CAJ | 학술논문

目的探讨小动脉闭塞性卒中(small artery occlusion,SAO)患者与同期健康体检者血浆微小RNA(microRNAs,miRNAs)表达谱差异.方法选取TOAST分型为小动脉闭塞性卒中患者8例,以8例同期健康体检者作为对照组,应用高通量测序技术检测血浆miRNAs表达谱,筛选出差异表达的miRNAs,采用实时荧光定量聚合酶链反应验证结果,并进行靶基因预测和生物信息学分析.结果 miRNAs差异化分析显示,SAO组miRNA-127、miRNA-99b-5p和miRNA-320等19个miRNAs 较对照组表达显著性上调(P均＜0.01),而miRNA-451a等5个miRNAs较对照组表达显著性下调(P均＜0.01).实时荧光定量聚合酶链反应对其中miRNA-127、miRNA-99b-5p、miRNA-320和miRNA-451a的验证结果与高通量测序结果一致.生物信息学分析显示,差异化表达的miRNAs调控的靶基因主要与细胞增殖、黏附、系统发育、高分子代谢等生物学功能相关.结论 SAO患者与健康体检者血浆miRNAs表达谱存在显著性差异,提示miRNAs可能通过靶基因在SAO的发病过程中发挥调节作用.
목적 탐토소동맥폐새성졸중(small artery occlusion,SAO)환자여동기건강체검자혈장미소RNA(microRNAs,miRNAs)표체보차이.방법 선취TOAST분형위소동맥폐새성졸중환자8례,이8례동기건강체검자작위대조조,응용고통량측서기술검측혈장miRNAs표체보,사선출차이표체적miRNAs,채용실시형광정량취합매련반응험증결과,병진행파기인예측화생물신식학분석.결과 miRNAs차이화분석현시,SAO조miRNA-127、miRNA-99b-5p화miRNA-320등19개miRNAs 교대조조표체현저성상조(P균＜0.01),이miRNA-451a등5개miRNAs교대조조표체현저성하조(P균＜0.01).실시형광정량취합매련반응대기중miRNA-127、miRNA-99b-5p、miRNA-320화miRNA-451a적험증결과여고통량측서결과일치.생물신식학분석현시,차이화표체적miRNAs조공적파기인주요여세포증식、점부、계통발육、고분자대사등생물학공능상관.결론 SAO환자여건강체검자혈장miRNAs표체보존재현저성차이,제시miRNAs가능통과파기인재SAO적발병과정중발휘조절작용.
Objective To investigate the difference of expression profiling of plasma miRNAs (microRNA) between the patients with small artery occlusive stroke (SAO) and the healthy subjects.Methods Eight patients with SAO classified by TOAST were selected and 8 healthy subjects were used as a control group.High-throughput sequencing technology was used to detect the expression profiling of plasma miRNAs.The differentially expressed miRNAs were screened.Real-time fluorescent quantitative polymerase chain reaction was used to validate the results,and the target gene prediction and bioinformatics analysis were performed.Results The miRNA difference analysis showed that the expression profilings of miRNA-127,miRNA-99b-5p,miRNA-320,and other 19 miRNAs in the SAO group were significantly upregulated compared with those in the control group (all P＜0.01),while miRNA-451a and other 5 miRNAs in the SAO group were significantly downregulated compared with those in the control group (all P ＜0.01).The validated results of miRNA-127,miRNA-99b-5p,miRNA-320,and miRNA-451a with real-time fluorescent quantitative polymerase chain reaction were consistent with those of the high-throughput sequencing.Bioinformatics analysis showed that the miRNA-regulated target genes expressed differentially were mainly correlated with cell proliferation,adhesion,phylogenetic development,macromolecule metabolism,and other biological functions.Conclusions There are significant differences in the expression profiling of plasma miRNAs between the patients with SAO and the healthy subjects,suggesting that miRNAs may play a regulatory role via target genes in pathogenesis of SAO.