临床肺科杂志
臨床肺科雜誌
림상폐과잡지
JOUNAL OF CLINICAL PULMONARY MEDICINE
2015年
2期
204-206
,共3页
潘燕玉%李津%王心静%杨秉芬%程小星
潘燕玉%李津%王心靜%楊秉芬%程小星
반연옥%리진%왕심정%양병분%정소성
脑脊液%ELISPOT%结核性脑膜炎%诊断
腦脊液%ELISPOT%結覈性腦膜炎%診斷
뇌척액%ELISPOT%결핵성뇌막염%진단
cerebrospinal fluid%ELISPOT%tuberculosis meningitis%diagnosis
目的:利用酶联免疫斑点法(ELISPOT)检测脑脊液中结核抗原特异性的INF-γ分泌细胞数量,研究其在结核性脑膜炎诊断中的价值。方法对104例结核性脑膜炎患者和40例非结核性脑膜炎患者进行脑脊液与血的ELISPOT检测,并与脑脊液抗酸染色涂片、结核菌培养、荧光定量PCR、结核抗体检测进行敏感度和特异性的比较。结果脑脊液ELISPOT检测的敏感度为67.3%、特异性为89.2%,PV+值94.3%优于其他检测方法,差别具有统计学意义(P<0.05),脑脊液ELISPOT检测具有较好的诊断价值(Kappa=0.856, P<0.001)。结论脑脊液ELISPOT检测对结核性脑膜炎诊断的敏感度及特异度高,有望成为快速诊断结核性脑膜炎的一种方法。
目的:利用酶聯免疫斑點法(ELISPOT)檢測腦脊液中結覈抗原特異性的INF-γ分泌細胞數量,研究其在結覈性腦膜炎診斷中的價值。方法對104例結覈性腦膜炎患者和40例非結覈性腦膜炎患者進行腦脊液與血的ELISPOT檢測,併與腦脊液抗痠染色塗片、結覈菌培養、熒光定量PCR、結覈抗體檢測進行敏感度和特異性的比較。結果腦脊液ELISPOT檢測的敏感度為67.3%、特異性為89.2%,PV+值94.3%優于其他檢測方法,差彆具有統計學意義(P<0.05),腦脊液ELISPOT檢測具有較好的診斷價值(Kappa=0.856, P<0.001)。結論腦脊液ELISPOT檢測對結覈性腦膜炎診斷的敏感度及特異度高,有望成為快速診斷結覈性腦膜炎的一種方法。
목적:이용매련면역반점법(ELISPOT)검측뇌척액중결핵항원특이성적INF-γ분비세포수량,연구기재결핵성뇌막염진단중적개치。방법대104례결핵성뇌막염환자화40례비결핵성뇌막염환자진행뇌척액여혈적ELISPOT검측,병여뇌척액항산염색도편、결핵균배양、형광정량PCR、결핵항체검측진행민감도화특이성적비교。결과뇌척액ELISPOT검측적민감도위67.3%、특이성위89.2%,PV+치94.3%우우기타검측방법,차별구유통계학의의(P<0.05),뇌척액ELISPOT검측구유교호적진단개치(Kappa=0.856, P<0.001)。결론뇌척액ELISPOT검측대결핵성뇌막염진단적민감도급특이도고,유망성위쾌속진단결핵성뇌막염적일충방법。
Objective To detect antigen-specific INF-γsecreting cells in CSF by ELISPOT assay to deter-mine its clinical value for TBMdiagnosis.Methods The study selected 1 04 patients with tuberculous meningitis and 40 people without tuberculous meningitis to detect their INF-γsecreting cells in CSF and blood by ELISPOT assay. Then the results were compared with AFB staining,Mtb culture,quantitative PCR,and Mtb antibodies.Results The sensitivity of CSF-ELISPOT assay was 92.3%,the specificity was 94.1%,which were higher than other assays (P<0.05).The CSF-ELISPOT assay was better than other assay in the diagnosis of TBM (Kappa=0.856,P<0.001 ).Conclusion The CSF-ELISPOT assay,with high sensitivity and specificity,will be a rapid diagnostic method for TBM.