中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2014年
6期
609-614
,共6页
氟中毒%骨%骨骼微环境
氟中毒%骨%骨骼微環境
불중독%골%골격미배경
Fluorosis%Bone%Bone micro environment
目的 探讨转化生长因子β1 (transforming growth factor-β1,TGF-β1)、白细胞介素6(interleukin,IL-6)在慢性氟中毒大鼠骨组织表达变化的意义.方法 采用随机数字表法,按体质量将36只健康SD大鼠分为3组,每组12只,雌雄各半.对照组饮用自来水(含氟量＜1 mg/L),低氟组和高氟组分别饮用含5、50 mg/L氟化钠的自来水.大鼠饲养6个月,建立慢性氟中毒模型,股动脉放血处死.取大鼠股骨干骺端,灰化-氟离子选择电极法检测骨氟含量;光镜观察骨组织形态学改变;酶联免疫吸附法检测血清骨碱性磷酸酶(BALP)水平;原位杂交和免疫组织化学检测骨组织中TGF-β1和IL-6 mRNA和蛋白表达水平.结果 对照组、低氟组和高氟组的骨氟含量[(306.04±12.57)、(652.91±51.83)、(1 094.11±91.41)mg/kg]依次增加(F=31.14,P＜0.05),低氟组和高氟组大鼠股骨干骺端呈骨质硬化表现.对照组、低氟组和高氟组血清BALP水平[(27.78±4.09)、(46.59±5.75)、(57.45±3.99) U/L]、骨组织TGF-β1 mRNA表达水平(111.84±4.62、123.86±7.46、140.83±5.21)和蛋白表达水平(118.60±7.09、133.17±7.33、145.67±9.61)均依次增加(F值分别为30.29、73.28、33.65,P均＜0.05);低氟组和高氟组骨组织IL-6的mRNA表达水平(117.78±7.01、119.90±5.10)和蛋白表达水平(122.79±6.49、123.81±7.99)均高于对照组(106.49±6.76,112.11±5.80,F值分别为15.47、10.83,P均＜.0.05).结论 TGF-β1、IL-6在染氟大鼠骨组织上表达改变,提示氟可通过调节骨骼微环境来影响骨形成增强.
目的 探討轉化生長因子β1 (transforming growth factor-β1,TGF-β1)、白細胞介素6(interleukin,IL-6)在慢性氟中毒大鼠骨組織錶達變化的意義.方法 採用隨機數字錶法,按體質量將36隻健康SD大鼠分為3組,每組12隻,雌雄各半.對照組飲用自來水(含氟量＜1 mg/L),低氟組和高氟組分彆飲用含5、50 mg/L氟化鈉的自來水.大鼠飼養6箇月,建立慢性氟中毒模型,股動脈放血處死.取大鼠股骨榦骺耑,灰化-氟離子選擇電極法檢測骨氟含量;光鏡觀察骨組織形態學改變;酶聯免疫吸附法檢測血清骨堿性燐痠酶(BALP)水平;原位雜交和免疫組織化學檢測骨組織中TGF-β1和IL-6 mRNA和蛋白錶達水平.結果 對照組、低氟組和高氟組的骨氟含量[(306.04±12.57)、(652.91±51.83)、(1 094.11±91.41)mg/kg]依次增加(F=31.14,P＜0.05),低氟組和高氟組大鼠股骨榦骺耑呈骨質硬化錶現.對照組、低氟組和高氟組血清BALP水平[(27.78±4.09)、(46.59±5.75)、(57.45±3.99) U/L]、骨組織TGF-β1 mRNA錶達水平(111.84±4.62、123.86±7.46、140.83±5.21)和蛋白錶達水平(118.60±7.09、133.17±7.33、145.67±9.61)均依次增加(F值分彆為30.29、73.28、33.65,P均＜0.05);低氟組和高氟組骨組織IL-6的mRNA錶達水平(117.78±7.01、119.90±5.10)和蛋白錶達水平(122.79±6.49、123.81±7.99)均高于對照組(106.49±6.76,112.11±5.80,F值分彆為15.47、10.83,P均＜.0.05).結論 TGF-β1、IL-6在染氟大鼠骨組織上錶達改變,提示氟可通過調節骨骼微環境來影響骨形成增彊.
