国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2014年
6期
269-271
,共3页
容新宗%郑朝共%黄琰%许锬%吕家成
容新宗%鄭朝共%黃琰%許錟%呂傢成
용신종%정조공%황염%허담%려가성
哺乳动物呼肠孤病毒3%致细胞病变,病毒%蚀斑形成法
哺乳動物呼腸孤病毒3%緻細胞病變,病毒%蝕斑形成法
포유동물호장고병독3%치세포병변,병독%식반형성법
Mammalian orthoreovirus 3%Cytopathogenic effect,viral%Plaque forming assay
目的 制备高滴度呼肠孤病毒3型(reovirus 3,Reo-3)病毒液,并检测Reo-3滴度.方法 以幼仓鼠肾细胞(BHK-21)为病毒培养基质和滴度测定细胞.将Reo-3按100至10-5感染复数(MOI)接种BHK-21,再分别用细胞病变法和蚀斑形成法检测病毒,用Karber法计算病毒滴度.结果 BHK-21感染Reo-3后能产生明显病变.以10-4 MOI的Reo-3接种后48 h,收获液中病毒滴度最高,细胞病变法检测为9.625 lgTCID50/ml,蚀斑形成法检测为8.671 lgPFU/ml.结论 制备了高滴度Reo-3,为开展该病毒去除灭活研究奠定了基础.
目的 製備高滴度呼腸孤病毒3型(reovirus 3,Reo-3)病毒液,併檢測Reo-3滴度.方法 以幼倉鼠腎細胞(BHK-21)為病毒培養基質和滴度測定細胞.將Reo-3按100至10-5感染複數(MOI)接種BHK-21,再分彆用細胞病變法和蝕斑形成法檢測病毒,用Karber法計算病毒滴度.結果 BHK-21感染Reo-3後能產生明顯病變.以10-4 MOI的Reo-3接種後48 h,收穫液中病毒滴度最高,細胞病變法檢測為9.625 lgTCID50/ml,蝕斑形成法檢測為8.671 lgPFU/ml.結論 製備瞭高滴度Reo-3,為開展該病毒去除滅活研究奠定瞭基礎.
목적 제비고적도호장고병독3형(reovirus 3,Reo-3)병독액,병검측Reo-3적도.방법 이유창서신세포(BHK-21)위병독배양기질화적도측정세포.장Reo-3안100지10-5감염복수(MOI)접충BHK-21,재분별용세포병변법화식반형성법검측병독,용Karber법계산병독적도.결과 BHK-21감염Reo-3후능산생명현병변.이10-4 MOI적Reo-3접충후48 h,수획액중병독적도최고,세포병변법검측위9.625 lgTCID50/ml,식반형성법검측위8.671 lgPFU/ml.결론 제비료고적도Reo-3,위개전해병독거제멸활연구전정료기출.
Objective To prepare high-titer reovirus 3 (Reo-3) and determine its titer.Methods Baby hamster kidney cell (BHK-21) was used for virus cultivation and titration.The cells were infected with 100 to 10-5 multiplicity of infection (MOI) of Reo-3.The virus was detected by cytopathic effect (CPE) method and plaque forming assay,respectively.Virus titers were calculated with Karber formula.Results The CPE on infected BHK-21 cells was easily observed.The highest virus titers,9.625 lgTCID50/ml tested by CPE method and 8.671 lgPFU/ml tested by plaque forming assay,were obtained when BHK-21 cells were inoculated with 104 MOI of Reo-3 48 h after infection.Conclusion High titer Reo-3 can be prepared,which lays the foundation for study on virus inactivation and removal.
