中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2014年
11期
1138-1141
,共4页
卫政%武永新%柳丽娜%吉鹏娟%申晓彧
衛政%武永新%柳麗娜%吉鵬娟%申曉彧
위정%무영신%류려나%길붕연%신효욱
瑞舒伐他汀%氧化修饰低密度脂蛋白%肝X受体%小凹蛋白1%胆固醇逆转运
瑞舒伐他汀%氧化脩飾低密度脂蛋白%肝X受體%小凹蛋白1%膽固醇逆轉運
서서벌타정%양화수식저밀도지단백%간X수체%소요단백1%담고순역전운
Rosuvastatin%Oxidized low density lipoprotein%Liver X receptor%Caveolin-1%Reverse cholesterol transport
目的 研究不同剂量瑞舒伐他汀对氧化修饰低密度脂蛋白(ox-LDL)诱导的人单核巨噬细胞肝X受体及小凹蛋白1表达的影响.方法 实验分6组:空白对照组、ox-LDL对照组、不同剂量(0.01μmol/L,0.1μmol/L,1.0μmol/L,5.0 μmol/L)瑞舒伐他汀组,RT-PCR法测定各组肝X受体及小凹蛋白1mRNA的表达.结果 空白对照组与ox-LDL对照组肝X受体mRNA的表达分别为1.00±0.02与0.26±0.02,两组比较差异有统计学意义(t=56.392,P＜0.001);小凹蛋白1mRNA分别为1.00±0.04与0.27±0.01,两组比较差异有统计学意义(t=31.278,P＜0.001).ox-LDL对照组及不同剂量瑞舒伐他汀组间肝X受体及小凹蛋白1mRNA表达比较,差异均有统计学意义(F值分别为72.154、66.007,P均＜0.001),随瑞舒伐他汀剂量的增加,肝X受体及小凹蛋白1mRNA表达呈上升趋势,两两比较差异均有统计学意义(P均＜0.05).结论 瑞舒伐他汀可上调单核-巨噬细胞肝X受体mRNA、小凹蛋白1mRNA的表达,且呈剂量依赖性.
目的 研究不同劑量瑞舒伐他汀對氧化脩飾低密度脂蛋白(ox-LDL)誘導的人單覈巨噬細胞肝X受體及小凹蛋白1錶達的影響.方法 實驗分6組:空白對照組、ox-LDL對照組、不同劑量(0.01μmol/L,0.1μmol/L,1.0μmol/L,5.0 μmol/L)瑞舒伐他汀組,RT-PCR法測定各組肝X受體及小凹蛋白1mRNA的錶達.結果 空白對照組與ox-LDL對照組肝X受體mRNA的錶達分彆為1.00±0.02與0.26±0.02,兩組比較差異有統計學意義(t=56.392,P＜0.001);小凹蛋白1mRNA分彆為1.00±0.04與0.27±0.01,兩組比較差異有統計學意義(t=31.278,P＜0.001).ox-LDL對照組及不同劑量瑞舒伐他汀組間肝X受體及小凹蛋白1mRNA錶達比較,差異均有統計學意義(F值分彆為72.154、66.007,P均＜0.001),隨瑞舒伐他汀劑量的增加,肝X受體及小凹蛋白1mRNA錶達呈上升趨勢,兩兩比較差異均有統計學意義(P均＜0.05).結論 瑞舒伐他汀可上調單覈-巨噬細胞肝X受體mRNA、小凹蛋白1mRNA的錶達,且呈劑量依賴性.
목적 연구불동제량서서벌타정대양화수식저밀도지단백(ox-LDL)유도적인단핵거서세포간X수체급소요단백1표체적영향.방법 실험분6조:공백대조조、ox-LDL대조조、불동제량(0.01μmol/L,0.1μmol/L,1.0μmol/L,5.0 μmol/L)서서벌타정조,RT-PCR법측정각조간X수체급소요단백1mRNA적표체.결과 공백대조조여ox-LDL대조조간X수체mRNA적표체분별위1.00±0.02여0.26±0.02,량조비교차이유통계학의의(t=56.392,P＜0.001);소요단백1mRNA분별위1.00±0.04여0.27±0.01,량조비교차이유통계학의의(t=31.278,P＜0.001).ox-LDL대조조급불동제량서서벌타정조간간X수체급소요단백1mRNA표체비교,차이균유통계학의의(F치분별위72.154、66.007,P균＜0.001),수서서벌타정제량적증가,간X수체급소요단백1mRNA표체정상승추세,량량비교차이균유통계학의의(P균＜0.05).결론 서서벌타정가상조단핵-거서세포간X수체mRNA、소요단백1mRNA적표체,차정제량의뢰성.
Objective To investigate the effects of different doses of rosuvastatin on expression of liver X receptor(LXR) and caveolin-1 in cultured human monocyte-macrophage cells which induced by oxidized low density lipoprotein (ox-LDL).Methods The human monocyte-macrophage cells were divided into six groups:control group,ox-LDL group,different doses of rosuvastatin group (0.01 μmol/L,0.1 μmol/L,1 μmol/L,5 μmol/L).The expression of LXR mRNA and caveolin-1 mRNA were assayed by RT-PCR.Results LXR mRNA expression induced by ox-LDL in the control group and ox-LDL group were 1.00 ± 0.02,0.26 ± 0.02,and the difference was significant (t =56.39,P ＜ 0.001).Meanwhile,caveolin-1 mRNA expression in ox-LDL is (0.27 ± 0.01) fold than that in control (t =31.27,P ＜ 0.001).Meanwhile,There were significant differences among ox-LDL group and the different doses of rosuvastatin group in terms of LXR mRNA and caveolin-1 mRNA expressions (F =72.154,66.007,P ＜ 0.001).Along with the increase the doses of rosuvastatin,there was an increased trends of LXR mRNA and caveolin-1 mRNA expressions (P ＜ 0.05).Conclusion Rosuvastatin and upregulated the LXR mRNA and caveolin-1 mRNA expressions in a dose dependent manner.
