国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2014年
6期
276-280
,共5页
郭蓉%卢佳丽%薛红刚%胡菁%杨燕珠
郭蓉%盧佳麗%薛紅剛%鬍菁%楊燕珠
곽용%로가려%설홍강%호정%양연주
色谱法,离子交换%奈瑟球菌,脑膜炎,血清A群%疫苗,结合%多糖类,细菌
色譜法,離子交換%奈瑟毬菌,腦膜炎,血清A群%疫苗,結閤%多糖類,細菌
색보법,리자교환%내슬구균,뇌막염,혈청A군%역묘,결합%다당류,세균
Chromatography,anion exchange%Neisseria meningitidis,serogroup A%Vaccine,conjugate%Polysaccharides,bacterial
目的 采用高效阴离子交换色谱-脉冲安培法(high-performance anion-exchange chromatography with pulsed amperometric detection,HPAEC-PAD)检测A群脑膜炎球菌多糖-破伤风类毒素结合疫苗(group A meningococcal polysaccharide-tetanus toxoid conjugate vaccine,MenA-PS-TT)中的游离多糖含量.方法 采用1％脱氧胆酸钠(sodium deoxycholate,DOC)处理MenA-PS-TT分离游离PS.游离和结合PS经三氟乙酸水解后,用HPAEC-PAD检测水解产物,并将检测结果与改良钼酸铵分光光度法的检测结果进行比较.结果 1％DOC处理的样品的PS回收率为90％～104％,TT沉淀率为99％以上,说明1％DOC处理不会对游离和结合PS产生影响.HPAEC-PAD检测MenA-PS的重复性良好,3次MenA-PS标准品定位结果和3次MenA-PS-TT成品检测结果的相对标准偏差分别为1.7％和6.4％.HPAEC-PAD与改良钼酸铵分光光度法的检测结果相当.结论 HPAEC-PAD可用于MenA-PS-TT中的游离PS含量检测.
目的 採用高效陰離子交換色譜-脈遲安培法(high-performance anion-exchange chromatography with pulsed amperometric detection,HPAEC-PAD)檢測A群腦膜炎毬菌多糖-破傷風類毒素結閤疫苗(group A meningococcal polysaccharide-tetanus toxoid conjugate vaccine,MenA-PS-TT)中的遊離多糖含量.方法 採用1％脫氧膽痠鈉(sodium deoxycholate,DOC)處理MenA-PS-TT分離遊離PS.遊離和結閤PS經三氟乙痠水解後,用HPAEC-PAD檢測水解產物,併將檢測結果與改良鉬痠銨分光光度法的檢測結果進行比較.結果 1％DOC處理的樣品的PS迴收率為90％～104％,TT沉澱率為99％以上,說明1％DOC處理不會對遊離和結閤PS產生影響.HPAEC-PAD檢測MenA-PS的重複性良好,3次MenA-PS標準品定位結果和3次MenA-PS-TT成品檢測結果的相對標準偏差分彆為1.7％和6.4％.HPAEC-PAD與改良鉬痠銨分光光度法的檢測結果相噹.結論 HPAEC-PAD可用于MenA-PS-TT中的遊離PS含量檢測.
목적 채용고효음리자교환색보-맥충안배법(high-performance anion-exchange chromatography with pulsed amperometric detection,HPAEC-PAD)검측A군뇌막염구균다당-파상풍류독소결합역묘(group A meningococcal polysaccharide-tetanus toxoid conjugate vaccine,MenA-PS-TT)중적유리다당함량.방법 채용1％탈양담산납(sodium deoxycholate,DOC)처리MenA-PS-TT분리유리PS.유리화결합PS경삼불을산수해후,용HPAEC-PAD검측수해산물,병장검측결과여개량목산안분광광도법적검측결과진행비교.결과 1％DOC처리적양품적PS회수솔위90％～104％,TT침정솔위99％이상,설명1％DOC처리불회대유리화결합PS산생영향.HPAEC-PAD검측MenA-PS적중복성량호,3차MenA-PS표준품정위결과화3차MenA-PS-TT성품검측결과적상대표준편차분별위1.7％화6.4％.HPAEC-PAD여개량목산안분광광도법적검측결과상당.결론 HPAEC-PAD가용우MenA-PS-TT중적유리PS함량검측.
Objective To detect free polysaccharide contents in group A meningococcal polysaccharide tetanus toxoid conjugate vaccine (MenA-PS-TT) by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD).Methods The free PSs were separated from MenA-PS-TT using 1％ sodium deoxycholate (DOC).The free and conjugated PSs were hydrolyzed with trifluoroacetic acid,and the hydrolyzates were detected by HPAEC-PAD.The results obtained by HPAEC-PAD were compared with those by improved ammonium molybdate spectrophotometric method.Results After the samples were treated with 1％ DOC,the recovery rates of PS were 90％-104％,and the precipitation rates of TT were above 99％.The study showed that 1％ DOC treatment had no effect on free and conjugated PSs.HPAEC-PAD for determination of MenA-PS had good repeatability,and relative standard deviations of positioning results of MenA-PS standard and detecting results of ManAPS-TT final products for 3 times were 1.7％ and 6.4％,respectively.The results of HPAEC-PAD and improved ammonium molybdate spectrophotometric method were similar.Conclusion HPAEC-PAD can be applied for determination of free PS contents in MenA-PS-TT.
