CAJ | 학술논문

目的建立一套简便易行的活体神经分离和神经放电记录技术.为进一步研究生理和病理条件下呼吸运动神经功能奠定基础.方法 10只SD大鼠,350～450 g,腹腔麻醉,颈部正中切口,于体视显微镜下分离同侧膈神经及舌下神经,分离并切断双侧迷走神经.用神经记录电极引导神经放电,多导生理仪记录放电,应用分析软件对结果进行分析.结果分别以臂丛和二腹肌后腹为标记点,可较顺利分离膈神经和舌下神经.记录60 s,膈神经放电面积(36.3±3.2)μV.s,峰峰值(42.6±8.30) μV,最大值33.5 μV,最小值-19.5 μV;舌下神经放电面积(18.7±4.2) μV.s,峰峰值(33.6±9.30) μV,最大值27.9 μV,最小值一22.0 μV.结论鉴于大鼠神经分离和在体记录技术存在一定手术操作和记录技术的难度,呼吸运动神经功能研究受阻.为此,我们建立了一套简便易行的神经分离和在体神经记录方法,为研究呼吸运动神经放电奠定了基础.
목적 건립일투간편역행적활체신경분리화신경방전기록기술.위진일보연구생리화병리조건하호흡운동신경공능전정기출.방법 10지SD대서,350～450 g,복강마취,경부정중절구,우체시현미경하분리동측격신경급설하신경,분리병절단쌍측미주신경.용신경기록전겁인도신경방전,다도생리의기록방전,응용분석연건대결과진행분석.결과 분별이비총화이복기후복위표기점,가교순리분리격신경화설하신경.기록60 s,격신경방전면적(36.3±3.2)μV.s,봉봉치(42.6±8.30) μV,최대치33.5 μV,최소치-19.5 μV;설하신경방전면적(18.7±4.2) μV.s,봉봉치(33.6±9.30) μV,최대치27.9 μV,최소치일22.0 μV.결론 감우대서신경분리화재체기록기술존재일정수술조작화기록기술적난도,호흡운동신경공능연구수조.위차,아문건립료일투간편역행적신경분리화재체신경기록방법,위연구호흡운동신경방전전정료기출.
Objective To establish a set of explicit method of isolating phrenic and hypoglossal nerve and recording their discharges,which may lay a foundation for further investigating the function of respiratory motor nerve in physiological and pathological conditions.Methods Ten healthy SD rats (350-450 g) were anesthetized intraperitoneally and subject to cervical midline skin incision.The phrenic nerve,the hypoglossal nerve of the same side were isolated with the bilateral vagotomy under the surgical microscope.Two-polar electrodes were connected to the nerves and multi-channel physiological recorder was used to record the electrical activities of nerves.Results Brachial plexus and posterior belly of digastrics muscles are location markers of phrenic and hypoglossal lnerves respectively.For 60 seconds,the area of phrenic discharging was (36.3 ± 3.2) μV,the amplitude was (42.6 ± 8.30) μtV,maximum peak was 33.5 μV and the minimal peak was-19.5 μV.As to the charges of hypoglossal nerve,the discharging area was (18.7±4.2) μV,the amplitude was (33.6±9.30) μV,maximum peak was 27.9μVand the minimal peak was-22.0 μV.Conclusions There are certain difficulties of isolating and recording the phrenic and hypoglossal nerves,which may hinder the study of the function of respiratory motor nerves.The present study established a convenient way to isolat and record charges of respiratory nerve,which provide anatomic basis for further investigation.