中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2014年
11期
1152-1155
,共4页
解学关%张梅%丁明胜%戴琰琨%孟胜东
解學關%張梅%丁明勝%戴琰琨%孟勝東
해학관%장매%정명성%대염곤%맹성동
血管内皮生长因子%碱性成纤维细胞生长因子%微球%高压氧%随意皮瓣
血管內皮生長因子%堿性成纖維細胞生長因子%微毬%高壓氧%隨意皮瓣
혈관내피생장인자%감성성섬유세포생장인자%미구%고압양%수의피판
Vascular endothelial growth factor%Basic fibroblast growth factor%Microspheres%Hyperbaric oxygen therapy%Uhralong random pattern flaps
目的 探讨血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)缓释微球联合高压氧对大鼠超长随意皮瓣成活率的影响.方法 采用改良的McFarlane皮瓣制作方法建立实验模型.模型制备后将SD大鼠40只随机分为4组(空白对照组、高压氧组、VEGF/bFGF微球组、高压氧与微球联合组),每组各10只,分别给予不同的干预处理.术后7d计算各组皮瓣的存活面积比,取皮瓣中段组织计算新生微血管数量,免疫组化法检测VEGF表达的差异.结果 (1)存活面积百分比:术后7d,高压氧与微球联合组皮瓣的存活面积百分比为(89.54±3.23)％,VEGF/bFGF微球组为(73.54±4.57)％,高压氧组为(71.89±2.26)％,空白对照组为(50.36±2.37)％,4组经方差分析,差异有统计学意义(F=390.328,P＜0.05),组间两两比较,高压氧与微球联合组显著高于其他实验组,差异有统计学意义(P均＜0.05);而VEGF/bFGF微球组和高压氧组间则无统计意义(P＞0.05).(2)新生微血管数目:高压氧与微球联合组、VEGF/bFGF微球组、高压氧组和空白对照组皮瓣Ⅱ区的新生血管计数分别为(35.14±4.21)、(23.34 ±2.53)、(25.22 ±2.73)、(17.37±5.73)个/mm2,4组经方差分析,差异有统计学意义(F=51.736,P＜0.05),组间两两比较,高压氧与微球联合组显著高于其他实验组,差异有统计学意义(P均＜0.05);VEGF/bFGF微球组与高压氧组间则无统计意义(P＞0.05).(3)免疫组化检测:各组VEGF阳性量累积吸光度(A)值分别为78.39±19.12、52.42±13.59、49.84±12.93、29.24±10.35,4组差异有统计学意义(F=189.956,P＜0.05),组间两两比较,高压氧与微球联合组显著高于其他实验组,差异有统计学意义(P均＜0.05);而VEGF/bFGF微球组与高压氧组间差异则无统计意义(P＞0.05).结论 VEGF和bFGF缓释微球联合高压氧可以促进皮瓣新生血管的增生,改善皮瓣血供,进而提高随意皮瓣的存活.
目的 探討血管內皮生長因子(VEGF)和堿性成纖維細胞生長因子(bFGF)緩釋微毬聯閤高壓氧對大鼠超長隨意皮瓣成活率的影響.方法 採用改良的McFarlane皮瓣製作方法建立實驗模型.模型製備後將SD大鼠40隻隨機分為4組(空白對照組、高壓氧組、VEGF/bFGF微毬組、高壓氧與微毬聯閤組),每組各10隻,分彆給予不同的榦預處理.術後7d計算各組皮瓣的存活麵積比,取皮瓣中段組織計算新生微血管數量,免疫組化法檢測VEGF錶達的差異.結果 (1)存活麵積百分比:術後7d,高壓氧與微毬聯閤組皮瓣的存活麵積百分比為(89.54±3.23)％,VEGF/bFGF微毬組為(73.54±4.57)％,高壓氧組為(71.89±2.26)％,空白對照組為(50.36±2.37)％,4組經方差分析,差異有統計學意義(F=390.328,P＜0.05),組間兩兩比較,高壓氧與微毬聯閤組顯著高于其他實驗組,差異有統計學意義(P均＜0.05);而VEGF/bFGF微毬組和高壓氧組間則無統計意義(P＞0.05).(2)新生微血管數目:高壓氧與微毬聯閤組、VEGF/bFGF微毬組、高壓氧組和空白對照組皮瓣Ⅱ區的新生血管計數分彆為(35.14±4.21)、(23.34 ±2.53)、(25.22 ±2.73)、(17.37±5.73)箇/mm2,4組經方差分析,差異有統計學意義(F=51.736,P＜0.05),組間兩兩比較,高壓氧與微毬聯閤組顯著高于其他實驗組,差異有統計學意義(P均＜0.05);VEGF/bFGF微毬組與高壓氧組間則無統計意義(P＞0.05).(3)免疫組化檢測:各組VEGF暘性量纍積吸光度(A)值分彆為78.39±19.12、52.42±13.59、49.84±12.93、29.24±10.35,4組差異有統計學意義(F=189.956,P＜0.05),組間兩兩比較,高壓氧與微毬聯閤組顯著高于其他實驗組,差異有統計學意義(P均＜0.05);而VEGF/bFGF微毬組與高壓氧組間差異則無統計意義(P＞0.05).結論 VEGF和bFGF緩釋微毬聯閤高壓氧可以促進皮瓣新生血管的增生,改善皮瓣血供,進而提高隨意皮瓣的存活.
