CAJ | 학술논문

目的对两种快速、简便能区分诊断恶性疟和间日疟的胶体金免疫层析试条的检测效果进行评价. 方法筛选基于恶性疟原虫富组蛋白Ⅱ和恶性疟原虫乳酸脱氢酶制备的单克隆抗体对,采用柠檬酸三钠还原法制备胶体金颗粒,标记筛选到的单克隆抗体C6E9和G4C9,并将其同时吸附于样品垫;将单克隆抗体B2G10(针对恶性疟原虫与间日疟原虫)和C6H.(只针对恶性疟原虫)分别划线包被于同一硝酸纤维素膜适当位置,制成免疫层析检测试条.用该试条检测疫区非疟疾发热病人血样(120份)和内脏利什曼病患者血样(20份)以评价其特异性,检测确诊的疟疾患者血样(间日疟105份,恶性疟95份)以评价其敏感性.均用单盲法检测,同时用基于恶性疟原虫乳酸脱氢酶制备的单克隆抗体对制备的试条进行平行检测. 结果检测120份疫区非疟疾发热病人血样和20份内脏利什曼病患者血样,有138份显示为阴性,特异性为98.6％,其中20份内脏利什曼病患者血样全部为阴性.检测200份疟疾患者血样,阳性190份,敏感性为95.0％,其中间日疟检出率为93.3％ (98/105),恶性疟检出率为96.8％ (92/95).基于恶性疟原虫乳酸脱氢酶制备的单克隆抗体对制备的试条检测的敏感性和特异性分别为93.5％ (187/200)和95.7％ (134/140),与本研究制备的基于恶性疟原虫富组蛋白Ⅱ和恶性疟原虫乳酸脱氢酶制备的单克隆抗体对的试条比较两者敏感性(x2=0.42,P＞0.05)和特异性(x2=1.09,P＞0.05)的差异均无统计学意义. 结论研制出的基于恶性疟原虫富组蛋白Ⅱ和恶性疟原虫乳酸脱氢酶制备的单克隆抗体对的快速诊断疟疾胶体金免疫层析试条敏感性、特异性均较高.
목적 대량충쾌속、간편능구분진단악성학화간일학적효체금면역층석시조적검측효과진행평개. 방법 사선기우악성학원충부조단백Ⅱ화악성학원충유산탈경매제비적단극륭항체대,채용저몽산삼납환원법제비효체금과립,표기사선도적단극륭항체C6E9화G4C9,병장기동시흡부우양품점;장단극륭항체B2G10(침대악성학원충여간일학원충)화C6H.(지침대악성학원충)분별화선포피우동일초산섬유소막괄당위치,제성면역층석검측시조.용해시조검측역구비학질발열병인혈양(120빈)화내장리십만병환자혈양(20빈)이평개기특이성,검측학진적학질환자혈양(간일학105빈,악성학95빈)이평개기민감성.균용단맹법검측,동시용기우악성학원충유산탈경매제비적단극륭항체대제비적시조진행평행검측. 결과 검측120빈역구비학질발열병인혈양화20빈내장리십만병환자혈양,유138빈현시위음성,특이성위98.6％,기중20빈내장리십만병환자혈양전부위음성.검측200빈학질환자혈양,양성190빈,민감성위95.0％,기중간일학검출솔위93.3％ (98/105),악성학검출솔위96.8％ (92/95).기우악성학원충유산탈경매제비적단극륭항체대제비적시조검측적민감성화특이성분별위93.5％ (187/200)화95.7％ (134/140),여본연구제비적기우악성학원충부조단백Ⅱ화악성학원충유산탈경매제비적단극륭항체대적시조비교량자민감성(x2=0.42,P＞0.05)화특이성(x2=1.09,P＞0.05)적차이균무통계학의의. 결론 연제출적기우악성학원충부조단백Ⅱ화악성학원충유산탈경매제비적단극륭항체대적쾌속진단학질효체금면역층석시조민감성、특이성균교고.
Objective To evaluate the effect of two gold immunochromatographic strip tests for detection and differentiation of Plasmodium vivax and P.falciparum.Methods The monoclonal antibodies were screened for detection and differentiation of P.vivax and P.falciparum based on the P.falciparum histidinerich protein Ⅱ (HRP-Ⅱ) and P.falciparum lactate dehydrogenase(LDH).Firstly,the monoclonal antibodies,C6E9 and G4C9,were conjugated with colloid gold as detecting reagent.Secondly,the monoclonal antibodies B2G10 (targeting on P.vivax/P.faciparum) and C6H11 (only targeting on P.falciparum) were immobilized on same nitrocellulose in proper position.Blood samples from 120 febrile patients without malaria infection from endemic area of malaria and 20 patients with visceral leishmaniasis were used for evaluating the specificity of the tests.Blood samples of malaria patients(105 with P.vivax and 95 with P.falciparum) were used for evaluating the sensitivity of the tests.The results of our immunochromatographic strip were compared with that of the immunochromatographic strip based on the monoclonal antibodies of LDH.Results Only two samples out of 120 febrile patients and 20 patients with visceral leishmaniasis showed false positive reaction with a specificity of 98.6％(138/140),moreover,all the 20 samples from patients with visceral leishmaniasis were negative.Two-hundred blood samples of malaria patients showed a sensitivity of 95.0％(190/200) totally,and sensitivities of 93.3％(98/105) and 96.8％(92/95) for patients infected with P.vivax or P.falciparum,respectively.The sensitivity and specificity of the immunochromatographic strip based on the monoclonal antibodies of LDH were 93.5％(187/200) and 95.7％ (134/140),respectively.Both sensitivity and specificity showed no statistical difference between the immunochromatographic strip based on the monoclonal antibodies of LDH and the immunochromatographic strip based on the monoclonal antibodies of HRP-Ⅱ and LDH.Conclusion The immunochromatographic strip test based on the monoclonal antibodies of HRP-Ⅱ and LDH is a sensitive,specific,simple and rapid assay for malaria diagnosis.