nephritis is high,and there is important significance in diagnosis.