목적 탐토혈관내피생장인자(VEGF)화감성성섬유세포생장인자(bFGF)완석미구연합고압양대대서초장수의피판성활솔적영향.방법 채용개량적McFarlane피판제작방법건립실험모형.모형제비후장SD대서40지수궤분위4조(공백대조조、고압양조、VEGF/bFGF미구조、고압양여미구연합조),매조각10지,분별급여불동적간예처리.술후7d계산각조피판적존활면적비,취피판중단조직계산신생미혈관수량,면역조화법검측VEGF표체적차이.결과 (1)존활면적백분비:술후7d,고압양여미구연합조피판적존활면적백분비위(89.54±3.23)％,VEGF/bFGF미구조위(73.54±4.57)％,고압양조위(71.89±2.26)％,공백대조조위(50.36±2.37)％,4조경방차분석,차이유통계학의의(F=390.328,P＜0.05),조간량량비교,고압양여미구연합조현저고우기타실험조,차이유통계학의의(P균＜0.05);이VEGF/bFGF미구조화고압양조간칙무통계의의(P＞0.05).(2)신생미혈관수목:고압양여미구연합조、VEGF/bFGF미구조、고압양조화공백대조조피판Ⅱ구적신생혈관계수분별위(35.14±4.21)、(23.34 ±2.53)、(25.22 ±2.73)、(17.37±5.73)개/mm2,4조경방차분석,차이유통계학의의(F=51.736,P＜0.05),조간량량비교,고압양여미구연합조현저고우기타실험조,차이유통계학의의(P균＜0.05);VEGF/bFGF미구조여고압양조간칙무통계의의(P＞0.05).(3)면역조화검측:각조VEGF양성량루적흡광도(A)치분별위78.39±19.12、52.42±13.59、49.84±12.93、29.24±10.35,4조차이유통계학의의(F=189.956,P＜0.05),조간량량비교,고압양여미구연합조현저고우기타실험조,차이유통계학의의(P균＜0.05);이VEGF/bFGF미구조여고압양조간차이칙무통계의의(P＞0.05).결론 VEGF화bFGF완석미구연합고압양가이촉진피판신생혈관적증생,개선피판혈공,진이제고수의피판적존활.
Objective To explore the effects of VEGF/bFGF sustained release microspheres combined with hyperbaric oxygen therapy on survival rates of uhralong random pattern flaps in rats.Methods Modified McFarlane flap rat models were randomly divided into 4 groups,VEGF/bFGF microspheres + hyperbaric oxygen group,VEGF/bFGF microspheres group,hyperbaric oxygen group and control group.At the end of 7 days,survival rates of flaps were calculated (Survival rate =Survival area/total area × 100％).The tissue samples were taken from middle portion of flaps to detect the neovascularization.Immunohistochemistry was used to detect the differential expression of VEGF.Results Seven days after the random pattern flap operation in rats,the survival rates of VEGF/bFGF microspheres combined with hyperbaric oxygen therapy experimental group,VEGF/bFGF microspheres experimental group,hyperbaric oxygen therapy experimental group,and control group were (89.54 ± 3.23) ％,(73.54 ± 4.57) ％,(71.89 ± 2.26) ％,(50.36 ± 2.37) ％ respectively.The neovascularization counts of each group in part Ⅱ were (35.14 ± 4.21)/mm2,(23.34 ± 2.53)/mm2,(25.22 ±2.73)/mm2 and (17.37 ± 5.73)/mm2 respectively.VEGF cumulative absorbance in VEGF/bFGF microspheres + hyperbaric oxygen group,VEGF/bFGF microspheres group,hyperbaric oxygen group and control group were 78.39 ± 9.12,52.42 ± 13.59,49.84 ± 12.93,29.24 ± 10.35 respectively.In each experimental result group,there were significant difference between the combination group and the other groups (F =189.956,P ＜ 0.05),but there was no significant difference between VEGF/bFGF microspheres experimental group and hyperbaric oxygen therapy experimental group.Conclusion VEGF/bFGF sustained release microspheres combined with hyperbaric oxygen therapy can promote flap neovascularization,increase the flap blood supply and then improve the survival of random pattern skin flaps.
