CAJ | 학술논문

目的：采用结核分枝杆菌特异性IFN-γ酶联免疫斑点（Elispot）检测结核性脑膜炎病人外周血和脑脊液细胞中IFN-γ的表达，评价该方法在结核性脑膜炎诊断中的应用情况。方法采用自建立的结核分枝杆菌特异性IFN-γElispot检测技术（简称Elispot）检测16例结核性脑膜炎病人PBMC和脑脊液中结核菌特异性IFN-γ水平。结果 ELISPOT检测两种细胞分别在ESAT6蛋白和PoolA多肽的作用下，释放的IFN-γ水平均没差别（P＞0.05）；两种标本ELISPOT检测阳性率没有差异（P＞0.05）。结论建立的结核特异性的Elispot检测诊断方法适用于脑脊液标本的检测，并且其检测效果与外周血细胞的检测是基本一致。
목적：채용결핵분지간균특이성IFN-γ매련면역반점（Elispot）검측결핵성뇌막염병인외주혈화뇌척액세포중IFN-γ적표체，평개해방법재결핵성뇌막염진단중적응용정황。방법채용자건립적결핵분지간균특이성IFN-γElispot검측기술（간칭Elispot）검측16례결핵성뇌막염병인PBMC화뇌척액중결핵균특이성IFN-γ수평。결과 ELISPOT검측량충세포분별재ESAT6단백화PoolA다태적작용하，석방적IFN-γ수평균몰차별（P＞0.05）；량충표본ELISPOT검측양성솔몰유차이（P＞0.05）。결론건립적결핵특이성적Elispot검측진단방법괄용우뇌척액표본적검측，병차기검측효과여외주혈세포적검측시기본일치。
Objective To compare the level of IFN-γin blood and cerebrospinal fluid (CSF)of patients with tuberculosis meningitis.Methods The expression of IFN-γin PBMC and CSF was detected in 1 6 TBM patients by Elispot method.Results The level of IFN-γin PBMC and CSF showed no significant difference under the stimu-lation of ESAT-6 and peptide.There was no significant difference in the positive rate of Elispot between PBMC and CSF.Conclusion The result shows that Elispot can be used to detect the expression of IFN-γin CSF of TBM pa-tients,as well as in PMBC.