CAJ | 학술논문

目的：探讨长链非编码RNA Gas5（lncRNA growth arrest-specific 5，lncRNA Gas5）在非小细胞肺癌（NSCLC）中的临床意义。方法收集2009年2月至2011年2月我院50例NSCLC手术切除标本，qRT-PCR检测肿瘤和正常肺组织中Gas5的表达，同时比较肺癌细胞株和正常支气管上皮细胞株中Gas5的表达差异，并分析Gas5与临床病理及预后的关系。结果肺癌组织中的Gas5的表达水平显著低于正常肺组织中的表达水平（P＜0.001）。肺癌组织中Gas5的表达水平与肿瘤的大小、淋巴结转移及临床分期明显相关（P＜0.05）。Gas5低表达者生存时间明显低于高表达者，差异有显著统计学意义（P＜0.05）。结论 LncRNA Gas5在NSCLC组织中表达下调，可能成为NSCLC判断预后的新的分子标记物。
목적：탐토장련비편마RNA Gas5（lncRNA growth arrest-specific 5，lncRNA Gas5）재비소세포폐암（NSCLC）중적림상의의。방법수집2009년2월지2011년2월아원50례NSCLC수술절제표본，qRT-PCR검측종류화정상폐조직중Gas5적표체，동시비교폐암세포주화정상지기관상피세포주중Gas5적표체차이，병분석Gas5여림상병리급예후적관계。결과폐암조직중적Gas5적표체수평현저저우정상폐조직중적표체수평（P＜0.001）。폐암조직중Gas5적표체수평여종류적대소、림파결전이급림상분기명현상관（P＜0.05）。Gas5저표체자생존시간명현저우고표체자，차이유현저통계학의의（P＜0.05）。결론 LncRNA Gas5재NSCLC조직중표체하조，가능성위NSCLC판단예후적신적분자표기물。
Objective To investigate the expression of lncRNA Gas5 in NSCLC and to evaluate its clinical significance.Methods 50 specimens of NSCLC tissues and normal lung tissues after surgery were collected.Quanti-tative real-time polymerase chain reaction (qRT-PCR)for Gas5 expression was performed on cancerous and normal lung tissues.The expression of Gas5 was also analyzed between lung cancer cell lines and bronchial epithelial cells. The correlations between the expression of Gas5 and pathological characteristics were analyzed.Results The expres-sion level of Gas5 was down-regulated in tumor tissues while compared with the paired normal lung tissues (P<0.05 ).The expression of Gas5 was associated with lymphatic metastasis,tumor size and TNM stages (P<0.05 ). The low expression of Gas5 was significantly correlated with overall survival of NSCLC (P<0.05 ).Conclusion The expression of Gas5 is down-regulated in tumor tissues and can serve as a potential prognostic for NSCLC.