목적 탐토전화생장인자β1 (transforming growth factor-β1,TGF-β1)、백세포개소6(interleukin,IL-6)재만성불중독대서골조직표체변화적의의.방법 채용수궤수자표법,안체질량장36지건강SD대서분위3조,매조12지,자웅각반.대조조음용자래수(함불량＜1 mg/L),저불조화고불조분별음용함5、50 mg/L불화납적자래수.대서사양6개월,건립만성불중독모형,고동맥방혈처사.취대서고골간후단,회화-불리자선택전겁법검측골불함량;광경관찰골조직형태학개변;매련면역흡부법검측혈청골감성린산매(BALP)수평;원위잡교화면역조직화학검측골조직중TGF-β1화IL-6 mRNA화단백표체수평.결과 대조조、저불조화고불조적골불함량[(306.04±12.57)、(652.91±51.83)、(1 094.11±91.41)mg/kg]의차증가(F=31.14,P＜0.05),저불조화고불조대서고골간후단정골질경화표현.대조조、저불조화고불조혈청BALP수평[(27.78±4.09)、(46.59±5.75)、(57.45±3.99) U/L]、골조직TGF-β1 mRNA표체수평(111.84±4.62、123.86±7.46、140.83±5.21)화단백표체수평(118.60±7.09、133.17±7.33、145.67±9.61)균의차증가(F치분별위30.29、73.28、33.65,P균＜0.05);저불조화고불조골조직IL-6적mRNA표체수평(117.78±7.01、119.90±5.10)화단백표체수평(122.79±6.49、123.81±7.99)균고우대조조(106.49±6.76,112.11±5.80,F치분별위15.47、10.83,P균＜.0.05).결론 TGF-β1、IL-6재염불대서골조직상표체개변,제시불가통과조절골격미배경래영향골형성증강.
Objective To investigate the significance of osteo-immunology related factor transforming growth factor-β1 (TGF-β1) and interleukin 6 (IL-6) in bone of rats with chronic fluorosis.Methods Thirty-six healthy SD rats were divided to three groups according to body weight with the method of random digits table.The rats of control were fed with tap water(NaF ＜ 1 mg/L) and the experimental rats were exposed to NaF (lower dose group:5 mg/L,higher dose group:50 rmg/L) added to the drinking water to establish the chronic fluorosis model.All rats were killed on the six months and the metaphysic of femoral was collected.Bone fluorine was detected by ashing-fluorin ion selective electrode method.Bone tissues were stained with hematoxylin-eosin and observed under optical microscope.The content of bone alkaline phosphatase (BALP) in rat serum was detected by enzyme-linked immunosorbent assay(ELISA).The expressions of TGF-β1 and IL-6 mRNA and protein in bone were detected by in situ hybridization (ISH) and immunohistochemistry (IHC).Results The contents of bone fluorine were increased gradually in the control,the lower and higher doses fluoride groups[(306.04 ± 12.57),(652.91 ± 51.83),(1 094.11 ± 91.41)mg/kg,F =31.14,P ＜ 0.05].Bone sclerosis could be observed under optical microscope in lower and higher dose groups.The content of BALP in serum increased with the dose of fluoride gradually in the control,the lower and higher doses fluoride groups[(27.78 ± 4.09),(46.59 ± 5.75),(57.45 ± 3.99)U/L],expressions of mRNA (111.84 ± 4.62,123.86 ± 7.46,140.83 ± 5.21) and protein (118.60 ± 7.09,133.17 ± 7.33,145.67 ± 9.61) of TGF-β1 were both increased(F =30.29,73,28,33.65,all P ＜ 0.05).The expressions of mRNA(117.78 ± 7.01,119.90 ± 5.10) and protein(122.79 ± 6.49,123.81 ± 7.99) of IL-6 were both higher than those of the control (106.49 ± 6.76,112.11 ± 5.80,F =15.47、10.83,all P ＜ 0.05).Conclusion The expressions of osteo-immunology related factor TGF-β1 and IL-6 in bone of rats with chronic fluorosis have changed,which indicates that fluoride can impact the increased bone formation by regulating the micro environment of bone.
