国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
24期
3674-3678
,共5页
单核苷酸%多态性%冠心病%Gensini积分
單覈苷痠%多態性%冠心病%Gensini積分
단핵감산%다태성%관심병%Gensini적분
Mononucleotide%Polymorphism%Coronary artery disease%Gensini score
目的 研究CDKN2A/B基因邻近的rs2383206、rs10757274、rs 10757278三个位点的单核苷酸多态性(SNP),探讨其与冠心病的发病及冠状动脉病变程度的相关性.方法 采用聚合酶链式反应(PCR)扩增CDKN2A/B基因邻近rs2383206、rs 10757274、rs 10757278三个SNP位点的核苷酸片段,进行多态性分型并分析其与冠心病发病的相关性,对冠心病患者冠脉造影结果进行Gensini积分评定,分析单核苷酸多态性分型与Gensini积分的关系.结果 CDKN2A/B基因邻近的3个SNP位点rs2383206、rs10757274、rs 10757278的AG/GG基因型分布频率均高于对照组(P＜0.05),G等位基因分布频率高于对照组(P＜0.05).经校正混杂因素的影响后,与AA纯合子相比rs2383206、rs10757274、rs10757278的AG/GG基因型具有显著增加冠心病风险的效应校正比值比(OR)分别为1.492, 1.915,1.866.冠心病组3个SNP位点AG/GG基因型冠脉Gensini积分均高于AA基因型(P＜0.05).结论 CDKN2A/B基因的rs2383206、rs10757274、rs10757278三个单核苷酸多态性位点是昆明地区汉族冠心病发生的易感位点,且与冠状动脉病变的严重程度呈正相关.
目的 研究CDKN2A/B基因鄰近的rs2383206、rs10757274、rs 10757278三箇位點的單覈苷痠多態性(SNP),探討其與冠心病的髮病及冠狀動脈病變程度的相關性.方法 採用聚閤酶鏈式反應(PCR)擴增CDKN2A/B基因鄰近rs2383206、rs 10757274、rs 10757278三箇SNP位點的覈苷痠片段,進行多態性分型併分析其與冠心病髮病的相關性,對冠心病患者冠脈造影結果進行Gensini積分評定,分析單覈苷痠多態性分型與Gensini積分的關繫.結果 CDKN2A/B基因鄰近的3箇SNP位點rs2383206、rs10757274、rs 10757278的AG/GG基因型分佈頻率均高于對照組(P＜0.05),G等位基因分佈頻率高于對照組(P＜0.05).經校正混雜因素的影響後,與AA純閤子相比rs2383206、rs10757274、rs10757278的AG/GG基因型具有顯著增加冠心病風險的效應校正比值比(OR)分彆為1.492, 1.915,1.866.冠心病組3箇SNP位點AG/GG基因型冠脈Gensini積分均高于AA基因型(P＜0.05).結論 CDKN2A/B基因的rs2383206、rs10757274、rs10757278三箇單覈苷痠多態性位點是昆明地區漢族冠心病髮生的易感位點,且與冠狀動脈病變的嚴重程度呈正相關.
목적 연구CDKN2A/B기인린근적rs2383206、rs10757274、rs 10757278삼개위점적단핵감산다태성(SNP),탐토기여관심병적발병급관상동맥병변정도적상관성.방법 채용취합매련식반응(PCR)확증CDKN2A/B기인린근rs2383206、rs 10757274、rs 10757278삼개SNP위점적핵감산편단,진행다태성분형병분석기여관심병발병적상관성,대관심병환자관맥조영결과진행Gensini적분평정,분석단핵감산다태성분형여Gensini적분적관계.결과 CDKN2A/B기인린근적3개SNP위점rs2383206、rs10757274、rs 10757278적AG/GG기인형분포빈솔균고우대조조(P＜0.05),G등위기인분포빈솔고우대조조(P＜0.05).경교정혼잡인소적영향후,여AA순합자상비rs2383206、rs10757274、rs10757278적AG/GG기인형구유현저증가관심병풍험적효응교정비치비(OR)분별위1.492, 1.915,1.866.관심병조3개SNP위점AG/GG기인형관맥Gensini적분균고우AA기인형(P＜0.05).결론 CDKN2A/B기인적rs2383206、rs10757274、rs10757278삼개단핵감산다태성위점시곤명지구한족관심병발생적역감위점,차여관상동맥병변적엄중정도정정상관.
Objective To investigate single nucleotide polymorphism (SNP) of rs2383206,rs 10757274 and rs 10757278 on C DKN2A/B,and study the relationship between SNP and severity of coronary artery disease.Methods PCR-SNP stream technology was applied to determine rs2383206,rs10757274 and rs10757278 neighboring gene CDKN2A/B,in the mean time Gensini score was used to evaluate patients with coronary artery disease.Statistical analysis was done.Results The frequencies of AG/GG genotype of rs2383206,rs10757274 and rs 10757278 gene in coronary artery disease group were significantly higher than those in control group (P ＜ 0.05),so did G allele frequencies (P ＜ 0.05).After adjusting for confounders,compared with AA homozygote,AG/GG genotypes of rs2383206,rs10757274 and rs10757278 were associated with a remarkable increased risk of coronary artery disease (OR =1.492,1.915,1.866).In coronary artery disease group,for those 3 SNP,Gensini scores of patients with AG/GG genotypes were higher than those with AA genotype (P ＜ 0.05).Conclusion SNP of rs2383206,rs10757274 and rs10757278 are the susceptible loci for coronary artery disease in Kunming Hans,which is positively related to the severity of coronary artery disease.
