CAJ | 학술논문

目的探讨寻常性银屑病患者外周血Th22细胞及相关因子白细胞介素22(IL-22)表达水平与疾病严重程度及病程的相关性.方法抽取40例寻常性银屑病患者和30例健康对照者外周血,流式细胞仪检测外周血Th22细胞比例,ELISA法检测血清中IL-22的表达.采用t检验、Pearson相关分析法进行分析.结果寻常性银屑病患者外周血Th22细胞百分比(0.65％±0.48％)高于健康对照组(0.33％±0.15％),两组差异有统计学意义(t=3.89,P＜ 0.01);银屑病患者Th22细胞与银屑病病情严重度评分(PASI)呈正相关(r=0.38,P＜ 0.05),但与病程无相关性(r=0.20,P＞ 0.05).银屑病患者血清中IL-22表达水平[(67.96±14.32) ng/L]高于健康对照组[(40.59±9.91)ng/L],两组差异有统计学意义(t=9.45,P＜0.01);银屑病患者血清IL-22表达水平与PASI呈正相关(r=0.94,P＜ 0.01),与病程无相关性(r=0.10,P＞ 0.05).结论 Th22细胞及IL-22在寻常性银屑病外周血中有高表达,且Th22细胞及IL-22水平均与疾病严重程度相关.
목적 탐토심상성은설병환자외주혈Th22세포급상관인자백세포개소22(IL-22)표체수평여질병엄중정도급병정적상관성.방법 추취40례심상성은설병환자화30례건강대조자외주혈,류식세포의검측외주혈Th22세포비례,ELISA법검측혈청중IL-22적표체.채용t검험、Pearson상관분석법진행분석.결과 심상성은설병환자외주혈Th22세포백분비(0.65％±0.48％)고우건강대조조(0.33％±0.15％),량조차이유통계학의의(t=3.89,P＜ 0.01);은설병환자Th22세포여은설병병정엄중도평분(PASI)정정상관(r=0.38,P＜ 0.05),단여병정무상관성(r=0.20,P＞ 0.05).은설병환자혈청중IL-22표체수평[(67.96±14.32) ng/L]고우건강대조조[(40.59±9.91)ng/L],량조차이유통계학의의(t=9.45,P＜0.01);은설병환자혈청IL-22표체수평여PASI정정상관(r=0.94,P＜ 0.01),여병정무상관성(r=0.10,P＞ 0.05).결론 Th22세포급IL-22재심상성은설병외주혈중유고표체,차Th22세포급IL-22수평균여질병엄중정도상관.
Objective To determine the frequency of T helper type 22 (Th22) cells and expression level of interleukin-22 (IL-22) in peripheral blood of patients with psoriasis vulgaris,and to investigate their relationship with disease severity and clinical course.Methods Peripheral blood samples were obtained from 40 patients with psoriasis vulgaris and 30 healthy human controls.Five-color flow cytometry was performed to determine the percentage of Th22 cells in peripheral blood,and enzyme-linked immunosorbent assay (ELISA) to measure the expression of serum IL-22.Statistical analysis was carried out by t test and Pearson correlation analysis.Results Both the percentage of Th22 cells and serum level of IL-22 in peripheral blood were significantly higher in patients with psoriasis vulgaris than in healthy human controls (Th22 cells:0.65％ ± 0.48％ vs.0.33％ ± 0.15％,t =3.89,P ＜ 0.01; IL-22:(67.96 ± 14.32) vs.(40.59 ± 9.91) ng/L,t =9.45,P ＜ 0.01).Further more,Th22 cell percentage and IL-22 serum level in peripheral blood were both positively correlated with psoriasis area and severity index (PASI) in these patients (r =0.38,0.94,P ＜ 0.05 and 0.01,respectively),but neither of them correlated with clinical course of psoriasis vulgaris (r =0.20,0.10,respectively,both P ＞ 0.05).Conclusions The percentage of Th22 cells and level of IL-22 are increased in peripheral blood of patients with psoriasis vulgaris,and both of them are correlated with disease severity.