CAJ | 학술논문

目的阐明海南岛某些按蚊的分类地位,探讨迷走按蚊与其近缘种的亲缘关系. 方法对采自海南岛8个地点的按蚊依据形态和分子特征进行鉴别研究,应用的分子特征包括核糖体DNA(ribosomal DNA,rDNA)第2内转录间隔区(second internal transcribed spacer,ITS2)和28S第3编码区(third domain,D3)序列,综合分析迷走按蚊及其近缘种的ITS2和D3序列,构建系统发育树. 结果本研究共鉴定按蚊407只,其中84.9％的成蚊分子鉴定与依据形态鉴定的结果一致.在228条迷走按蚊的ITS2序列中,部分个体在2个位点存在单碱基套峰(G/A),出现的频率分别为57.9％、64.8％,同时有两个套峰的占样本数的57.0％,其他位点保守.迷走按蚊的D3序列,在种内无碱基差异.结合本研究和文献的迷走按蚊及其近缘种ITS2序列构建的ML树,显示迷走按蚊与浅色按蚊MC/D亲缘关系近,与另一支的浅色按蚊B、圣代克按蚊复合体的亲缘关系较远.关于未订名种NBC (Anophelessp.NBG)的分类地位,依据分子特征鉴定其为浅色按蚊B,可能是圣代克按蚊复合体新的成员种.结论鉴定形态在个体间变异大的种类时,客观的分子特征更具重要性,迷走按蚊与浅色按蚊MC/D具较近的亲缘关系.
목적 천명해남도모사안문적분류지위,탐토미주안문여기근연충적친연관계. 방법 대채자해남도8개지점적안문의거형태화분자특정진행감별연구,응용적분자특정포괄핵당체DNA(ribosomal DNA,rDNA)제2내전록간격구(second internal transcribed spacer,ITS2)화28S제3편마구(third domain,D3)서렬,종합분석미주안문급기근연충적ITS2화D3서렬,구건계통발육수. 결과 본연구공감정안문407지,기중84.9％적성문분자감정여의거형태감정적결과일치.재228조미주안문적ITS2서렬중,부분개체재2개위점존재단감기투봉(G/A),출현적빈솔분별위57.9％、64.8％,동시유량개투봉적점양본수적57.0％,기타위점보수.미주안문적D3서렬,재충내무감기차이.결합본연구화문헌적미주안문급기근연충ITS2서렬구건적ML수,현시미주안문여천색안문MC/D친연관계근,여령일지적천색안문B、골대극안문복합체적친연관계교원.관우미정명충NBC (Anophelessp.NBG)적분류지위,의거분자특정감정기위천색안문B,가능시골대극안문복합체신적성원충.결론 감정형태재개체간변이대적충류시,객관적분자특정경구중요성,미주안문여천색안문MC/D구교근적친연관계.
Objective To clarify the taxonomic status of anopheline mosquitoes in Hainan Island and reconstruct the molecular phylogenetic relationship among Anopheles vagus and its cryptic species.Methods Anopheline mosquitoes from 8 sites in Hainan Island were distinguished based on morphology and molecular markers,with ribosomal DNA second internal transcribed spacer (rDNA-ITS2)and the third domain of 28S (28S-D3) sequences.The phylogenetic tree,using maximum likelihood method,was reconstructed by rDNA-ITS2 and 28S-D3 dataset,after the sequences of mosquito species within Pyretophorus series were analyzed.Results Total 407 individuals were identified in this study.The agreement between results of molecular identification and morphology in adults was 84.9％.In 228 ITS2 sequences of An.vagus,some individuals at two positions exhibitd double-peaks (G/A),the frequencies were 57.9％,64.8％,respectively,both for 57％.Other positions were intraspecific conservation.There were no differences of D3 sequences within An.vagus individuals.The ML tree based on ITS2 sequences showed that An.vagus and An.subpictus cytotype A/C/D were close on the same clade,while far away with the other clades with An.subpictus cytotype B and An.sundiacus complex.Anopheles sp.NBG was An.subpictus cytotype B by ITS2 marker.However,it was also likely a new member of An.sundiacus complex.Conclusion Molecular marker is more important in distinguishing anopheline mosquitoes species while there are many intraspecific morphology variations.The relationship between An.vagus and An.subpictus cytotype A/C/D is closer than that between subpictus cytotype B and An.subpictus cytotype A/C/D.