临床肺科杂志
臨床肺科雜誌
림상폐과잡지
JOUNAL OF CLINICAL PULMONARY MEDICINE
2015年
2期
195-198
,共4页
结核%趋化因子%MCP-1%LTBI
結覈%趨化因子%MCP-1%LTBI
결핵%추화인자%MCP-1%LTBI
tuberculosis%chemokines%MCP-1%LTBI
目的：通过测定外周血单个核细胞（PBMCs）中单核细胞趋化蛋白（MCP-1）的表达水平，探讨其对结核诊断的意义。方法收集活动性肺结核患者、结核潜伏感染（latent tuberculosis infection，LTBI）及非结核感染健康对照个体的全血并提取PBMCs，经结核特异性抗原肽刺激后，采用基因芯片分析方法和酶联免疫吸附试验（ELISA）比较各组MCP-1表达情况。结果基因芯片分析发现活动期肺结核患者MCP-1表达（4460±3154）明显高于LTBI组（951.8±641.5，P＝0.0043）。ELISA分析结果发现刺激前三组MCP-1浓度无差异（P＝0.4747），而刺激后只有活动期肺结核患者组（37112±3790 pg／ml）和非结核感染健康对照组（15546±3143 pg／ml）之间的MCP-1浓度相比有显著差异（P＜0.0001）。结论高表达的MCP-1有可能作为诊断结核及监测结核严重程度的指标之一，但是无法区分潜伏感染和活动期感染病例。
目的：通過測定外週血單箇覈細胞（PBMCs）中單覈細胞趨化蛋白（MCP-1）的錶達水平，探討其對結覈診斷的意義。方法收集活動性肺結覈患者、結覈潛伏感染（latent tuberculosis infection，LTBI）及非結覈感染健康對照箇體的全血併提取PBMCs，經結覈特異性抗原肽刺激後，採用基因芯片分析方法和酶聯免疫吸附試驗（ELISA）比較各組MCP-1錶達情況。結果基因芯片分析髮現活動期肺結覈患者MCP-1錶達（4460±3154）明顯高于LTBI組（951.8±641.5，P＝0.0043）。ELISA分析結果髮現刺激前三組MCP-1濃度無差異（P＝0.4747），而刺激後隻有活動期肺結覈患者組（37112±3790 pg／ml）和非結覈感染健康對照組（15546±3143 pg／ml）之間的MCP-1濃度相比有顯著差異（P＜0.0001）。結論高錶達的MCP-1有可能作為診斷結覈及鑑測結覈嚴重程度的指標之一，但是無法區分潛伏感染和活動期感染病例。
목적：통과측정외주혈단개핵세포（PBMCs）중단핵세포추화단백（MCP-1）적표체수평，탐토기대결핵진단적의의。방법수집활동성폐결핵환자、결핵잠복감염（latent tuberculosis infection，LTBI）급비결핵감염건강대조개체적전혈병제취PBMCs，경결핵특이성항원태자격후，채용기인심편분석방법화매련면역흡부시험（ELISA）비교각조MCP-1표체정황。결과기인심편분석발현활동기폐결핵환자MCP-1표체（4460±3154）명현고우LTBI조（951.8±641.5，P＝0.0043）。ELISA분석결과발현자격전삼조MCP-1농도무차이（P＝0.4747），이자격후지유활동기폐결핵환자조（37112±3790 pg／ml）화비결핵감염건강대조조（15546±3143 pg／ml）지간적MCP-1농도상비유현저차이（P＜0.0001）。결론고표체적MCP-1유가능작위진단결핵급감측결핵엄중정도적지표지일，단시무법구분잠복감염화활동기감염병례。
Objective To evaluate the diagnostic value of MCP-1 for tuberculosis by assaying its expression level in PBMCs.Methods The peripheral blood was collected from pulmonary tuberculosis patients,latent tubercu-losis infection (LTBI)and non-tuberculosis infection healthy individuals.Then MCP-1 level in PBMCs stimulated with Mtb-specific antigens was detected using microarray analysis methods and enzyme-linked immunosorbent assay (ELISA).Results Microarray analysis showed that MCP-1 level in patients with active tuberculosis (4460 ±31 54) was significantly higher than that in the LTBI group (951.8 ±641.5,P=0.0043).ELISA analysis revealed no difference in MCP-1 concentration in PBMCs culture supernatants among the three groups before stimulation (P=0.4747),but there was significant difference in MCP-1 concentration between the active tuberculosis patients group (371 1 2 ±3790 pg/ml)and the healthy control group (1 5546 ±31 43 pg/ml)after stimulation (P<0.0001 ).Con-clusion The high expression of MCP-1 can be used as an indicator for diagnosis of tuberculosis and monitor the dis-ease severity,but it can't distinguish latent infection from active infection.
